You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Ashwani Mittal,<br />
M.Sc. Ph.D (Protein Biochemistry)<br />
Assistant Professor (October 2003 to till date)<br />
ashmittal77@gmail.com<br />
uckkr.<strong>org</strong>/science.html<br />
Cell No: +919467466058<br />
Date of Birth: 2 nd October 1977<br />
Scientific Interests - Broad Research Areas:<br />
Muscle Metabolism, Protein Biochemistry and Cell Signaling<br />
Advance Research Training: Postdoc fellow (University of Loma Linda, California; University of<br />
Louisville, Kentucky, USA) - (March 2007 to August 2009)<br />
Professional Experiences:<br />
1 st Oct 2003 – till date- as an Assistant Professor in Biochemistry, University College,<br />
Kurukshetra University, Kurukshetra- India.<br />
Oct 2008 to Oct 2009- Second Postdoctoral training with a very well known scientist (<strong>Dr</strong> Ashok<br />
Kumar) in the area of skeletal muscular physiology.<br />
March 2007 to Oct 2008- First Postdoctoral training with well renowned scientist Prof Lawrence<br />
D Longo at Loma Linda University, CA in the field of vascular physiology and developmental<br />
study.<br />
30th March 2000 - 29th September 2003 as a Ph.D. Scholar, Department of Biochemistry,<br />
Kurukshetra University, Kurukshetra (KUK), India.<br />
13th August 1999 - 29th March 2000 as a Guest faculty, in University College, Kurukshetra<br />
University, Kurukshetra (KUK), India.<br />
Academic Credentials and achievements:<br />
Ph.D. (Biochemistry) from Department of Biochemistry, Kurukshetra University, Kurukshetra,<br />
India -2005. “Proteolytic Enzymes in Brain. Purification, Characterization and immobilization of<br />
Dipeptidyl peptidase IV (DPP IV) from Goat Brain”.<br />
M.Sc. (First Class) in Biochemistry from Kurukshetra University, Kurukshetra, with distinction in<br />
enzyme and metabolism, India - 1999.<br />
B.Sc. (Bachelor in Science) (First Class), Kurukshetra University, Kurukshetra, India - 1997.<br />
Qualified joint Council of Scientific and Industrial Research (CSIR)-University Grant<br />
Commission (UGC) test for Lectureship in June 1999--- A National level Competitive Test<br />
Qualified Graduate Aptitude Test for Engineering (GATE) – 2000 --A National level Competitive<br />
Test
Research projects (Collaborative work; 2007 – 2009)<br />
1. Role of cytokines in skeletal muscule Atrophy (NIH funded, USA)<br />
Tumor Necrosis Factor-related Weak Inducer of Apoptosis is a potent muscle-wasting cytokine<br />
which induces atrophy in cultured skeletal muscle cells. Worked as a Postdoc fellow<br />
2. Specific adrenergic receptors and PKC/ERKs isoforms in cerebral arterial contraction and their<br />
role in cerebral blood flow (NIH funded, USA) –<br />
Worked as a Postdoc fellow<br />
Other Research Contributions (2005-2007)<br />
<br />
<br />
<br />
Study of Dipeptidylaminopeptidases/ cathepsins/ xylanase enzymes by using different sources i.e.<br />
plants; microbial and animal brain for the purpose of its purification, enzyme kinetics and<br />
inhibition studies.<br />
Inhibition kinetics with some anti-tuberculous, anti-inflammatory and anti-leprotic drugs on<br />
cathepsins.<br />
“Immobilization of DPP IV and its application in Bio-transformation” (UGC funded Project,<br />
Delhi-India, 2006-2007).<br />
Current Research Project: (2012 – 2015)<br />
<br />
Effect of S-allyl cysteine on inflammatory and oxidative response in skeletal muscle metabolism<br />
(Funded by UGC, New Delhi, India – Rs 15 lacs)<br />
Representative publications<br />
Published Papers (http://www.refdoc.fr/NoticeresultatNoticeresultat2nbPageP=1 )<br />
THE REFERENCE IN SCIENTIFIC DOCUMENT SUPPLY (refdoc)<br />
(http://www.ncbi.nlm.nih.gov/pubmed)<br />
<br />
<br />
<br />
<br />
<br />
<br />
Bhatnagar S*, Mittal A* (first author), Gupta SK, Kumar A. TWEAK causes myotube atrophy<br />
through coordinated activation of ubiquitin-proteasome system, autophagy, and caspases. J Cell<br />
