SRMP Lab Skills: Molecular Biology Curriculum - American Museum ...

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Science Research Mentoring Program MOLECULAR BIOLOGY Session One: Laboratory Math learning objectives Students should understand the common SI units and prefixes used in the lab, be able to convert between SI units and solve basic dilution problems, and do basic molarity calculations. key topics • SI units and prefixes • Solutions in molecular biology • Dilutions in molecular biology • Molarity Class outline time TOPIC description 15 minutes 10 minutes 10 minutes 15 minutes 20 minutes 15 minutes 20 minutes Lab Safety Lecture: SI SI: Practice Lecture: Dilutions and Stock Solutions Dilutions and Stock Solutions: Practice Lecture: Molarity Molarity: Practice Review lab safety rules. Explore why scientists use SI units, commonly used prefixes in molecular biology, and conversions. Students complete conversion worksheet; share answers. Units of concentration (w/v, v/v, M, X), calculating dilution volumes and concentrations using C1V1=C2V2. Students complete dilution worksheets; share answers. Definition of molarity; difference between moles and molarity; calculating molarity. Students complete molarity worksheet; share answers. materials • Worksheets • Calculators PREPARATION None required homework None © 2013 American Museum of Natural History. All Rights Reserved. 2
Science Research Mentoring Program MOLECULAR BIOLOGY Session One: Laboratory Math: HANDOUT Lab Safety Rules 1. Conduct yourself responsibly at all times. 2. Never work alone. No student may work in the science classroom without a teacher present. 3. Do not eat, drink, or chew gum. Do not use laboratory glassware to hold food or beverages. 4. Hang up all backpacks and coats properly; never hang them on the back of your chair. Work areas and floor space should be kept clear and tidy. 5. Wear safety goggles whenever using chemicals, heat, or glassware. 6. Don’t wear contact lenses. 7. Dress properly. Long hair, and dangling jewelry and clothing are hazards in a laboratory setting. Long hair must be tied back. 8. Shoes must cover the foot. No sandals. 9. Examine glassware before each use. Never use chipped, cracked, or dirty glassware. Observe a liquid by placing the glassware on a table; never hold it overhead. 10. Remember that heated glassware remains very hot for a long time. Set it in a designated place to cool — not directly on the laboratory desk — and always on an insulated pad. Allow plenty of time for hot apparatus to cool, and handle with tongs or gloves if necessary. 11. Never look into a container that is being heated. Do not immerse hot glassware in cold water, as it may shatter. © 2013 American Museum of Natural History. All Rights Reserved. 3
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SRMP Lab Skills: Molecular Biology Curriculum - American Museum ...

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