In touch with life - Eppendorf

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Electroporation and electrofusion 271 Multiporator® with module for bacteria and yeasts Product features ‡‡ Programmable voltage and optimized, fixed pulse times ‡‡ Preadjusted voltages for the most frequent applications ‡‡ Electronic safety circuit to prevent arcing Applications ‡‡ Electroporation of bacteria, yeasts and other microorganisms Technical specifications Bacteria and yeast module Pulse voltage 200–2,500 V Pulse form Exponentially diminishing Time constant 5 ms (nominal) Resistance 600 Ω Capacitor 10 µF Special feature Electronic safety switch for eliminating short-circuits Protocols are available at www.eppendorfna.com/bacteria Ordering information Description Catalog No. List USD Multiporator For eukaryotic cells 940000505 $ 5,580.00 For eukaryotic cells, bacteria and yeasts 940000602 6,700.00 For eukaryotic cells and cell fusion, with 1 Helix fusion chamber and 1 Micro fusion chamber 940000700 11,590.00 For eukaryotic cells, bacteria, yeasts and cell fusion, with 1 Helix fusion chamber and 940000807 13,470.00 1 Micro fusion chamber Insert For connecting external electrodes (electrofusion/electroporation) 940004209 830.00 Electroporation Buffer System Hypoosmolar, sterile, 100 mL 940002001 52.00 Isoosmolar, sterile, 100 mL 940002109 52.00 Electrofusion Buffer System Hypoosmolar, sterile, 100 mL 940002150 52.00 Isoosmolar, sterile, 100 mL 940002206 52.00 Helix fusion chamber For cell fusion 940001200 1,200.00 Micro fusion chamber Gap width 0.2 mm 940001251 430.00 Gap width 0.5 mm 940001234 470.00 Stand For 10 Helix fusion chambers 940001218 110.00 8 | Instruments | Cell Technology In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: info@eppendorf.com · www.eppendorf.com
272 Electroporation and electrofusion Electroporation and electrofusion buffer system Product features ‡‡ The hypoosmolar buffer system swells the cell for easier membrane penetration ‡‡ The ion composition is adapted to the inner cell environment, stabilizing the Na + /K + gradient across the cell membrane Description The electroporation and electrofusion buffer systems used with the Eporator ® and Multiporator ® contribute towards increased efficiency. The hypoosmolar buffer stretches the cell membrane and relaxes the cell skeleton, thus simplifying fusion in the electrical field. Cell-damaging influences, such as long pulse and high voltages are reduced. The Eppendorf ® electroporation buffer should be used for the electroporation of eukaryotic cells (with the exception of yeasts and some microorganisms and plants). These buffers are tested for sterility and the absence of mycoplasms and endotoxins. Ordering information Description Catalog No. List USD Electroporation buffer system Hypoosmolar, sterile, 100 mL 940002001 $52.00 Isoosmolar, sterile, 100 mL 940002109 52.00 Electrofusion buffer system Hypoosmolar, sterile, 100 mL 940002150 52.00 Isoosmolar, sterile, 100 mL 940002206 52.00 Electroporation cuvettes Product features 8 | Consumables | Cell Technology ‡‡ Plastic cuvette with aluminum electrodes ‡‡ Three sizes with 100 µL, 400 µL and 800 µL filling capacity (1 mm, 2 mm and 4 mm gap width) ‡‡ Individually wrapped and gamma-irradiated to ensure sterility ‡‡ Design ensures correct positioning in the cuvette holder ‡‡ Gap width marked on the side of every cuvette to simplify identification ‡‡ Frosted area permits easy labeling Ordering information Description Catalog No. List USD Electroporation cuvettes, gap width 1 mm, 100 µL, sterile, 50 pcs. 940001005 $190.00 Electroporation cuvettes, gap width 2 mm, 400 µL, sterile, 50 pcs. 940001013 190.00 Electroporation cuvettes, gap width 4 mm, 800 µL, sterile, 50 pcs. 940001021 190.00 Cuvette stand, for 16 electroporation cuvettes 940001102 30.00 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice.
Page 1 and 2: In touch with life
Page 3 and 4: 266 Cell technology Summary of appl
Page 5 and 6: 268 Electroporation and electrofusi
Page 7: 270 Electroporation and electrofusi
Page 11 and 12: 274 Microinjection and manipulation
Page 23 and 24: 286 Capillaries for microinjection
Page 25 and 26: 288 Capillaries for microinjection
Page 27 and 28: 290 Capillaries for microdissection
Page 29: Support and Services Directory Cont

In touch with life - Eppendorf

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