Physiol. 2012, 227(3).1042-51. PMID: 21567392.<br />
Aggarwal R, Sumran G, Kumar V, Mittal A. Copper(II) chloride mediated synthesis and DNA<br />
photocleavage activity of 1-aryl/heteroaryl-4-substituted-1,2,4-triazolo[4,3-a]quinoxalines. Eur J<br />
Med Chem. 2011, 46(12), 6083-8. PMID: 22051064<br />
Mittal A, Mandal TK, Bindal R and Dabur R. Cathepsin B is a negative regulator in pulmonary<br />
aspergillosis. IJBBB, 2011, 1(2): 125-130.<br />
Mittal A, Bhatnagar S, Kumar A, Paul PK, Kuang S and Kumar A. Genetic Ablation of TWEAK<br />
Augments Regeneration and Post-Injury Growth of Skeletal Muscle in Mice. American Journal of<br />
Pathology. 2010, Aug 19. [Epub ahead of print], PMID: 20724600<br />
Mittal A, Bhatnagar S, Kumar A, Lach-Trifilieff E, Wauters S, Li H, Makonchuk DY, Glass DJ,<br />
Kumar A. The TWEAK-Fn14 system is a critical regulator of denervation-induced skeletal muscle<br />
atrophy in mice. J Cell Biol. 2010 Mar 22;188(6):833-49. PMID: 20308426.<br />
Goyal R, Mittal A, Chu N, Arthur RA, Zhang L, Longo LD. Maturation and Long-Term Hypoxia-
Induced Acclimatization Responses in PKC-Mediated Signaling Pathways in Ovine Cerebral<br />
Arterial Contractility. Am J Physiol Regul Integr Comp Physiol. 2010 Aug 11. [Epub ahead of<br />
print] PMID: 20702800.<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
Goyal R, Mittal A, Chu N, Zhang L, Longo LD. Alpha(1)-Adrenergic receptor subtype function<br />
in fetal and adult cerebral arteries. Am J Physiol Heart Circ Physiol. 2010, 298(6):H1797-806.<br />
PMID: 20348219.<br />
Ghe<strong>org</strong>he CP, Goyal R, Mittal A, Longo LD. Gene expression in the placenta: maternal stress and<br />
epigenetic responses. Int J Dev Biol. 2010; 54(2-3):507-23. Review. PMID: 19876832.<br />
Sanghi A, Garg N, Gupta VK, Mittal, A, Kuhad RC. One-step Purification and Characterization<br />
of cellulase-free Xylanase Produced by Alkalophilic Bacillus Subtilis Ash. Brazilian Journal of<br />
Microbiology, 2010, 41(2), 467-476.<br />
Li H, Mittal A, Makonchuk DY, Bhatnagar S, Kumar A. Matrix Metalloproteinase-9 Inhibition<br />
Ameliorates Pathogenesis and Improves Skeletal Muscle Regeneration in Muscular Dystrophy.<br />
Hum Mol Genet. 2009 Apr 28. PMID: 19401296<br />
Li H, Mittal A, Paul PK, Kumar M, Srivastava DS, Tyagi SC, and Kumar A. Tumor necrosis<br />
factor-related weak inducer of apoptosis augments matrix metalloproteinase-9 production (MMP-<br />
9) in skeletal muscle through the activation of nuclear factor-kappa B-inducing kinase and p38<br />
mitogen-activated protein kinase: A potential role of MMP-9 in myopathy. J Biol Chem. 2009<br />
284(7):4439-50. PMID: 19074147<br />
Goyal R, Mittal A, Chu N, Shi L, Zhang L, Longo LD. Maturation and the role of PKC-mediated<br />
contractility in ovine cerebral arteries. Am J Physiol Heart Circ Physiol. 2009, 11. [Epub ahead of<br />
print] PMID: 19749163<br />
Goyal R, Galffy A, Field SA, Ghe<strong>org</strong>he CP, Mittal A, Longo LD. Maternal protein deprivation:<br />
changes in systemic renin-angiotensin system of the mouse fetus. Reprod Sci. 2009 16(9): 894-<br />
904. PMID: 19516079<br />
Kumar M, Makonchuk DY, Li H, Mittal A, and Kumar A. Tumor Necrosis Factor-like Weak<br />
Inducer of Apoptosis (TWEAK) activates proinflammatory signaling pathways and gene<br />
expression through the activation of TGF-beta activating kinase 1. J Immunol. 2009; 182(4):2439-<br />
48. PMID: 19201899.<br />
Sharma S, Mittal A, Gupta VK and Singh H. 2007. "Improved stabilization of microencapsulated<br />
Cathepsin B in harsh conditions" Enz Microb Tech, 40, 337-342.<br />
Sharma S, Mittal A, Gupta VK and Singh H. 2006. "Stabilization of immobilized Cathepsin L in<br />
Non-aqueous medium". Biocatalysis and Biotransformation, 18(2), 1-7.<br />
Sharma J, Singh A, Mittal A, Kumar A. 2006. Partial purification of an alkaline protease from a<br />
