Dr Ashwini Mital - Uckkr.org

Ashwani Mittal,
M.Sc. Ph.D (Protein Biochemistry)
Assistant Professor (October 2003 to till date)
ashmittal77@gmail.com
uckkr.org/science.html
Cell No: +919467466058
Date of Birth: 2 nd October 1977
Scientific Interests - Broad Research Areas:
Muscle Metabolism, Protein Biochemistry and Cell Signaling
Advance Research Training: Postdoc fellow (University of Loma Linda, California; University of
Louisville, Kentucky, USA) - (March 2007 to August 2009)
Professional Experiences:
1 st Oct 2003 – till date- as an Assistant Professor in Biochemistry, University College,
Kurukshetra University, Kurukshetra- India.
Oct 2008 to Oct 2009- Second Postdoctoral training with a very well known scientist (Dr Ashok
Kumar) in the area of skeletal muscular physiology.
March 2007 to Oct 2008- First Postdoctoral training with well renowned scientist Prof Lawrence
D Longo at Loma Linda University, CA in the field of vascular physiology and developmental
study.
30th March 2000 - 29th September 2003 as a Ph.D. Scholar, Department of Biochemistry,
Kurukshetra University, Kurukshetra (KUK), India.
13th August 1999 - 29th March 2000 as a Guest faculty, in University College, Kurukshetra
University, Kurukshetra (KUK), India.
Academic Credentials and achievements:
Ph.D. (Biochemistry) from Department of Biochemistry, Kurukshetra University, Kurukshetra,
India -2005. “Proteolytic Enzymes in Brain. Purification, Characterization and immobilization of
Dipeptidyl peptidase IV (DPP IV) from Goat Brain”.
M.Sc. (First Class) in Biochemistry from Kurukshetra University, Kurukshetra, with distinction in
enzyme and metabolism, India - 1999.
B.Sc. (Bachelor in Science) (First Class), Kurukshetra University, Kurukshetra, India - 1997.
Qualified joint Council of Scientific and Industrial Research (CSIR)-University Grant
Commission (UGC) test for Lectureship in June 1999--- A National level Competitive Test
Qualified Graduate Aptitude Test for Engineering (GATE) – 2000 --A National level Competitive
Test

Research projects (Collaborative work; 2007 – 2009)
1. Role of cytokines in skeletal muscule Atrophy (NIH funded, USA)
Tumor Necrosis Factor-related Weak Inducer of Apoptosis is a potent muscle-wasting cytokine
which induces atrophy in cultured skeletal muscle cells. Worked as a Postdoc fellow
2. Specific adrenergic receptors and PKC/ERKs isoforms in cerebral arterial contraction and their
role in cerebral blood flow (NIH funded, USA) –
Worked as a Postdoc fellow
Other Research Contributions (2005-2007)
Study of Dipeptidylaminopeptidases/ cathepsins/ xylanase enzymes by using different sources i.e.
plants; microbial and animal brain for the purpose of its purification, enzyme kinetics and
inhibition studies.
Inhibition kinetics with some anti-tuberculous, anti-inflammatory and anti-leprotic drugs on
cathepsins.
“Immobilization of DPP IV and its application in Bio-transformation” (UGC funded Project,
Delhi-India, 2006-2007).
Current Research Project: (2012 – 2015)
Effect of S-allyl cysteine on inflammatory and oxidative response in skeletal muscle metabolism
(Funded by UGC, New Delhi, India – Rs 15 lacs)
Representative publications
Published Papers (http://www.refdoc.fr/NoticeresultatNoticeresultat2nbPageP=1 )
THE REFERENCE IN SCIENTIFIC DOCUMENT SUPPLY (refdoc)
(http://www.ncbi.nlm.nih.gov/pubmed)
Bhatnagar S*, Mittal A* (first author), Gupta SK, Kumar A. TWEAK causes myotube atrophy
through coordinated activation of ubiquitin-proteasome system, autophagy, and caspases. J Cell
Physiol. 2012, 227(3).1042-51. PMID: 21567392.
