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Landscapes Forest and Global Change - ESA - Escola Superior ...

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G.M. Pastur et al. 2010. Indirect estimation of l<strong>and</strong>scape uses by Lama guanicoe <strong>and</strong> domestic herbivorous<br />

154<br />

2. Methodology<br />

The study was conducted in 100 km² of Tierra del Fuego Isl<strong>and</strong> (Argentina) (54°20' SL, 67°52'<br />

WL), where open l<strong>and</strong>s covered 40.0% of the area (grass-l<strong>and</strong>s 36.5% <strong>and</strong> peat-l<strong>and</strong>s 3.5%),<br />

forests covered 59.8% (Nothofagus antarctica forests 19.3% <strong>and</strong> N. pumilio forests 49.5%,<br />

classified as primary unmanaged 26.8%, recently harvested 9.1% <strong>and</strong> old harvesting 13.6%),<br />

<strong>and</strong> lagoons <strong>and</strong> lakes covered 0.2% (Fig. 1). Recently harvested forests (1-5 years) were cutted<br />

using a variable retention method (Martínez Pastur et al. 2009), which include non-harvested<br />

aggregated retention areas (30% of the st<strong>and</strong>) <strong>and</strong> harvested areas with dispersed retention (70%<br />

of the st<strong>and</strong>). Old harvesting (>5 years) was done through selective cuts. Climate is<br />

characterized by short, cool summers <strong>and</strong> long, snowy <strong>and</strong> frozen winters. Mean monthly<br />

temperatures vary from about -7ºC to 14ºC. Absolute temperatures range from -17ºC in July to<br />

22ºC in January. The growing season extends for about 5 months, <strong>and</strong> only 3 months per year<br />

are frost-free. Precipitation is near 400 mm per year, <strong>and</strong> average wind speed is 8 km.h -1 ,<br />

reaching up to 100 km.h -1 during storms (Lencinas et al. 2008). A total of 205 floristic surveys<br />

were obtained recording the cover percentage of vegetation in different site types. Vascular<br />

plants (Dicotyledonae, Monocotyledonae <strong>and</strong> Pteridophytae) were taxonomically classified by<br />

species <strong>and</strong> determined their origin (native or exotic), following Moore (1983) <strong>and</strong> Correa<br />

(1969-1998). Beside this, each species was classified according their relative abundance in<br />

common (more than 0.02% covers in average for the entire census) or rare (less than 0.02%).<br />

Figure 1: Study area, where: yellow = open l<strong>and</strong>s, brown = Nothofagus antarctica forests, green = N.<br />

pumilio forests (dark green = primary unmanaged, green = recently harvested, pale green = old<br />

harvesting), <strong>and</strong> blue = lagoons <strong>and</strong> lakes.<br />

Feces of native (Lama guanicoe) <strong>and</strong> domestic (Bos taurus <strong>and</strong> Ovis aries) herbivorous<br />

were collected along the four seasons (spring, summer, autumn <strong>and</strong> winter). In each sampling,<br />

four areas including different site types were selected, <strong>and</strong> five feces were collected <strong>and</strong> mixed<br />

to complete one pool sample (n = 4 per season per herbivorous species). Pool samples were<br />

oven-dried at 60ºC for 48 h, grounded to

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