SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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Disadvantages • Requires reconstitution with coelenterazine o Similar to dye loading, but from 4 hours to overnight • Aequorin consumption o No long-term recording or <strong>multi</strong>ple additions • Possible EC 50 /IC 50 shift compared to other methods • Not all GPCR assays are amenable to aequorin o GPCRs that do not signal via Ca ++ 16
Aequorin assays on SpectraMax ® L system • Preconfigured protocol in SoftMax ® Pro software • Automatic data reduction and analysis • 4-parameter curve fit 17
Page 1 and 2: Increase your luminescence throughp
Page 3 and 4: Introducing the SpectraMax ® L lum
Page 5 and 6: SpectraMax ® L luminometer: Four P
Page 7 and 8: SpectraMax ® L luminometer - confi
Page 9 and 10: AutoWash function Upon instrument s
Page 11 and 12: Applications for SpectraMax ® L sy
Page 13 and 14: Moving on… • Introducing the Sp
Page 15: Advantages of aequorin assays • L
Page 19 and 20: AequoZen M 5 cell line: agonists Co
Page 21 and 22: AequoZen H 1 cell line - agonists C
Page 23 and 24: SpectraMax ® L & FLIPR TETRA® sys
Page 25 and 26: Acknowledgments • Carole Crittend

SpectraMaxÂ® L multi-detector microplate luminometer - Molecular ...

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