Aggarwal R, Sumran G, Kumar V, Mittal A. Copper(II) chloride mediated synthesis and DNA
photocleavage activity of 1-aryl/heteroaryl-4-substituted-1,2,4-triazolo[4,3-a]quinoxalines. Eur J
Med Chem. 2011, 46(12), 6083-8. PMID: 22051064
Mittal A, Mandal TK, Bindal R and Dabur R. Cathepsin B is a negative regulator in pulmonary
aspergillosis. IJBBB, 2011, 1(2): 125-130.
Mittal A, Bhatnagar S, Kumar A, Paul PK, Kuang S and Kumar A. Genetic Ablation of TWEAK
Augments Regeneration and Post-Injury Growth of Skeletal Muscle in Mice. American Journal of
Pathology. 2010, Aug 19. [Epub ahead of print], PMID: 20724600
Mittal A, Bhatnagar S, Kumar A, Lach-Trifilieff E, Wauters S, Li H, Makonchuk DY, Glass DJ,
Kumar A. The TWEAK-Fn14 system is a critical regulator of denervation-induced skeletal muscle
atrophy in mice. J Cell Biol. 2010 Mar 22;188(6):833-49. PMID: 20308426.
Goyal R, Mittal A, Chu N, Arthur RA, Zhang L, Longo LD. Maturation and Long-Term Hypoxia-

Induced Acclimatization Responses in PKC-Mediated Signaling Pathways in Ovine Cerebral
Arterial Contractility. Am J Physiol Regul Integr Comp Physiol. 2010 Aug 11. [Epub ahead of
print] PMID: 20702800.
Goyal R, Mittal A, Chu N, Zhang L, Longo LD. Alpha(1)-Adrenergic receptor subtype function
in fetal and adult cerebral arteries. Am J Physiol Heart Circ Physiol. 2010, 298(6):H1797-806.
PMID: 20348219.
Gheorghe CP, Goyal R, Mittal A, Longo LD. Gene expression in the placenta: maternal stress and
epigenetic responses. Int J Dev Biol. 2010; 54(2-3):507-23. Review. PMID: 19876832.
Sanghi A, Garg N, Gupta VK, Mittal, A, Kuhad RC. One-step Purification and Characterization
of cellulase-free Xylanase Produced by Alkalophilic Bacillus Subtilis Ash. Brazilian Journal of
Microbiology, 2010, 41(2), 467-476.
Li H, Mittal A, Makonchuk DY, Bhatnagar S, Kumar A. Matrix Metalloproteinase-9 Inhibition
Ameliorates Pathogenesis and Improves Skeletal Muscle Regeneration in Muscular Dystrophy.
Hum Mol Genet. 2009 Apr 28. PMID: 19401296
Li H, Mittal A, Paul PK, Kumar M, Srivastava DS, Tyagi SC, and Kumar A. Tumor necrosis
factor-related weak inducer of apoptosis augments matrix metalloproteinase-9 production (MMP-
9) in skeletal muscle through the activation of nuclear factor-kappa B-inducing kinase and p38
mitogen-activated protein kinase: A potential role of MMP-9 in myopathy. J Biol Chem. 2009
284(7):4439-50. PMID: 19074147
Goyal R, Mittal A, Chu N, Shi L, Zhang L, Longo LD. Maturation and the role of PKC-mediated
contractility in ovine cerebral arteries. Am J Physiol Heart Circ Physiol. 2009, 11. [Epub ahead of
print] PMID: 19749163
Goyal R, Galffy A, Field SA, Gheorghe CP, Mittal A, Longo LD. Maternal protein deprivation:
changes in systemic renin-angiotensin system of the mouse fetus. Reprod Sci. 2009 16(9): 894-
904. PMID: 19516079
Kumar M, Makonchuk DY, Li H, Mittal A, and Kumar A. Tumor Necrosis Factor-like Weak
Inducer of Apoptosis (TWEAK) activates proinflammatory signaling pathways and gene
expression through the activation of TGF-beta activating kinase 1. J Immunol. 2009; 182(4):2439-
48. PMID: 19201899.
Sharma S, Mittal A, Gupta VK and Singh H. 2007. "Improved stabilization of microencapsulated
Cathepsin B in harsh conditions" Enz Microb Tech, 40, 337-342.
