SRMP Lab Skills: Molecular Biology Curriculum - American Museum ...
SRMP Lab Skills: Molecular Biology Curriculum - American Museum ...
SRMP Lab Skills: Molecular Biology Curriculum - American Museum ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Science Research Mentoring Program<br />
MOLECULAR BIOLOGY<br />
Session Three: DNA Extraction<br />
learning objectives<br />
Students should understand the principles of DNA purification, be able to pipette accurately,<br />
and use a centrifuge properly.<br />
key topics<br />
• DNA extraction/purification<br />
Class outline<br />
time<br />
TOPIC<br />
description<br />
30 minutes<br />
10 minutes<br />
Wrap-up of Pipetting Accuracy<br />
<strong>Lab</strong>oratory Notebook Review<br />
Go over results from pipetting accuracy test.<br />
Discuss what should be recorded in a laboratory<br />
notebook, the purpose of a lab notebook.<br />
1 hour<br />
15 minutes<br />
DNA Extraction<br />
Give students protocol for Chelex cheek cell DNA<br />
extraction, go over as a class, let students perform on<br />
their own. Make sure to do the first spin as a class, and<br />
make sure they resuspend the Chelex prior to pipetting.<br />
Store extracted DNA in the freezer.<br />
15 minutes<br />
For Next Session:<br />
PCR Reaction Worksheet<br />
Have students start the PCR reaction worksheet; finish as<br />
homework.<br />
materials<br />
PREPARATION<br />
• Chelex<br />
Turn on heat block to 99C<br />
• 1.5 ml tubes<br />
• Micropipetters<br />
• pipet tips<br />
HOMEWORK<br />
Finish PCR reaction worksheet<br />
• microfuge<br />
• gloves<br />
• heat block<br />
© 2013 <strong>American</strong> <strong>Museum</strong> of Natural History. All Rights Reserved. 12
Change language