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In touch with life - Eppendorf

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272<br />

Electroporation and electrofusion<br />

Electroporation and electrofusion buffer system<br />

Product features<br />

‡‡<br />

The hypoosmolar buffer system swells the cell for<br />

easier membrane penetration<br />

‡‡<br />

The ion composition is adapted to the inner cell<br />

environment, stabilizing the Na + /K + gradient across<br />

the cell membrane<br />

Description<br />

The electroporation and electrofusion buffer systems used <strong>with</strong><br />

the Eporator ® and Multiporator ® contribute towards increased<br />

efficiency. The hypoosmolar buffer stretches the cell membrane<br />

and relaxes the cell skeleton, thus simplifying fusion in the<br />

electrical field. Cell-damaging influences, such as long pulse<br />

and high voltages are reduced.<br />

The <strong>Eppendorf</strong> ® electroporation buffer should be used for the<br />

electroporation of eukaryotic cells (<strong>with</strong> the exception of yeasts<br />

and some microorganisms and plants). These buffers are tested<br />

for sterility and the absence of mycoplasms and endotoxins.<br />

Ordering information<br />

Description Catalog No. List USD<br />

Electroporation buffer system<br />

Hypoosmolar, sterile, 100 mL 940002001 $52.00<br />

Isoosmolar, sterile, 100 mL 940002109 52.00<br />

Electrofusion buffer system<br />

Hypoosmolar, sterile, 100 mL 940002150 52.00<br />

Isoosmolar, sterile, 100 mL 940002206 52.00<br />

Electroporation cuvettes<br />

Product features<br />

8 | Consumables | Cell Technology<br />

‡‡<br />

Plastic cuvette <strong>with</strong> aluminum electrodes<br />

‡‡<br />

Three sizes <strong>with</strong> 100 µL, 400 µL and 800 µL filling<br />

capacity (1 mm, 2 mm and 4 mm gap width)<br />

‡‡<br />

<strong>In</strong>dividually wrapped and gamma-irradiated to<br />

ensure sterility<br />

‡‡<br />

Design ensures correct positioning in the cuvette holder<br />

‡‡<br />

Gap width marked on the side of every cuvette to<br />

simplify identification<br />

‡‡<br />

Frosted area permits easy labeling<br />

Ordering information<br />

Description Catalog No. List USD<br />

Electroporation cuvettes, gap width 1 mm, 100 µL, sterile, 50 pcs. 940001005 $190.00<br />

Electroporation cuvettes, gap width 2 mm, 400 µL, sterile, 50 pcs. 940001013 190.00<br />

Electroporation cuvettes, gap width 4 mm, 800 µL, sterile, 50 pcs. 940001021 190.00<br />

Cuvette stand, for 16 electroporation cuvettes 940001102 30.00<br />

Order from your laboratory supply dealer.<br />

Product appearance, specifications, and/or prices are subject to change <strong>with</strong>out notice.

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