Sharma S, Mittal A, Gupta VK and Singh H. 2006. "Stabilization of immobilized Cathepsin L in
Non-aqueous medium". Biocatalysis and Biotransformation, 18(2), 1-7.
Sharma J, Singh A, Mittal A, Kumar A. 2006. Partial purification of an alkaline protease from a
new strain of Aspergillus oryzae AWT 20 and its enhanced stabilization in entrapped Ca-alginate
beads. Internet J Microbiology, 2(2).
Mittal A, Singh H and Kamboj RC. 2005 "Characterization of Dipeptidyl Peptidase IV (DPP IV)
in Ca-alginate beads. Enz Microb Tech, 37, 318-323.
Sadana R, Mittal A, Khurana S, Singh H and Kamboj R C. 2003. Purification and characterization
of cathepsin L-Iike from goat brain. Ind. J Biochem. Biophys. 40, 315-323
Kamboj RC, Raghav N, Mittal A, Khurana S, Sadana R and Singh H. 2003. Effects of Some
Antituberculous and AntiLeprotic Drugs on Cathepsins B, H and L. Ind. J. Clin. Biochem.18
(2),39-47.

Mittal A, Khurana S, Sadana R, Kamboj RC and Singh H. 2003. Purification and characterization
of DPP-IV from goat brain. J. Ind. Chem. Soc, 80, 1019-1025.
Raghav N, Mittal A, Sadana R, Khurana S, Singh H and Kamboj RC. 2003. Effect of some antiinflarnmatof)',
antituberculous and anti-Ieprotic drugs on Cathepsin H. lnd. J Hetero.Comp.
13,41-44.
Mittal A, Khurana S, Sadana R, Singh H and Kamboj RC. 2002. Regional and subcellular
distribution of DPP-IV in goat brain. J. Ind. Chem. Soc. 79, 605-608.
In News & some important citations
TWEAKing Muscle Atrophy. Elizabeth M. Adler (30 March 2010). Sci. Signal. 3 (115), ec94.
[DOI: 10.1126/scisignal.3115ec94].
Disuse TWEAKs muscle loss. B. Short (2010). J. Cell Biol. 188, 752.
Science Daily, March 22, 2010. TWEAK Triggers Atrophy of Disused Muscle.
http://www.sciencedaily.com/releases/2010/03/100322091956.htm
Eurekalert, March 22, 2010. TWEAK Triggers Atrophy of Disused Muscle.
http://www.eurekalert.org/pub_releases/2010-03/rup-tta031210.php
Abstracts/Papers (National/International Conferences):
Mittal A. Effect of natural compounds on inflammatory and oxidative response in skeletal muscle
physiology. (In the proceedings of National Symposium on Emerging Trends in Biotechnology,
M.M. Modi College, Feb 24 th , 2012).
Mittal A, Bindal R, Dabur R. 2011. Cathepsin B: A possible negative regulator in fungal mediated
lung infection. (In the proceedings of International Conference on Food Engineering and
Biotechnology-ICFEB, Bangkok, Thailand. May 28 th – 29 th , 2011).
Kumar A, Bhatnagar S, Mittal A and Glass DJ. 2010. TWEAK/Fn14 system is a critical regulator
of denervation-induced skeletal muscle atrophy.
http://www.fasebj.org/cgi/content/meeting_abstract/24/1_MeetingAbstracts/801.30
Mittal A and Kumar A. 2009. TWEAK-induces muscle-wasting through coordinative action of
autophagy, caspases and ubiquitin-proteosome proteolysis systems. (In proceedings of “Making
Muscle in the Embryo and the Adult, Columbia University, New York, NY, USA – May 28 to
June 2, 2009).
