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Highlight a place to call home - BorneoTalk Official Website

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Education<br />

(left <strong>to</strong> right) Assoc. Prof Dr<br />

M.A Affan, Assoc. Prof Dr<br />

Zainab Ngaini and Senior<br />

Lecturer Dr Ho Wei Seng<br />

At UNIMAS (University Malaysia<br />

Sarawak) they have successfully<br />

developed a kit, which can do exactly<br />

what the industries are looking for in<br />

the field of amplifying DNA via PCR<br />

without prior DNA purification. Aptly<br />

<strong>call</strong>ed a fasTiP-X Kit, the machine<br />

simplifies the process while also<br />

saves cost and time. It also does not<br />

use liquid nitrogen nor hazardous<br />

chemicals and therefore suitable for<br />

high-throughput of genome analyses.<br />

DNA<br />

extraction<br />

of plant samples<br />

Researcher: Dr Ho Wei Seng<br />

It is a fact that majority of people<br />

in this world do not know<br />

anything about DNA extraction of<br />

plant samples nor the process of<br />

identifying compounds and other<br />

plant content in the samples. To<br />

those in the research industry like<br />

forestry, biological and herbal<br />

labora<strong>to</strong>ries, fac<strong>to</strong>ries and chemistry,<br />

such information is vital for their<br />

work. As such, the faster these<br />

simple extractions can be done and<br />

results produced, the better it will<br />

be for them <strong>to</strong> move in<strong>to</strong> the next<br />

step or stage of research on the<br />

materials.<br />

With the new device, there are only<br />

three simple steps. Firstly, transfer the<br />

plant samples in<strong>to</strong> the fasTip-X Extract<br />

Buffer by <strong>to</strong>uching the samples with a<br />

Harris Uni-core puncher. Next, incubate<br />

<strong>to</strong> lyses the plant cells and finally use<br />

the incubated solution for polymerase<br />

Chain Reaction (PCR) amplification.<br />

As can be seen the whole process<br />

is simple and easy for anyone with<br />

competent <strong>to</strong> handle. The time fac<strong>to</strong>r<br />

is important in the processing as results<br />

can be gathered in a short time and<br />

also very minimum sample material is<br />

required. The sample can be amplified<br />

>3 kb and works with many varieties<br />

of plant samples. It is also obvious that<br />

the process is safe as no hazardous<br />

chemicals are used. Most importantly,<br />

the device is cost effective and the<br />

difference could be as much as US$7<br />

when compared <strong>to</strong> conventional<br />

extraction methods per sample.<br />

For better and closer look in<strong>to</strong><br />

these new findings at UNIMAS,<br />

prospective inves<strong>to</strong>rs and businesses<br />

should directly consult the university<br />

and work in collaboration with<br />

them. After all, what is good for<br />

the research team will eventually<br />

be useful and cost effective for the<br />

industries in the long run.<br />

Faculty of Resource Science<br />

and Technology<br />

Universiti Malaysia Sarawak<br />

94300 Kota Samarahan, Sarawak, Malaysia.<br />

Tel: +6082 583161 Fax: +6082 583160<br />

www.unimas.my<br />

Apr-Jun2012<strong>BorneoTalk</strong> | 65

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