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XENOSEP®
EPA
1664A Std.
Plus 14 g
Solids
SPE Systems for
EPA Method 1664A
The Easiest, Most
Cost Effective Way to
Increase Productivity
“With Xenosep SPE
Filters, we experienced a
significant reduction in
labor and solvent usage
when compared to our old
brand of SPE disks.”
J. D. Waggoner, Managing Partner,
Inovatia Laboratories
93.4% HEM
Recovery
Filtration
Complete in Less
than 1 Minute
Making the Difference in Separation Products

Step 1
XENOSEP ®
SPE Systems for
EPA Method 1664A
Step 3
Step 2
Extract Samples 5 Times
Faster, Distill Samples 3 Times
Faster, and Significantly
Lower Cost Per Test...
Step 1
Extract 1 L Sample
17 seconds is all it takes to filter a 40 mg/L* OPR and
achieve 98% recovery. That’s 50 times faster than a
typical single layer SPE filter with 89% recovery.
In addition, triple layer Xenosep SPE Filters have
5 times the filtration capacity already built-in for the
MS, MSD, and hard-to-filter samples.
*As required by EPA Method 1664A
Three Simple Steps
Increase Productivity,
Saves Time and Money...
Step 2
Elute and Dry Extract
Less filter clogging enables more efficient filter drying
and eluting with less solvent. Xenosep sodium sulfate
columns save time by eliminating off-line drying steps
or repouring the extracted water sample back into the
separatory funnel.
Step 3
Distill and Oven Dry Extract
Less extraction solvent means faster distillation.
Final hexane extracts distill to dryness in about 10
minutes. Unique flat sided Xenosep flasks reduce
oven drying times by 67% so you get QC tests
in the dessicator 3 times faster than the other
options below.
Compare the Options
Process Step Xenosep ® SPE Typical SPE Hexane LLE
Extract-Elute-Dry 8.8 min. 26.5 min. 46.0 min.
Distill-Oven Dry 21.0 min. 50.0 min. 60.0 min.
Time to Dessicator 29.8 min. 76.5 min. 106.0 min.
Estimated Labor/Batch of 20 4 hrs. 6 hrs. 8 hrs.
Xenosep Labor Savings/Batch 2 hrs. 4 hrs.

The First SPE Filter and Glass
Apparatus Specially Designed for
Dirty Wastewater Samples
Conditioning/Filtration Apparatus
• Short wide funnel for easy access
and effective glassware rinsing
• Built-In space for optional prefilters,
filter aids, and/or drying agent additions
to “wet” solids
• Secure coupler maximizes effective
filtration area and prevents assembly
misalignment
• Teflon ® encapsulated O-ring seal
eliminates solvent and vacuum leaks
without extractables
• Solid rimmed stainless steel support
prevents analyte bypass
• Flared collection tube for easy removal
• Wider internal diameter stem maximizes
filtration rates
• Universal Ts 40/35 joint connects to
elution apparatus or standard multi-place
manifold, optimized to accept 30 mm OD
vials or collection tubes
• Easy to clean glassware minimizes breakage
and fits standard washers

Integral Design Maximizes Filtration
Efficiency, Simplifies Analyte Recovery,
and Ensures Reproducible Results
Elution/Cleaning Apparatus
• Unique integral apparatus increases productivity
and lowers cost per result
• Disposable prepacked sodium sulfate cartridge
maximizes recoveries, speed, and eliminates
off-line transfers
• Dedicated station for sample elution,
drying and CIP (clean-in-place) glassware
solvent rinsing
• Universal TS 24/25 joint for collection into
standard TS 24/40 flask
• Flat sided flask gently evaporates extract
to increase recoveries and lower MDLs
Distillation/Evaporation Apparatus
• Compact cross-over condenser design simplifies
solvent recovery and provides analyst protection
• Flat sided flask optimized to reduce
distillation/evaporation times
• N 2 inlet provided for safety and faster
distillation/evaporation