new strain of Aspergillus oryzae AWT 20 and its enhanced stabilization in entrapped Ca-alginate<br />
beads. Internet J Microbiology, 2(2).<br />
Mittal A, Singh H and Kamboj RC. 2005 "Characterization of Dipeptidyl Peptidase IV (DPP IV)<br />
in Ca-alginate beads. Enz Microb Tech, 37, 318-323.<br />
Sadana R, Mittal A, Khurana S, Singh H and Kamboj R C. 2003. Purification and characterization<br />
of cathepsin L-Iike from goat brain. Ind. J Biochem. Biophys. 40, 315-323<br />
Kamboj RC, Raghav N, Mittal A, Khurana S, Sadana R and Singh H. 2003. Effects of Some<br />
Antituberculous and AntiLeprotic <strong>Dr</strong>ugs on Cathepsins B, H and L. Ind. J. Clin. Biochem.18<br />
(2),39-47.
Mittal A, Khurana S, Sadana R, Kamboj RC and Singh H. 2003. Purification and characterization<br />
of DPP-IV from goat brain. J. Ind. Chem. Soc, 80, 1019-1025.<br />
Raghav N, Mittal A, Sadana R, Khurana S, Singh H and Kamboj RC. 2003. Effect of some antiinflarnmatof)',<br />
antituberculous and anti-Ieprotic drugs on Cathepsin H. lnd. J Hetero.Comp.<br />
13,41-44.<br />
Mittal A, Khurana S, Sadana R, Singh H and Kamboj RC. 2002. Regional and subcellular<br />
distribution of DPP-IV in goat brain. J. Ind. Chem. Soc. 79, 605-608.<br />
In News & some important citations<br />
<br />
TWEAKing Muscle Atrophy. Elizabeth M. Adler (30 March 2010). Sci. Signal. 3 (115), ec94.<br />
[DOI: 10.1126/scisignal.3115ec94].<br />
Disuse TWEAKs muscle loss. B. Short (2010). J. Cell Biol. 188, 752.<br />
<br />
<br />
Science Daily, March 22, 2010. TWEAK Triggers Atrophy of Disused Muscle.<br />
http://www.sciencedaily.com/releases/2010/03/100322091956.htm<br />
Eurekalert, March 22, 2010. TWEAK Triggers Atrophy of Disused Muscle.<br />
http://www.eurekalert.<strong>org</strong>/pub_releases/2010-03/rup-tta031210.php<br />
Abstracts/Papers (National/International Conferences):<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
Mittal A. Effect of natural compounds on inflammatory and oxidative response in skeletal muscle<br />
physiology. (In the proceedings of National Symposium on Emerging Trends in Biotechnology,<br />
M.M. Modi College, Feb 24 th , 2012).<br />
Mittal A, Bindal R, Dabur R. 2011. Cathepsin B: A possible negative regulator in fungal mediated<br />
lung infection. (In the proceedings of International Conference on Food Engineering and<br />
Biotechnology-ICFEB, Bangkok, Thailand. May 28 th – 29 th , 2011).<br />
Kumar A, Bhatnagar S, Mittal A and Glass DJ. 2010. TWEAK/Fn14 system is a critical regulator<br />
of denervation-induced skeletal muscle atrophy.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/24/1_MeetingAbstracts/801.30<br />
Mittal A and Kumar A. 2009. TWEAK-induces muscle-wasting through coordinative action of<br />
autophagy, caspases and ubiquitin-proteosome proteolysis systems. (In proceedings of “Making<br />
Muscle in the Embryo and the Adult, Columbia University, New York, NY, USA – May 28 to<br />
June 2, 2009).<br />
Kumar A, Li H, Makonchuk DY, Bhatnagar S, Ashkenazi A, Mittal A. 2009. TWEAK/Fn14<br />
system is an important regulator of loss of skeletal muscle mass in response to nerve injury. (In<br />
proceedings of “Making Muscle in the Embryo and the Adult, Columbia University, New York,<br />
NY, USA – May 28 to June 2, 2009).<br />
Mittal A and Kumar A. 2009. TWEAK-induces muscle-wasting through coordinative action of<br />
autophagy and ubiquitin-proteosome proteolysis systems. (In proceedings of “Understanding<br />
Muscle: From Development to Disease, Ohio State University, Columbus, Ohio, April,16).<br />
Mittal A, Li H, Makonchuk DY, Bhatnagar S, Ashkenazi A, Kumar A. 2009. TWEAK is an<br />
important mediator of progressive fibrosis. Fiber-type switching and loss of skeletal muscle mass<br />
in response to nerve injury. (In proceedings of “Understanding Muscle: From Development to<br />
Disease, Ohio State University, Columbus, Ohio, April,16).