Kumar A, Li H, Makonchuk DY, Bhatnagar S, Ashkenazi A, Mittal A. 2009. TWEAK/Fn14
system is an important regulator of loss of skeletal muscle mass in response to nerve injury. (In
proceedings of “Making Muscle in the Embryo and the Adult, Columbia University, New York,
NY, USA – May 28 to June 2, 2009).
Mittal A and Kumar A. 2009. TWEAK-induces muscle-wasting through coordinative action of
autophagy and ubiquitin-proteosome proteolysis systems. (In proceedings of “Understanding
Muscle: From Development to Disease, Ohio State University, Columbus, Ohio, April,16).
Mittal A, Li H, Makonchuk DY, Bhatnagar S, Ashkenazi A, Kumar A. 2009. TWEAK is an
important mediator of progressive fibrosis. Fiber-type switching and loss of skeletal muscle mass
in response to nerve injury. (In proceedings of “Understanding Muscle: From Development to
Disease, Ohio State University, Columbus, Ohio, April,16).

Li H, Mittal A, Makonchuk DY, Bhatnagar S, Kumar A. 2009. Matrix Metalloproteinase-9
Inhibition Ameliorates Pathogenesis and Improves Skeletal Muscle Regeneration in Muscular
Dystrophy. (In proceedings of “Understanding Muscle: From Development to Disease, Ohio State
University, Columbus, Ohio, April,16).
Mittal A, Goyal R, Wedhas N, Shi L and Longo LD. 2009. Differential role of {alpha}1-
adrenergic receptor subtypes in Ca2+-dependent and Ca2+-independent contraction in developing
cerebral arteries. (New Orleans, USA). The FASEB Journal. 2009;23:613.23
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/613.23
Mittal A, Goyal R, Shi L, Wedhas N and Longo LD. 2009. 2009. Role of ERK1/2 and Rho A/Rho
kinase in PKC-induced contractions in ovine cerebral arteries: A developmental study. (New
Orleans, USA). The FASEB Journal. 2009;23:613.26.
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/613.26
Goyal R, Mittal A, Nguyhn D, Loftin M, Derrick A, Pearce WJ, Longo LD and Wilson SM. 2009.
Roles of PKC, RhoA and ERK signaling to serotonergic contractility of pulmonary arteries from
chronic hypoxic fetal and adult sheep. (New Orleans, USA). The FASEB Journal. 2009;23:619.5.
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.5
Goyal R, Mittal A, Shi L, Wedhas N and Longo LD. 2009. High altitude acclimatization
responses in cerebral artery PKC-dependent signaling pathway proteins: Changes with
development. (New Orleans, USA). The FASEB Journal. 2009;23:616.14.
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/616.14
Goyal R, Vemulakonda S, Mittal A, Loftin M, Vranken K, Nguyen D, Webster M, Blood AB,
Pearce WJ, Longo LD and Wilson SM (2009). Maternal chronic hypoxic stress influences the role
of extracellular Ca2+ influx to serotonin-mediated contractility of pulmonary arteries from fetal
sheep (New Orleans, USA). The FASEB Journal. 2009;23:619.4.
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.4
Goyal R, Vemulakonda S, Mittal A, Loftin M, Vranken K, Nguyen D, Webster M, Blood AB,
Pearce WJ, Longo LD and Wilson SM (2009). Chronic hypoxia influences the role of extracellular
Ca2+ influx during serotonin-mediated contractility of pulmonary arteries from adult sheep. (New
Orleans, USA). The FASEB Journal. 2009;23:619.6.
http://www.fasebj.org/cgi/content/meeting_abstract/23/1_MeetingAbstracts/619.6
Field S, Galffy A, Wright TA, Jang A, Goyal R, Mittal A, Longo L (2008). Maternal protein
deprivation decreases levels of fetal liver angiotensinogen. (Proceeding of 2008 Health Disparities
Research Symposium, Loma Linda University, CA, USA, August 6, pp 42).