Xenosep ® SPE Fast Throughput
High Capacity Filters
Unique Triple Layer Construction Offers Fastest
Sample Throughput and Highest Filtration Capacity
• No emulsions or Freon ® non-compliance
• Assure legally defensible data
• Achieve higher quality results
• Reliable and consistent performance
• Decrease sample turnaround time
• Eliminate additional prefilter expense
• Significantly reduce operating cost
Three Filter Layers Trap Different Size
Solids to Maximize Speed and
Filtration Capacity
Filtration Extraction Elution
5-25 µm Prefilter
1-5 µm Prefilter
Adsorbent Layer
Filter Dirty Samples 10 Times Faster
Sample Filtered, (mL)
1200
1000
800
600
400
200
Xenosep ® SPE Filters 1 L
in 38 Sec. (Recovery 93.4%)
Sample:
40 mg/L* EPA 1664A Std.
Plus 14 g of Solids Added
Typical SPE Filters 1 L in
6.5 Min. (Recovery 79.9%)
Cutaway views of how the triple layer Xenosep SPE Filter
separates analytes from particulate and unwanted
soluble interferences
Green–3 µm Particulates
Red–Oil and Grease Analytes
Black–8 µm Particulates
Blue–Soluble Interference
Brown–30 µm Particulates
• •
0
0 1 2 3 4 5 6 7 8 9 10
Time (Minutes)
Temperature: 21°C, Vacuum: 15˝ Hg
SPE Filters–47 mm Diameter
Extra Thick Fluffy Layer Suspends
Solids Filter Cake and Prevents
Premature Clogging of Dirty Samples
Why are 40 mg/L PAR Comparisons Important
EPA Method 1664A requires the PAR concentration as
follows: “The weight must be 40 ± 1 mg” (Section
7.10.3 NOTE) and at least this amount are required for
the QCS, OPR, MS, MSD, and IPR samples. (Sections
9.8, 9.7, 9.6.1, 9.3.1, 9.2.2, 7.11 and 7.10) Although
certain method modifications are allowed, a change in
the PAR concentration is not expressly permitted and
“shall be considered a major modification subject to
application and approval of alternate test procedures
under CFR 136.4 and 136.5.” (Sections 1.7 and 1.8)
Why expose the defensibility of your data to
unnecessary legal risk Use 40 mg/L QC samples and
achieve higher quality results with Xenosep SPE Filters.
Request your free samples today online at
www.xenosep.com/contact.html.
PAR (Precision and Recovery); QCS (Quality Control Sample); OPR (Ongoing
Precision and Recovery); IPR (Initial Precision and Recovery); MS (Matrix Spike);
MSD (Matrix Spike Duplicate)
PAR Standard Filtered (mg)
240
200
160
120
80
40
SPE Speed and Filtration Capacity
40 mg/L* EPA 1664A PAR Standard
Xenosep SPE Filters 1 L in
17 Sec. (Recovery 97.6%)
Typical SPE Filters 1 L in 19.2 minutes (Recovery 89.3%)
0 2 4 6 8 10 12 14 16 18 20
Time (Minutes)
Temperature: 21°C, Vacuum: 15˝ Hg
SPE Filters–47 mm Diameter
Xenosep SPE Filters 5.5 L
in 20 Min. (Recovery 95.5%)
Xenosep SPE Filters Have FIVE
Times Greater Filtration Capacity
*As required by EPA Method 1664A
6
5
4
3
2
1
0
PAR Standard Filtered (L)