Li H, Mittal A, Makonchuk DY, Bhatnagar S, Kumar A. 2009. Matrix Metalloproteinase-9<br />
Inhibition Ameliorates Pathogenesis and Improves Skeletal Muscle Regeneration in Muscular<br />
Dystrophy. (In proceedings of “Understanding Muscle: From Development to Disease, Ohio State<br />
University, Columbus, Ohio, April,16).<br />
Mittal A, Goyal R, Wedhas N, Shi L and Longo LD. 2009. Differential role of {alpha}1-<br />
adrenergic receptor subtypes in Ca2+-dependent and Ca2+-independent contraction in developing<br />
cerebral arteries. (New Orleans, USA). The FASEB Journal. 2009;23:613.23<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/613.23<br />
Mittal A, Goyal R, Shi L, Wedhas N and Longo LD. 2009. 2009. Role of ERK1/2 and Rho A/Rho<br />
kinase in PKC-induced contractions in ovine cerebral arteries: A developmental study. (New<br />
Orleans, USA). The FASEB Journal. 2009;23:613.26.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/613.26<br />
Goyal R, Mittal A, Nguyhn D, Loftin M, Derrick A, Pearce WJ, Longo LD and Wilson SM. 2009.<br />
Roles of PKC, RhoA and ERK signaling to serotonergic contractility of pulmonary arteries from<br />
chronic hypoxic fetal and adult sheep. (New Orleans, USA). The FASEB Journal. 2009;23:619.5.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.5<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
Goyal R, Mittal A, Shi L, Wedhas N and Longo LD. 2009. High altitude acclimatization<br />
responses in cerebral artery PKC-dependent signaling pathway proteins: Changes with<br />
development. (New Orleans, USA). The FASEB Journal. 2009;23:616.14.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/616.14<br />
Goyal R, Vemulakonda S, Mittal A, Loftin M, Vranken K, Nguyen D, Webster M, Blood AB,<br />
Pearce WJ, Longo LD and Wilson SM (2009). Maternal chronic hypoxic stress influences the role<br />
of extracellular Ca2+ influx to serotonin-mediated contractility of pulmonary arteries from fetal<br />
sheep (New Orleans, USA). The FASEB Journal. 2009;23:619.4.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.4<br />
Goyal R, Vemulakonda S, Mittal A, Loftin M, Vranken K, Nguyen D, Webster M, Blood AB,<br />
Pearce WJ, Longo LD and Wilson SM (2009). Chronic hypoxia influences the role of extracellular<br />
Ca2+ influx during serotonin-mediated contractility of pulmonary arteries from adult sheep. (New<br />
Orleans, USA). The FASEB Journal. 2009;23:619.6.<br />
http://www.fasebj.<strong>org</strong>/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.6<br />
Field S, Galffy A, Wright TA, Jang A, Goyal R, Mittal A, Longo L (2008). Maternal protein<br />
deprivation decreases levels of fetal liver angiotensinogen. (Proceeding of 2008 Health Disparities<br />
Research Symposium, Loma Linda University, CA, USA, August 6, pp 42).<br />
Galffy A, Mittal A, Goyal R, Wright TA, Jang EE, Longo LD (2008). Maternal low protein diet:<br />
Effect on fetal cardiac AT1 and AT2 receptors. (Proceeding of 2008 Health Disparities Research<br />
Symposium, Loma Linda University, CA, USA, August 6, pp 48).<br />
Jang EE, Goyal R, Mittal A, Wright TA, Galffy A, Longo LD (2008). Maternal protein<br />
deprivation and the epigenetic origin of hypertension. Fetal mouse kideny’s Renin-Angiotensin<br />
system. (Proceeding of 2008 Health Disparities Research Symposium, Loma Linda University,<br />
CA, USA, August 6, pp 56).<br />
Wright TA, Galffy A, Field S, Jang EE, Goyal R, Mittal A, Longo LD (2008). Maternal low<br />
protein diet: Effect on fetal ACE I & ACE II and the fetal origin of hypertension. (Proceeding of<br />