Galffy A, Mittal A, Goyal R, Wright TA, Jang EE, Longo LD (2008). Maternal low protein diet:
Effect on fetal cardiac AT1 and AT2 receptors. (Proceeding of 2008 Health Disparities Research
Symposium, Loma Linda University, CA, USA, August 6, pp 48).
Jang EE, Goyal R, Mittal A, Wright TA, Galffy A, Longo LD (2008). Maternal protein
deprivation and the epigenetic origin of hypertension. Fetal mouse kideny’s Renin-Angiotensin
system. (Proceeding of 2008 Health Disparities Research Symposium, Loma Linda University,
CA, USA, August 6, pp 56).
Wright TA, Galffy A, Field S, Jang EE, Goyal R, Mittal A, Longo LD (2008). Maternal low
protein diet: Effect on fetal ACE I & ACE II and the fetal origin of hypertension. (Proceeding of
2008 Health Disparities Research Symposium, Loma Linda University, CA, USA, August 6, pp
124).
Sharma S, Gupta V K, Singh H, Mittal A (2006). Stabilization of Cathepsin B in non-aqueous
medium Proceeding in International conference of Molecules to Materials (ICMM-06), Panjab,

Longowal, India.
Sharma S, Mittal A, Gupta VK, Singh H (2007). Covalently immobilization of cathepsin via
gluteraldehyde to purified chitosan: a simple procedure (proceedings in 94 th Indian Science
Congress, Anna Malai University, Tamilnadu, India.
Sharma S, Mittal A, Gupta VK, Singh H (2005) Microencapsulation of Cathepsin B in CM-
Cellulose-Ca-Alginate capsules, (Proceedings in 93 rd Indian Science Congress, Hydrabad).
Sarina, Sharma S, Mittal A, Gupta VK, Singh H (2005), Physico-chemical properties of purified
mung bean cathepsin B-like enzyme. (Proceedings in 93 rd Indian Science Congress, Hydrabad).
Rita, Mittal A, Gupta VK and Singh H. (2005) Immobilization of cathepsin-L in Calcium
Alginate Beads. Proceedings in 92nd Indian Science Congress (Ahemdabad).
Mittal A, Kamboj RC and Singh H. (2004) Physico-chemical Properties of purified Goat Brain
DPP IV enzyme. Proceedings 91th Indian Science Congress (Chandigarh)
Kamboj RC, Mittal A and Singh H. (2004) Immobilization of Dipeptidyl Peptidase IV (DPP IV)
In Calcium Alginate Beads (Panjab, Longowal).
Mittal A, Khurana S, Singh H and Kamboj RC. (2003) Effects of Some Antituberculous and
Antileprotic Drugs on cysteinc proteinases. 90th Indian Science Congress (Bangalore).
Mittal A, Singh H and Kamboj RC (2003) Inhibition of Dipeptidylpeptidase IV (DPP IV) by
Phenylmethane sulphonylfluoride. 40th Annual Convention of Indian Chemical Society (Janshi).
Mittal A, Khurana S, Sadana R, Singh H and Kamboj RC. (2002) Purification and
characterization of DPP-IV from goat brain. 89th Indian Science Congress. Part-III, pp 5.
Khurana S, Mittal A, Singh H and Kamboj RC. (2002) Physico-chemical properties of
dipeptidylpeptidase II from goat brain. 71 SI Annual Society of Biological Chemist (SBC).
Rajesh, Singh H, Mittal A, Sharma GL. (2001) Effects of a novel antifungal molecule on
interleukin. 9th Asian- Pacific Congress of Clinical Biochemistry-New Delhi.
Singh H, Khurana S, Mittal A, Sadana R and Kamboj RC. (2001) Purification and
characterization of DPP-II from goat brain. 38th Annual Convention of Indian Chemical Society.
OP-13.
Conferences/workshops/Seminars (International/National)
‣ National Symposium on Emerging Trends in Biotechnology, M.M. Modi College, Feb 24 th , 2012
‣ Making Muscle in the Embryo and the Adult, Columbia University, New York, NY, USA –
May 28 to June 2, 2009.
‣ Understanding Muscle: From Development to Disease, Ohio State University, Columbus,
Ohio, April,16 2009.
