SpectraMax® L multi-detector microplate luminometer - Molecular ...

Advantages of aequorin assays
• Luminescent signal reduces problems of auto-fluorescence,
compound interference
• Targeting photoprotein expression to specific parts of cell allows
more target-specific signal resolution
• Wide dynamic range (10 -7 to 10 -4 M) covers physiological Ca ++
signals
• Low calcium buffering effect compared to fluorescent reagents
• Expression is non-toxic
• Reduced cost (10x less) compared to fluorescence or luciferase
assays
• Cells can be delivered in suspension*
o Reduces cost
15
o Increases throughput *These assay methods are also described in the literature, e.g. in Boie et al., Eur. J.
Pharmacol., 340(2-3):227-241 (1997), and in United States Patent 6,872,538 and
European Patent 1,145,002. Users interested in the patented methods may wish to
consult legal counsel in evaluating these patents.