2008 Health Disparities Research Symposium, Loma Linda University, CA, USA, August 6, pp<br />
124).<br />
Sharma S, Gupta V K, Singh H, Mittal A (2006). Stabilization of Cathepsin B in non-aqueous<br />
medium Proceeding in International conference of Molecules to Materials (ICMM-06), Panjab,
Longowal, India.<br />
Sharma S, Mittal A, Gupta VK, Singh H (2007). Covalently immobilization of cathepsin via<br />
gluteraldehyde to purified chitosan: a simple procedure (proceedings in 94 th Indian Science<br />
Congress, Anna Malai University, Tamilnadu, India.<br />
Sharma S, Mittal A, Gupta VK, Singh H (2005) Microencapsulation of Cathepsin B in CM-<br />
Cellulose-Ca-Alginate capsules, (Proceedings in 93 rd Indian Science Congress, Hydrabad).<br />
Sarina, Sharma S, Mittal A, Gupta VK, Singh H (2005), Physico-chemical properties of purified<br />
mung bean cathepsin B-like enzyme. (Proceedings in 93 rd Indian Science Congress, Hydrabad).<br />
Rita, Mittal A, Gupta VK and Singh H. (2005) Immobilization of cathepsin-L in Calcium<br />
Alginate Beads. Proceedings in 92nd Indian Science Congress (Ahemdabad).<br />
Mittal A, Kamboj RC and Singh H. (2004) Physico-chemical Properties of purified Goat Brain<br />
DPP IV enzyme. Proceedings 91th Indian Science Congress (Chandigarh)<br />
Kamboj RC, Mittal A and Singh H. (2004) Immobilization of Dipeptidyl Peptidase IV (DPP IV)<br />
In Calcium Alginate Beads (Panjab, Longowal).<br />
Mittal A, Khurana S, Singh H and Kamboj RC. (2003) Effects of Some Antituberculous and<br />
Antileprotic <strong>Dr</strong>ugs on cysteinc proteinases. 90th Indian Science Congress (Bangalore).<br />
Mittal A, Singh H and Kamboj RC (2003) Inhibition of Dipeptidylpeptidase IV (DPP IV) by<br />
Phenylmethane sulphonylfluoride. 40th Annual Convention of Indian Chemical Society (Janshi).<br />
Mittal A, Khurana S, Sadana R, Singh H and Kamboj RC. (2002) Purification and<br />
characterization of DPP-IV from goat brain. 89th Indian Science Congress. Part-III, pp 5.<br />
Khurana S, Mittal A, Singh H and Kamboj RC. (2002) Physico-chemical properties of<br />
dipeptidylpeptidase II from goat brain. 71 SI Annual Society of Biological Chemist (SBC).<br />
Rajesh, Singh H, Mittal A, Sharma GL. (2001) Effects of a novel antifungal molecule on<br />
interleukin. 9th Asian- Pacific Congress of Clinical Biochemistry-New Delhi.<br />
Singh H, Khurana S, Mittal A, Sadana R and Kamboj RC. (2001) Purification and<br />
characterization of DPP-II from goat brain. 38th Annual Convention of Indian Chemical Society.<br />
OP-13.<br />
Conferences/workshops/Seminars (International/National)<br />
‣ National Symposium on Emerging Trends in Biotechnology, M.M. Modi College, Feb 24 th , 2012<br />
‣ Making Muscle in the Embryo and the Adult, Columbia University, New York, NY, USA –<br />
May 28 to June 2, 2009.<br />
‣ Understanding Muscle: From Development to Disease, Ohio State University, Columbus,<br />
Ohio, April,16 2009.<br />
‣ Health Disparities Research Symposium, Loma Linda University, CA, USA, August 6, 2008.<br />
‣ Workshop/Orientation course on “Laboratory methods in gene transfer and gene expression”<br />
(MICR, 536), at Loma Linda University, Loma Linda, CA, USA (18 th June – 29 th June 2007).<br />
‣ “Proteomics: Principles and Methods”- Trac 25, A Biotechnology (BioTrac) workshop/training<br />
course conducted by foundation for advanced education in the sciences (FAES) at National<br />
Institute of Health (NIH), in Bethesda, MD, USA. (1 st oct to 5 th Oct 2007).<br />
‣ International Conference on Molecules to Materials-06, SLIET, Longowal, Panjab.