‣ Health Disparities Research Symposium, Loma Linda University, CA, USA, August 6, 2008.
‣ Workshop/Orientation course on “Laboratory methods in gene transfer and gene expression”
(MICR, 536), at Loma Linda University, Loma Linda, CA, USA (18 th June – 29 th June 2007).
‣ “Proteomics: Principles and Methods”- Trac 25, A Biotechnology (BioTrac) workshop/training
course conducted by foundation for advanced education in the sciences (FAES) at National
Institute of Health (NIH), in Bethesda, MD, USA. (1 st oct to 5 th Oct 2007).
‣ International Conference on Molecules to Materials-06, SLIET, Longowal, Panjab.

‣ 88 th Indian Science Congress Association (IARI- New Delhi) – 2001.
‣ 89 th Indian Science Congress Association (Lucknow University) – 2002.
‣ Workshop on "Recent Trends in Genomics and Proteomics" organized by Bioinformatics
centre, H.P. Universitv. Shimla – 2002.
‣ 90 th Indian Science Congress Association (Bangalore University) – 2003.
‣ 91 st Indian Science Congress Association (Panjab University) – 2004
‣ 92 nd Indian Science Congress Association (Nirma University, Ahemdabad) - 2005
‣ 93 rd Indian Science Congress Association (Acharya Ranga University, Hyderabad-2006)
‣ 94 th Indian Sceince Congress Association (Anna Malai University, Tamil Nadu -2007, India)
‣ "Patent Awareness Workshop" organized by Punjab State Council for Science and Technology
and Biochemistry Dept., K.U.K.
‣ Seminar-cum-training on "Electron Microscope" organized by SAIF (P.U. Chandigarh) and
Zoology Dept., K.U.K.
‣ Seminar on "Emerging Trends in Biotechnology" organized by Sartorius group (German) and
Biotechnology Dept., K.UK
‣ Workshop on "Trends in Computational Biology" organized by Bioinformatics centre, NDRI,
Karnal, INDIA, 18 th Oct – 20 th Oct 2005.
‣ Workshop on "Advances in Bio-informatics" organized by Bioinformatics Centre, at GNDU,
Amritsar, Panjab from 23 rd Feb – 24 th Feb 2006.
Invited lectures:
“A Molecular Tool for the Authentication of Medicinal Plants: DNA Fingerprinting”, on the
occasion of Reorientation Training Program (Sanctioned by Dept. of AYUSH, M/o Health & F W,
Govt. of India) at Regional Research Institute (Ay) Kothrud, Pune. (12.12.2009).
“DNA fingerprinting: A molecular tool for the authentication of herbal plants”, on the occasion of
Reorientation Training Program (Sanctioned by Dept. of AYUSH, M/o Health & F W, Govt. of
India) at Regional Research Institute (Ay) Kothrud, Pune. (27.02.2010).
Lecture delivered on topic entitled, “Overview of Carbohydrate Metabolism” to the PG students
of Biochemistry/Biotechnology/Biosciences departments at Dolphin (PG) Institute of Bio-Medical
& Natural Sciences, Dehradun, Uttarakhand. (12.08.2010)
Lecture delivered on topic entitled, “Purification of protein and Criteria of their Purity” to the PG
students of Biochemistry/Biotechnology/Biosciences departments at Dolphin (PG) Institute of
Bio-Medical & Natural Sciences, Dehradun, Uttarakhand. (29.03.2011)
Hands on Experience with the Techniques:
Electrophoresis, Column Chromatography (Gel filtration, Affinity and ion exchange), ELISA,
Isoelectrofocusing, Western Blotting, Co-immunoprecipitation, GST-, His-pull down assay,
Double immunoflourescence, Histochemistry, Electrophoretic mobility shift assay (EMSA),
muscle cell culture, denervation, cardiotoxin, and skeletal muscle cell culture etc.
Teaching expertise:
Protein Biochemistry, Enzyme kinetics and Metabolism, Molecular Biology & Immunology