SpectraMax® L multi-detector microplate luminometer - Molecular ...
SpectraMax® L multi-detector microplate luminometer - Molecular ...
SpectraMax® L multi-detector microplate luminometer - Molecular ...
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Aequorin overview<br />
• Photoprotein originating from the jellyfish Aequorea victoria<br />
• Aequorin binds Ca ++ → oxidizes coelenterazine → CO 2 + light<br />
• Aequorin is used to detect GPCR signaling via Ca ++ in cells<br />
• Reaction is very fast (10-30 seconds)<br />
• Assay is read on <strong>luminometer</strong> equipped with injector or pipettor<br />
GPCR<br />
Agonist<br />
α β γ<br />
PLC<br />
Apoaequorin<br />
Coelenterazine<br />
IPs<br />
Aequorin<br />
+<br />
Ca ++<br />
14<br />
Flash lumi
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