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A manual of rice seed health testing - IRRI books - International Rice ...

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solid pointed end. The tube and<br />

sleeve have open slots in their walls.<br />

When the slots are alined, <strong>seed</strong>s<br />

drop into the cavity <strong>of</strong> the tube. To<br />

close the slots, turn the tube. Tube<br />

length and diameter depend on the<br />

size <strong>of</strong> the container and kind <strong>of</strong> the<br />

<strong>seed</strong> to be sampled.<br />

The trier can be used horizontally<br />

for vertically. A vertical trier should<br />

be partitioned to assure even sampling<br />

through the layers.<br />

Insert the closed trier diagonally<br />

into the bag or container. Alter insertion,<br />

agitate it gently, and open and<br />

close it several times to ensure filling.<br />

Finally, close carefully before<br />

drawing out the sample.<br />

b) The Nobbe trier (Fig. 4.2a) is<br />

suitable for sampling <strong>seed</strong> in bags<br />

but not in bulk. It is constructed in<br />

varying dimensions depending on<br />

the kinds <strong>of</strong> <strong>seed</strong>s to be sampled.<br />

The Nobbe trier is a pointed tube,<br />

long enough to reach the center <strong>of</strong><br />

the bag, with an oval hole near the<br />

pointed end, The instrument should<br />

be approximately 500 mm long, including<br />

the handle (about 100 mm<br />

long) and point (about 60 mm), leaving<br />

about 340 mm to penetrate the<br />

bag. This should reach the center <strong>of</strong><br />

all types <strong>of</strong> bags. For cereals, the internal<br />

diameter <strong>of</strong> the tube should be<br />

about 14 mm. For clovers and similar<br />

<strong>seed</strong>s, 10 mm is sufficient.<br />

Insert the trier gently inlo the bag<br />

(Fig. 4.2b), pointing upward at an<br />

angle <strong>of</strong> about 30° to the horizontal,<br />

with the hole facing downward, until<br />

it reaches the center <strong>of</strong> the bag.<br />

Rotate the trier 180°, bringing the<br />

hole on top. Withdraw the trier with<br />

decreasing speed so the quantity <strong>of</strong><br />

<strong>seed</strong> obtained from successive locations<br />

increases progressively from<br />

the center to the side <strong>of</strong> the bag. If,<br />

howerver, the trier is long enough to<br />

penetrate to the opposite side <strong>of</strong> the<br />

bag, withdraw it at a relatively constant<br />

speed. During withdrawal, the<br />

trier should be gently agitated to<br />

maintain an even flow <strong>of</strong> <strong>seed</strong>. The<br />

more polished the inner surface <strong>of</strong><br />

the trier, the more freely the <strong>seed</strong><br />

will flow.<br />

To sample the bottoms <strong>of</strong> standing<br />

bags, raise them <strong>of</strong>f the floor and<br />

place them on top <strong>of</strong> other bags. The<br />

holes that the trier made in the bags<br />

may be closed by pushing the weave<br />

back together.<br />

c) Sampling by hand is difficult,<br />

and should not be used for chaffy<br />

<strong>seed</strong>s like <strong>rice</strong>.<br />

3. Taking primary samples (Rule<br />

2.6.4)<br />

Primary samples <strong>of</strong> approxirnately<br />

equal size shall be taken from each<br />

container sampled. When the lot is<br />

in containers (including bags), the<br />

containers to be sampled shall be selected<br />

at random throughout the lot<br />

and primary samples drawn from<br />

top, middle, or bottom portions <strong>of</strong><br />

the container but not necessarily<br />

from more than one position in any<br />

container. When the <strong>seed</strong> is in bulk<br />

or in large containers, the primary<br />

samples shall be drawn from random<br />

positions or depths.<br />

4. Obtaining the composite sample<br />

(Rule 2.6.5)<br />

If the primary samples appear uniform,<br />

they shall be combined to form<br />

the composite sample.<br />

4.1 A sleeve-type<br />

trier (grain probe).<br />

4.2 a. Stick triers<br />

(Nobbe trier).<br />

b. Inserting a stick<br />

trier in a sack <strong>of</strong> <strong>rice</strong><br />

to draw <strong>seed</strong>s.<br />

20 <strong>Rice</strong> <strong>seed</strong> <strong>health</strong> <strong>testing</strong> <strong>manual</strong>

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