Ordering Information for Xenosep ® SPE Systems
Xenosep SPE Systems for performing EPA Method 1664A
are available in convenient Starter Kits for laboratories just
starting out. Starter Kits include all components required to
process one sample at a time. Additional components are
available for scale up as required.
Xenosep Manifold Kits are available for a three position
manifold and include components necessary to process
three samples at a time. If your laboratory has existing
glassware or manifolds, please visit our web site at
www.xenosep.com or call 1.866.936.6737 for assistance.
Note: Lab stand and
clamps not included
Xenosep ® SPE Starter Kit
Part No. Description
26616 Starter Kit, 47 mm, 1 Place
Part No. for Kit Components Included*
Quantity
26603; 26604; 26610; 26611; 26613; 26614; 26615; 26508;
26514; 26515; 26516; 26517
1 each
26608 2 each
26512 3 each
26513 7 each
*See Replacement Parts for description
Additional Apparatus Assembly Kits
Part No. Description Quantity
26511 Distillation Kit, Complete
26513; 26514; 26515 1 each
26512 3 each
26526 Flask Kit, Flat Sided, TS 24/25, 125 mL
26513 12 each
26600 SPE Filtration Assembly, 47 mm, Starter
26603; 26604; 26610; 26611; 26613 1 each
26608 2 each
26601 SPE Filtration Assembly, 47 mm, Manifold
26603; 26605; 26610; 26611; 26613 1 each
26608 2 each
26620 Consumables Kit, 24 Tests
24547; 26520; 26524 24 each
Consumables
Part No. Description Quantity
24547 SPE Filters, 1664A, 47 mm 48
26520 Pipets, Transfer, Polyethylene, 15 mL 48
26524 Column, Inline, Sodium Sulfate, 10 g 48
26528 Fibers, Prefilter, 50 g 1
26532 EPA 1664A Std., 1 x 100 mL, 4 mg/mL 1
26533 EPA 1664A Std., 12 x 10 mL, 4 mg/mL 12
©Copyright, Xenosep Technologies, 2006 Printed in USA
Xenosep ® is a registered trademark of Xenosep Technologies LLC
Freon ® , Teflon ® , and Viton ® are registered trademarks of DuPont
Tygon ® is a registered trademark of Saint-Gobain Performance Plastics
Note: Manifold sold separately
Xenosep ® SPE Manifold Kit
Part No. Description
26618 Manifold Kit, 47 mm, 3 Place
Part No. for Kit Components Included*
Quantity
26615; 26512; 26516; 26519 1 each
26603; 26605; 26610; 26611; 26507; 26613 3 each
26608 6 each
*See Replacement Parts for description
Replacement Parts and Components
Part No. Description Quantity
26507 Vials, Collection, 30 x 125 mm 1
26508 Tube, Collection, 30 x 200 mm 1
26512 Clamp, Polyoxymethylene Acetal, TS 24/25 1
26513 Flask, Flat Sided Erlenmeyer, TS 24/25, 125 mL 1
26514 Flask, Round Flat Bottom, TS 24/25, 125 mL 1
26515 Condenser, Crossover w/#2HB 1
26516 Eluter, Glass, w/#2HB 1
26517 Stopcock, Glass, 120° 3 way, 4 mm ID 1
26519 Stopcock Assembly, Manifold 1
26523 Vent Assembly, Manifold 12
26603 Funnel, 300 mL, 47 mm 1
26604 Holder, Starter, 47 mm 1
26605 Holder, Manifold, 47 mm 1
26608 O-Rings, FEP-Viton ® , Coupler, 47 mm 1
26610 Coupler, PTFE, 47 mm 1
26611 Support, Stainless Steel, 47 mm 1
26613 Clamp, Aluminum, 47 mm 1
26614 Flask, TS 40/35, 1 L 1
26615 Tubing, Tygon ® , 3/8˝ ID x 5/8˝ OD, 3´ 1
26619 Manifold, Complete, Stainless Steel, 3 Place 1
Making the Difference in
Separation Products
Xenosep Technologies LLC
508 Main Street, Boonton, NJ 07005
866.XENOSEP (866.936.6737) • 973.239.0247 • FAX: 973.239.4133
E-mail: info@xenosep.com • www.xenosep.com