Millex®-GV Sterilizing Filter Unit - Biocenter

User Guide
Millex ® -GV
Sterilizing Filter Unit
(For Low-Binding Sterilization)
■ Single use only
■ Sterile
■ Non-pyrogenic
■ Do not use this product as an in-line filter for intravenous administration
HCM0 518 01 (1000/box)

Notice
The information in this document is subject to change without notice and should not be
construed as a commitment by Millipore Corporation. Millipore Corporation assumes no
responsibility for any errors that may appear in this document. This manual is believed to be
complete and accurate at the time of publication. In no event shall Millipore Corporation be
liable for incidental or consequential damages in connection with or arising from the use of
this manual.
Copyright 2005. Millipore Corporation. Printed in Ireland. All rights reserved.
Millipore, Durapore and Millex are registered trademarks of Millipore Corporation.
Luer Lok is a trademark of Becton-Dickinson and Company.
Freon is a trademark of E.I. du Pont de Nemours & Co., Inc.
P36381, Rev. A, 10/05
0459
This product is manufactured by:
Millipore Ireland BV
Carrigtwohill
Co. Cork

Introduction
This document provides compatibility information, operating steps, and specifications
for the Millex-GV sterilizing filter. This bi-directionally supported, low-binding sterilizing
unit is designed to filter aqueous, proteinaceous substances where minimal sample loss
is desired. It removes microorganisms, particles, precipitates, and undissolved powders
larger than 0.22 micron (µm). This single-use product consists of a 0.22 µm Durapore ®
membrane filter sealed in a PVC housing. It is sterile, non-pyrogenic, and non-toxic.
Typical applications include the sterile filtration of tissue culture media, media additives,
protein solutions,virus suspensions, monoclonal antibodies, DNA solutions, antibiotics,
and lymphokines.

2 www.millipore.com/millex
Chemical Compatibility
The Millex-GV filter is compatible with most aqueous solutions. You may use it to filter
the agents listed in the following chart. This information was developed from technical
publications, materials suppliers, laboratory tests, and field evaluations, etc., and is believed
to be reliable. However, because of variability in temperature, concentrations,
duration of exposure, and other factors outside of our control that may affect the use of
the unit, Millipore does not provide or imply a warranty with respect to such information.
You should test the agents that are not listed with the Millex-GV filter prior to use.
Chemical
Acetic acid (glacial)
Acetic acid (5%)
Aliphatic ethers
Ammonium hydroxide (6N)
Amyl acetate
Amyl alcohol
Benzyl alcohol (1%)
Boric acid
Brine (sea water)
Butyl alcohol
Carbon tetrachloride
Ethyl alcohol
Ethylene glycol
Formaldehyde
Freon ® solvent, TF or PCA
Glycerine (glycerol)
Helium
Hexane
Hydroflouric acid
Hydrogen (gas)
Hydrogen peroxide (3%)
Hypo (photo)
Isobutyl alcohol
Isopropyl acetate
Methyl alcohol
Mineral spirits
Nitrogen
Paraldehyde
Pentane
Pet base oils
Petroleum ether
Phenol (0.5%)
Pyridine

Millex-GV Sterilizing Filter 3
How to Use the Millex-GV Filter Unit
This section lists warnings and cautions and provides steps to use the Millex-GV filter unit.
WARNINGS:
■ To ensure sterility, do not use this product if the package is damaged.
■ Do not use this product as an in-line filter for intravenous administration; it
was not designed for that purpose.
■ Do not use with syringes smaller than 10 cc because pressures in excess of
the maximum pressure rating may be reached, potentially causing damage to
the filter unit and/or personal injury.
CAUTIONS:
■ Do not resterilize or reuse the Millex-GV filter unit.
■ Do not use the Millex-GV unit at temperatures above 45 °C (113 °F).
■ Do not use the same Millex-GV unit to filter solutions in both directions.
■ Do not use the Millex-GV unit to filter emulsions or suspensions.
■ Do not use the Millex-GV unit to filter 5 milligrams (mg) or less of active drug materials
unless you have performed binding studies.

4 www.millipore.com/millex
Directions for the Millex-GV Sterilizing Filter Unit
1
2 3
Fill the syringe with the
solution you want to filter.
4
Aseptically remove the
cover from the package
that contains the
Millex-GV filter.
5
Attach the syringe to the
Millex-GV filter and remove
the assembly from the package.
(Attach needle to Luer
Slip outlet if necessary.)
Hold the syringe with the filter (and
needle if attached) pointing up and top
it off by pushing a few drops through it.
(Make sure you do not contaminate the
underside of the Millex-GV filter with
your fingers.)
Insert the needle (if attached) and push
the plunger to deliver the filtered solution.

Millex-GV Sterilizing Filter 5
Specifications
Materials
Dimensions
Temperature limit
Pressure limit at 25 °C
Filtration volume
Hold-up volume
Connections
Sterilization method
Flow rate at 25 °C
Hydrophilic Durapore (PVDF) membrane, type GV;
Polyvinyl Chloride (PVC) housing
inlet to outlet 25 millimeters (mm) (1.0 in.)
diameter 29 mm (1.13 in.)
filtration area 4 centimeters 2 (cm 2 ) (0.62 in 2 )
pore size 0.22 µm
45 °C (113 °F) maximum
75 psig (5 bar) inlet and differential maximum
1.0 milliliter (mL) to 100 mL
≤ 0.1 mL after air purge
Female, Luer Lok ® inlet; male, Luer slip outlet
Ethylene Oxide Gas
Typical initial average flow rate for water at 30 psig: 40 mL/min

Technical Assistance
For more information, contact the Millipore office nearest you. In the U.S., call 1-800-
MILLIPORE (1-800-645-5476). Outside the U.S., see your Millipore catalogue for the
phone number of the office nearest you or go to our web site at www.millipore.com/
offices for up-to-date worldwide contact information. You can also visit the tech service
page on our web site at www.millipore.com/techservice.
Warranty
This product is covered by Millipore’s standard limited warranty, as set forth in
Millipore’s Bioscience Catalogue.