A manual of rice seed health testing - IRRI books - International Rice ...

3.2 Dry seed
inspection using a
magnifying lens.
3.3 Dry seed
inspection using a
stereobinocular
microscope.
Data from the tests should be recorded
on appropriate recording
sheets and signed by the responsible
analyst. The names of fungi
should be recorded according to the
state they are found during testing.
If conidia are found, the conidial
epithet is recorded; if the perfect
stage is detected, the perfect stage
epithet is recorded; if both are
present, both epithets are recorded.
For uniformity in recording, the
currently accepted epithet should
be used. (See Appendix 1 for sample
recording sheets for dry seed
inspection, washing test, blotter and
agar plate tests, and for
Aphelenchoides besseyi.).
Table 3.2. Methods and equipment used in rice seed health testing.
Method
Direct method
For sclerotia,
smut, malformed
and moldy seeds
Tilletia barclayana
contamination
Washing test
Aphelenchoides
besseyi sedimentation
test
Indirect method
Blotter test
Agar test for
fungi and
bacteria
Test tube agar
growing-on test
Inoculation
Other methods
Serological
test
Preparation
procedures
Shake by hand
or use automatic
shaker; then
centrifuge at low
speed
Use modified
Baermann funnel
In NUV incubation
21 °C, with timer
12 h NUV light/
12 h darkness
Seed surface
sterilization in
table top
incubators (28 °C)
In growth chamber + b
(28 °C) 12 h light/
day or on laboratory
table top
near window
In growth chamber +
(28 °C) 12 h light
or in glasshouse
As required
Detection/identificatlon tools
Hand Stereobinocular Compound Others
lens microscope microscope
+
+ +
+ +
+ Haemocytometer
Tally counter
+ + Tally counter
+ + Freezer for deep
+ a + a freezing method
Laminar flow
cabinet
Sterile sand/
soil, light
a To verify spores. b To magnify developing lesions. Equipment 15