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Microinjection and manipulation of cells 281 Microinjection overview For more than 30 years now, researchers have been introducing foreign molecules, such as DNA, RNA, proteins, toxins and dyes, into living cells using a technique called microinjection. This technique offers many advantages, including the targeting of individual cells and/or subcellular structures as well as the ability to reproducibly inject a defined amount of solution into these targets. Applications include transfection and analysis experiments, signal transduction, cytotoxicity tests and more. In addition to an ultra-precise microinjection device, accurate and reproducible microinjection also requires ultra-fine glass capillaries—they allow the targeting of very specific cell areas, such as the nucleus and the cytoplasm. For optimal efficiency and viability, microinjection systems (consisting of microinjectors, micromanpulators and capillaries) must be accurate and precise, perform fast yet smooth movement with gentle yet effective penetration, have a userfriendly interface and a large operating range and, finally, be solidly constructed for complete reliability. Eppendorf ® Cell Technology systems have always met these requirements—in fact, we continually set new standards for performance, efficiency, reproducibility and reliability: For example, the FemtoJet ® and FemtoJet express microinjectors feature a broad range of pressure settings for precise and accurate microinjection with solutions as small as femtoliters (10 -9 µL) on up to 1 µL and above. FemtoJet For injecting small to intermediate volumes (up to 100 pL), the original FemtoJet is perfectly suited, featuring a wide range of functionality, simple operation and built-in compressor to independently deliver the required pressure. It is ideal for injecting aqueous solutions into adherent cells (and makes the ideal partner with our InjectMan ® NI 2 and 5179, as well as Model 5171) and suspension cells (with TransferMan ® NK 2). The FemtoJet forms the perfect system with Eppendorf micromanipulators, and it integrates just as easily with non-Eppendorf microinjection systems. Applications: FemtoJet ‡‡ Semiautomatic injection into adherent cells with InjectMan NI 2 (and older models 5171 and InjectMan 5179) ‡‡ Suspension cell injection with TransferMan NK 2 ‡‡ Pronuclear injections into mouse embryos ‡‡ Serial injection volumes from femtoliter up to 100 pL FemtoJet express If your research demands injecting volumes greater than 100 pL and/or longer series at higher pressures—increasingly seen in functional genomics and developmental biology applications— the FemtoJet express with its external pressure supply delivers the precise and continuous pressure required. Featuring the same quality design and compatibility features as our original FemtoJet, the express model is your heavy duty device for those more demanding applications. Applications: FemtoJet express ‡‡ Microinjection into small organisms or embryos at early developmental stages: e.g., Drosophila melanogaster, Xenopus laevis, Caenorhabditis elegans, zebrafish, medaka, etc. ‡‡ Pronuclear injections into mouse embryos ‡‡ Serial injection volumes from 100 pL up to 1 µL 8 | Instruments | Cell Technology In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: info@eppendorf.com · www.eppendorf.com
282 Microinjection and manipulation of cells FemtoJet® and FemtoJet express General product features ‡‡ Programmable injection ensures reproducibility ‡‡ Injection volumes range from femtoliters to microliters ‡‡ User-friendly interface with large LCD for easy operation ‡‡ Additional menu-controlled functions (e.g. for bubble pressure test) 1 ‡‡ Inject and clean functions triggered via control button on instrument, supplied mouse or optional foot control ‡‡ Electronic linkage to InjectMan ® NI 2 2 and older models 5171 and InjectMan 5179 ‡‡ Easily combined with all common micromanipulation systems Technical specifications Description FemtoJet FemtoJet express Pressure supply Injection time (Ti) Injection pressure (Ti) Compensation pressure (Pc) Rinsing pressure Dimensions (W x H x D) Integrated compressor 1 Schnorft et al. 1994, Exp. Cell Res.210 2 3 U.S. Patent 5,114,454 U.S. Patent 4,336,244 External pressure source 00–99.9 s can be set in increments of 0.1 s 0; 5 hPa–max. 6,000 hPa (87 psi); can be set in increments of 1 hPa 0; 5 hPa–max. 6,000 hPa (87 psi); can be set in increments of 1 hPa Max. 7,000 hPa (102 psi) 8.6 x 11.0 x 6.7 in 22 x 28 x 17 cm Weight 13.7 lb/6.2 kg 10.3 lb/4.7 kg Additional product features FemtoJet 3 ‡‡ Independent pressure supply with built-in compressor FemtoJet express ‡‡ External pressure supply delivers higher pressure at longer intervals for long series experiments, larger volumes and/or larger targets Typical volumes for microinjection into: Nucleus of adherent cells Cytoplasm of adherent cells Pronucleus of mouse oocytes Caenorhabditis elegans Early fish embryos Xenopus laevis oocytes FemtoJet FemtoJet express Volume (liter, logarithmic scale) 10 -15 10 -12 10 -9 10 -6 Unit femtoliter picoliter nanoliter microliter FemtoJet is suitable for applications that require lower volume ranges; FemtoJet express is better suited for applications that require higher volume ranges. Both feature the same injection time and pressure parameters. 8 | Instruments | Cell Technology Ordering information Description Catalog No. List USD FemtoJet, programmable microinjector with integrated pressure supply 920010504 $9,520.00 FemtoJet express, programmable microinjector with external pressure supply 920010521 8,340.00 Foot control, for triggering inject function 920005098 260.00 Universal capillary holder, for Femtotips, Femtotips II or common microcapillaries 920007392 330.00 2 positioning aids, for universal capillary holder 920005829 110.00 Grip heads for universal capillary holders Grip head 0, for microcapillaries*, outer diameter 1.0–1.1 mm, set of 2 920007414 140.00 Grip head 1, for microcapillaries, outer diameter 1.2–1.3 mm, set of 2 920007708 140.00 Grip head 2, for microcapillaries, outer diameter 1.4–1.5 mm, set of 2 920007716 140.00 Grip head 3, for microcapillaries, outer diameter 0.7–0.9 mm, set of 2 920007406 140.00 Spare parts Service kit, includes O-ring set plus different adapters 920005888 130.00 Injection tube, 2 m 920007431 110.00 Pressure tube for FemtoJet express, 2.5 m, includes coupling G. 1/4 in 920011993 130.00 * Pressure tube for FemtoJet express is included with the purchase of the FemtoJet express Order from your authorized Eppendorf dealer. Product appearance, specifications, and/or prices are subject to change without notice.
Page 1 and 2: In touch with life
Page 3 and 4: 266 Cell technology Summary of appl
Page 5 and 6: 268 Electroporation and electrofusi
Page 11 and 12: 274 Microinjection and manipulation
Page 17: 280 Microinjection and manipulation
Page 23 and 24: 286 Capillaries for microinjection
Page 25 and 26: 288 Capillaries for microinjection
Page 27 and 28: 290 Capillaries for microdissection
Page 29: Support and Services Directory Cont

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