‣ 88 th Indian Science Congress Association (IARI- New Delhi) – 2001.<br />
‣ 89 th Indian Science Congress Association (Lucknow University) – 2002.<br />
‣ Workshop on "Recent Trends in Genomics and Proteomics" <strong>org</strong>anized by Bioinformatics<br />
centre, H.P. Universitv. Shimla – 2002.<br />
‣ 90 th Indian Science Congress Association (Bangalore University) – 2003.<br />
‣ 91 st Indian Science Congress Association (Panjab University) – 2004<br />
‣ 92 nd Indian Science Congress Association (Nirma University, Ahemdabad) - 2005<br />
‣ 93 rd Indian Science Congress Association (Acharya Ranga University, Hyderabad-2006)<br />
‣ 94 th Indian Sceince Congress Association (Anna Malai University, Tamil Nadu -2007, India)<br />
‣ "Patent Awareness Workshop" <strong>org</strong>anized by Punjab State Council for Science and Technology<br />
and Biochemistry Dept., K.U.K.<br />
‣ Seminar-cum-training on "Electron Microscope" <strong>org</strong>anized by SAIF (P.U. Chandigarh) and<br />
Zoology Dept., K.U.K.<br />
‣ Seminar on "Emerging Trends in Biotechnology" <strong>org</strong>anized by Sartorius group (German) and<br />
Biotechnology Dept., K.UK<br />
‣ Workshop on "Trends in Computational Biology" <strong>org</strong>anized by Bioinformatics centre, NDRI,<br />
Karnal, INDIA, 18 th Oct – 20 th Oct 2005.<br />
‣ Workshop on "Advances in Bio-informatics" <strong>org</strong>anized by Bioinformatics Centre, at GNDU,<br />
Amritsar, Panjab from 23 rd Feb – 24 th Feb 2006.<br />
Invited lectures:<br />
<br />
“A Molecular Tool for the Authentication of Medicinal Plants: DNA Fingerprinting”, on the<br />
occasion of Reorientation Training Program (Sanctioned by Dept. of AYUSH, M/o Health & F W,<br />
Govt. of India) at Regional Research Institute (Ay) Kothrud, Pune. (12.12.2009).<br />
<br />
<br />
<br />
“DNA fingerprinting: A molecular tool for the authentication of herbal plants”, on the occasion of<br />
Reorientation Training Program (Sanctioned by Dept. of AYUSH, M/o Health & F W, Govt. of<br />
India) at Regional Research Institute (Ay) Kothrud, Pune. (27.02.2010).<br />
Lecture delivered on topic entitled, “Overview of Carbohydrate Metabolism” to the PG students<br />
of Biochemistry/Biotechnology/Biosciences departments at Dolphin (PG) Institute of Bio-Medical<br />
& Natural Sciences, Dehradun, Uttarakhand. (12.08.2010)<br />
Lecture delivered on topic entitled, “Purification of protein and Criteria of their Purity” to the PG<br />
students of Biochemistry/Biotechnology/Biosciences departments at Dolphin (PG) Institute of<br />
Bio-Medical & Natural Sciences, Dehradun, Uttarakhand. (29.03.2011)<br />
Hands on Experience with the Techniques:<br />
Electrophoresis, Column Chromatography (Gel filtration, Affinity and ion exchange), ELISA,<br />
Isoelectrofocusing, Western Blotting, Co-immunoprecipitation, GST-, His-pull down assay,<br />
Double immunoflourescence, Histochemistry, Electrophoretic mobility shift assay (EMSA),<br />
muscle cell culture, denervation, cardiotoxin, and skeletal muscle cell culture etc.<br />
Teaching expertise:<br />
<br />
Protein Biochemistry, Enzyme kinetics and Metabolism, Molecular Biology & Immunology