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A Manual of Rice Seed Health Testing Edited by T.W. Mew and J.K. Misra 1994 IRRI INTERNATIONAL RICE RESEARCH INSTITUTE Los Baños, Laguna, Philippines Mail Address: P.O.Box 933, 1099 Manila, Philippines
The International Rice Research Institute (IRRI) was established in 1960 by the Ford and Rockefeller Foundations with the help and approval of the Government of the Philippines. Today IRRI is one of 18 nonprofit international research centers supported by the Consultative Group on International Agricultural Research (CGIAR). The CGIAR is sponsored by thc Food and Agriculture Organization of the United Nations (FAO), the International Bank for Reconstruction and Development (World Bank), and the United Nations Development Programme (UNDP). Its membership comprises donor countries, international and regional organizations, and private foundations. IRRI receives support, through thc CGIAR, from a number of donors including FAO, UNDP, World Bank, European Economic Community, Asian Development Bank, Rockefeller Foundation, Ford Foundation, and thc international aid agencies of the following governments: Australia, Belgium, Canada, People’s Republic of China, Denmark, Finland, France, Germany, India, Islamic Republic of Iran, Italy, Japan, Republic of Korea, The Netherlands, Norway, Philippines, Spain, Sweden, Switzerland, United Kingdom, and United States. The responsibility for this publication rests with the International Rice Research Institute. Copyright © International Rice Research Institute 1994. All rights reserved. Except for quotations of short passages for the purpose of criticism and review, nn part of this publication may be reproduced, stored in retrieval systems, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior permission of IRRI. This permission will not be unreasonably withheld for use for noncommercial purposes. IRRI does not require payment for the noncommercial use of its published works, and hopes that this copyright declaration will not diminish the bona fide use of its research findings in agricultural research and development. The designations employed in the presentation of the material in this publication do not imply the cxpression of any opinion whatsoever on the part of IRRI concerning the legal status of any country, territory, city, or area, or of its authorities, or the delimitation of its frontiers or boundaries. International Rice Research Institute P.O. Box 933, 1099 Manila, Philippines FAX: (63-2) 818-2087, 522-4240 Electronic mail: IN%"Postmaster@IRRI.CGNET.COM" Telex: (ITT) 40890 RICE PM (CWI) 14519 IRILB PS (RCA) 22456 IRI PH (CWI) 14861 IRI PS ISBN 971-22-0049-3
Page 4 and 5: Contents Foreword v Preface vi Part
Page 6 and 7: Foreword Intensive collaboration ch
Page 8: Part 1 Introduction CHAPTER 1 Rice
Page 11 and 12: tional levels (Kahn 1988). Quaranti
Page 13 and 14: Morphology of the mature plant Matu
Page 15 and 16: two-celled, besifixed, deeply sagit
Page 18 and 19: Part 2 Procedures for seed health e
Page 20 and 21: CHAPTER 3 Equipment J.K. Misra, T.W
Page 22: 3.2 Dry seed inspection using a mag
Page 25 and 26: Example. In a two-stage random samp
Page 27 and 28: solid pointed end. The tube and sle
Page 30: CHAPTER 5 Dry seed inspection J.K.
Page 33 and 34: Detection Blotter and agar plate me
Page 35 and 36: Results: Examine plates for charac-
Page 37 and 38: Extraction and isolation It is esse
Page 39 and 40: GRINDING METHOD (FOR SMALL-SCALE SE
Page 41 and 42: flagellum. Cells occur singly, in p
Page 43 and 44: 7.6 Oxidase test reaction. L = colo
Page 45 and 46: 7.9 Production of 2-ketogluconate.
Page 47 and 48: 1. Grow pathogen-free plants under
Page 49 and 50: 7.12 a. P. glumae type A colony on
Page 51 and 52: A1. Macerate 100 seeds in 10 ml of
Page 53 and 54:
Results: 7.Streak the bacteria onto
Page 55 and 56:
Sieve method (Fig. 8.1) Procedure:
Page 58 and 59:
CHAPTER 10 Field inspection J.K. Mi
Page 60 and 61:
Table 10.2. continued Disease Fungi
Page 62 and 63:
Table 10.2. continued Disease Growt
Page 64:
additional precaution, the multipli
Page 67 and 68:
terracoat (5-ethoxy-3- trichloromet
Page 70:
Part 3 Pests and pathogens CHAPTER
Page 73 and 74:
elevant tests for determining the i
Page 75 and 76:
Table 12.1 continued Family and spe
Page 77 and 78:
Cryptolestes pusillus Schonherr The
Page 79 and 80:
Rice weeviI Scientific name: Sitoph
Page 82 and 83:
CHAPTER 14 Fungal pathogens J.K. Mi
Page 84 and 85:
THE DISEASE—BROWN SPOT Sir John W
Page 86 and 87:
Symptoms Glumes are discolored, and
Page 88 and 89:
y low relative humidity. Released a
Page 90 and 91:
THE DISEASE—STEM ROT Stem rot was
Page 92 and 93:
THE DISEASE—SHEATH BLIGHT Sheath
Page 94 and 95:
Sarocladium oryzae is seedborne. Th
Page 96:
Although not currently considered a
Page 99 and 100:
Pseudomonas fuscovaginae Pathogen:
Page 101 and 102:
See Chapter 7 and Figure 15.4a for
Page 103 and 104:
Infected seeds and contaminated wat
Page 105 and 106:
For further details, see the Common
Page 107 and 108:
nonfluorescent) and Erwinia herbico
Page 109 and 110:
Franklin M T, Siddiqi M R (1972) Ap
Page 111 and 112:
Tai F L, Siang W N (1948) 'I-chu-hs
Page 113 and 114:
106 Rice seed health testing manual
Page 115 and 116:
108 Rice seed health testing manual
Page 117 and 118:
After autoclaving the medium, add t
Page 119 and 120:
smoniloid = having swellings at reg
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