SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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AequoZen frozen cells (PerkinElmer) • Cryopreserved, γ-irradiated cell lines • Good for single use, cannot be propagated • CHO-K1 cell lines expressing GPCR o Histamine H 1 (stronger response) o Muscarinic M 5 (weaker response) 18
AequoZen M 5 cell line: agonists Concentration-response curves from SpectraMax ® L system EC 50 from product insert = 16.2 nM EC 50 from product insert = 25.7 nM 19
Page 1 and 2: Increase your luminescence throughp
Page 3 and 4: Introducing the SpectraMax ® L lum
Page 5 and 6: SpectraMax ® L luminometer: Four P
Page 7 and 8: SpectraMax ® L luminometer - confi
Page 9 and 10: AutoWash function Upon instrument s
Page 11 and 12: Applications for SpectraMax ® L sy
Page 13 and 14: Moving on… • Introducing the Sp
Page 15 and 16: Advantages of aequorin assays • L
Page 17: Aequorin assays on SpectraMax ® L
Page 21 and 22: AequoZen H 1 cell line - agonists C
Page 23 and 24: SpectraMax ® L & FLIPR TETRA® sys
Page 25 and 26: Acknowledgments • Carole Crittend

SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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