IonWorks Quattro Assay - Molecular Devices

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vinpocetine verapamil Tetrodotoxin tetracaine Standard 2 standard 1 100 80 60 40 20 hNav1.7 Peak Current 0 IC 50 2.9 µM at -140 mV IC 50 1.1 µM at -70 mV 120 0.01 0.1 1 10 100 µM 100 80 60 40 20 IC 50 25.7 µM at -140 mV 0 IC 50 11 µM at -70 mV 0.01 0.1 1 10 100 µM hNav1.7 Peak Current 120 100 80 60 40 20 0 140 120 100 80 60 40 20 State-dependent block of standard Nav blockers on Quattro lamotrigine dibucaine 10000 -140 mV -70 mV IC 50 >100 µM at -140 mV IC 50 87.6 µM at -70 mV 100 0.1 1 10 100 1000 µM propafenone verapamil 1 (+/-)-fluoxetine (+/-)-reboxetine (R,R)-reboxetine (S)-(-)bupivacaine ambroxol amiodarone cinnarizine clomipramine dibucaine haloperidol imipramine lamotrigine mexiletine propafenone R-(-)-fluoxetine IC 50 9.9 µM at -140 mV IC 50 8.1 µM at -70 mV 0.01 0.1 1 10 100 µM 0.01 1E-4 hNav1.7 Peak Current hNav1.7 Peak Current IC50 (µM) riluzole S-(+)-fluoxetine 0
Nav1.8 currents on IonWorks IonWorks HT IonWorks Quattro (PPC) D7 Patch Plate 0.5 Scan #2 0.4 0.3 0.2 0.1 Current (nA) 0.0 -0.1 -0.2 -0.3 -0.4 -0.5 0 5 10 15 20 25 Time (msec) I Na >100 pA Total Seals Number of Cells 28 290 Expression Rate 10 CsCl 140 mM and TTX 300 nM
Page 1 and 2: IonWorks Assays for Nav Channels Bi
Page 3 and 4: Sodium Channel Complexes n n α1 (>
Page 5 and 6: Nav1.7 Loss of Function Causes Inse
Page 7 and 8: FLIPR-384: ΔVm Can Identify Blocke
Page 9 and 10: Blocker Characterization with IonWo
Page 11 and 12: Correlation Between Assays IonWorks
Page 13 and 14: Nav1.5 Block by Propafenone: 80 60
Page 15 and 16: Need for IonWorks Quattro Assay n G
Page 17 and 18: I-V curves of Nav1.7 and Nav1.5 on
Page 19: Screening Nav1.7 State-Dependent Bl
Page 23: Acknowledgements Neuroscience Howar

IonWorks Quattro Assay - Molecular Devices

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