Preface - Ous-research.no

Experimental microsurgery and Transplantation
Figure 1
Figure 6. Cell viability of hepatoma cell lines and primary hepatocytes
with Sorafenib and EGF for 2 days.Primary hepatocytes
showed higher cell viability compared with hepatoma cell lines
after being cultured for 48 h Sorafenib (0-12.5μM) induction and
EGF (10 μM) stimulation.
Results:
After Sorafenib exposure, primary hepatocytes showed
higher cell viability, proliferation and stronger Raf/MAPK
kinase activity compared with all hepatoma cell lines. In the
in vivo model, the tumor volumes and number of intrahepatic
and distant metastases were significantly decreased
(P0.05).
The current project indicates that Raf targeted inhibitor
Sorafenib can reduce tumor proliferation and metastasis
without retarding liver regeneration. This is a result of the
different responses of primary hepatocyte and hepatoma
cells to Raf/MAPK inhibition.
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