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In touch with life - Eppendorf

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276<br />

Microinjection and manipulation of cells<br />

<strong>In</strong>jectMan® NI 2<br />

Workstation for microinjection into adherent cells:<br />

<strong>In</strong>jectMan NI 2 and FemtoJet ® microinjection workstation,<br />

shown <strong>with</strong> Zeiss Axio ® Observer microscope<br />

Workstation for microinjection into fish embryos:<br />

<strong>In</strong>jectMan NI 2, stereomicroscope and FemtoJet express<br />

8 | <strong>In</strong>struments | Cell Technology<br />

Product features<br />

‡‡<br />

<strong>In</strong>tegrated coarse and fine manipulator <strong>with</strong> dynamic<br />

control via central joystick<br />

‡‡<br />

Work range: 20 mm per axis<br />

‡‡<br />

Automated axial injection movement<br />

‡‡<br />

Programmable Z-axis limit at defined injection level<br />

avoids capillary breakage<br />

‡‡<br />

Automated Home function for rapid capillary exchange<br />

‡‡<br />

Adjustable injection angle and injection speed for<br />

different complex injection processes<br />

‡‡<br />

Axial mounting of Z module<br />

‡‡<br />

Special inject mode for applications in<br />

developmental biology<br />

‡‡<br />

Fine adjustment of work speed made easy<br />

<strong>with</strong> positioning wheel<br />

‡‡<br />

Menu-controlled programming via display<br />

‡‡<br />

Save and download user profiles<br />

‡‡<br />

External control for automatic processes*<br />

‡‡<br />

Can be adapted to all common microscopes<br />

* The connection of the PC is made via a zero modem cable <strong>with</strong> an elbow plug (SubD9, female,<br />

e.g. PC cable update, order no. 920005837<br />

Data rate: 19,200 Bit/s<br />

The RS 232 data port must only be connected to devices conforming to the IEC 950/EN 60950<br />

(UL1950) standards.<br />

Applications<br />

‡‡<br />

Semiautomatic microinjection into adherent cells<br />

‡‡<br />

Serial microinjection into fish larvae, insect embryos, etc.<br />

Description<br />

A menu-controlled, programmable micromanipulator, the<br />

<strong>In</strong>jectMan NI 2 1 is especially suitable for microinjection into<br />

adherent cells. The electronic connection of the <strong>In</strong>jectMan NI 2<br />

and the FemtoJet 2 or FemtoJet express (page 281) ensures a<br />

very rapid and reproducible microinjection. The semiautomatic<br />

microinjection allows a coordinated process of manipulation and<br />

injection: first, the injection parameter is set on the FemtoJet;<br />

second, the injection level is fixed via pressing a button on the<br />

<strong>In</strong>jectMan NI 2. The exact axial injection movement ensures<br />

a minimum mortality rate, and its high speed facilitates the<br />

penetration of rigid structures.<br />

1<br />

U.S. Patent 5,114,854<br />

2<br />

U.S. Patent 4,836,244<br />

Order from your authorized <strong>Eppendorf</strong> dealer.<br />

Product appearance, specifications, and/or prices are subject to change <strong>with</strong>out notice.

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