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Microinjection and manipulation of cells
277
InjectMan® NI 2
Semiautomatic microinjection into adherent cells
As previously described, the electronic coupling of the
InjectMan NI 2 1 with the FemtoJet ® (page 281) makes
microinjection into adherent cells possible. This
semiautomatic sequence of manipulation and injection
occurs first by programming the injection parameters
on the FemtoJet and then establishing the injection level
(Z-limit) by pressing a button on the InjectMan NI 2.
By activating the joystick key, the patented axial injection
movement 2 of the capillary (A, B) and the programmed pressure
increase can be simultaneously triggered on the FemtoJet.
Following the preset injection time, the pressure decreases in
the capillary and is triggered simultaneously with the movement
of the capillary back to its starting position (C). Thus, the
capillary tip serves as a “pointer” for the target location in the
cell, and the exact axial injection movement secures the highest
survival rate*. With the help of this technology, it is now possible
to process more than 40 cells per minute simply, efficiently
and reproducibly.
search level
injection level
carrier
Microinjection
A
B
C
1
U.S. Patent 5,114,854
2
U.S. Patent 4,836,244
* Peloquin, et al. BioTechniques. 1997;22:496-499.
Technical specifications
Ordering information
Control
Drive
Resolution
Max. movement
(each axis)
Angle of inclination
Max. speed of tool
Power supply,
Input:
Output:
Dimensions (W x D x H)
Y/Z module
Dimensions (W x D x H)
X module
Dimensions (W x D x H)
Control panel
Weight Module unit,
complete
Via central joystick;
dynamic kinetics
stepper motor
approx. 40 nm per micro step
approx. 20 mm
30°–60° to object table
7,500 µm/s
120–240 V AC, 50–60 Hz, < 50 W
15–18 V DC, 25 W
4.72 x 3.94 x 2.68 in/
12.0 x 10.0 x 6.8 cm
4.72 x 2.36 x 1.97 in/
12.0 x 6.0 x 5.0 cm
10.63 x 8.07 x 6.30 in/
27.0 x 20.5 x 16.0 cm
3.1 lb/1.4 kg
Description Catalog No. List USD
InjectMan NI 2
920000029 $19,800.00
manipulator for semiautomatic
microinjection into adherent cells
Connecting cable,
920005853 200.00
for InjectMan NI 2 and
Transjector 5246 or
Microinjector 5242
Connecting cable,
920005845 100.00
for InjectMan NI 2, FemtoJet
and FemtoJet express
Foot pedal control,
920005799 160.00
for InjectMan NI 2
Interface cable for PC 920005837 190.00
Microscope adapters,
see page 280
8 | Instruments | Cell Technology
Weight Control panel
with power supply
6 lb/2.7 kg
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com