SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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How luminescence is detected • Photomultiplier tube (PMT) converts incoming photons into electrons • Incoming photon strikes photocathode → generates electron • Electron flows through a series of electron multipliers (dynodes) to the anode • Current flowing from anode ∝ number of photons at the photocathode • Amount of amplification a PMT can produce depends on (a) number of dynodes and (b) voltage applied to it 4 Source: http://micro.magnet.fsu.edu/primer/digitalimaging/concepts/photomultipliers.html
SpectraMax ® L luminometer: Four PMT settings • Analog Only: Ideal for very bright signals above 1.6 x 10 7 RLU (low PMT voltage) • Photon Counting: Ideal for very dim and medium signals below 25 x 10 6 RLU (digital) • AutoRange: Extends range of Photon Counting mode by adding Analog (high PMT voltage) • MaxRange: Combines AutoRange and Analog Only modes o Captures entire dynamic range o 10 to 10 9 RLU AutoRange MaxRange 5
Page 1 and 2: Increase your luminescence throughp
Page 3: Introducing the SpectraMax ® L lum
Page 7 and 8: SpectraMax ® L luminometer - confi
Page 9 and 10: AutoWash function Upon instrument s
Page 11 and 12: Applications for SpectraMax ® L sy
Page 13 and 14: Moving on… • Introducing the Sp
Page 15 and 16: Advantages of aequorin assays • L
Page 17 and 18: Aequorin assays on SpectraMax ® L
Page 19 and 20: AequoZen M 5 cell line: agonists Co
Page 21 and 22: AequoZen H 1 cell line - agonists C
Page 23 and 24: SpectraMax ® L & FLIPR TETRA® sys
Page 25 and 26: Acknowledgments • Carole Crittend

SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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