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treated and algal extract treated phagocytes. Thus, the polysaccharidic fraction from P. cruentum did not show ability to increase the respiratory burst activity of sole phagocytes, neither alone or in the presence of P. damselae subsp. piscicida, being stimulation index obtained 1.042 ± 0.069. Superoxide anion production of phagocytes isolated from sole inoculated with P. cruentum polysaccharide and P. damselae subsp. piscicida bacterin are shown in Figure 2. Inoculation of the polysaccharide or the bacterin alone resulted in a significant decrease (P
Intraperitoneal inoculation of the polysaccharidic fraction of P. cruentum to sole specimens did not induce an increase of the respiratory burst activity. On the contrary, the respiratory burst activity decreased in fish inoculated with polysaccharide fraction or with bacterin within 24 h post-inoculation. This decrease in respiratory burst activity may be due to a immunosupression caused by stress after handling [39, 40] as it is not observed after 7 days of the inoculation. In short, the results obtained in vivo indicate that the polysaccharidic fraction from P. cruentum does not enhance the respiratory burst activity compared to non inoculated fish. Acknowledgements Financial support was provided by the Ministry of Education and Science Spain (AGL2002-01488 and AGL 2005-02655) and Junta de Andalucía (RNM-295). The authors are grateful to Aula del Mar of Málaga (Spain) and PROMAN (Motril, Granada, Spain) for their help and participation in this study. P. Díaz-Rosales thanks Ministerio Español de Educación y Ciencia for a F.P.U. scholarship. 5. References [1] Dinis, MT, Ribeiro, L, Soares, F and Sarasquete, C. A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture 1999; 176: 27-38. [2] Zorrilla, I, Balebona, MC, Moriñigo, MA, Sarasquete, C and Borrego, JJ. Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damselae subsp. piscicida, from sole, Solea senegalensis (Kaup). Journal of Fish Diseases 1999; 22: 167-72. [3] Magariños, B, Romalde, JL, López-Romalde, S, Moriñigo, MA and Toranzo, AE. Pathobiological characterisation of Photobacterium damselae subsp. piscicida isolated from cultured sole (Solea senegalensis). Bulletin of European Association of Fish Pathologists 2003; 23: 183-9. 9
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FACULTAD DE CIENCIAS DEPARTAMENTO D
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Los ensayos que constituyen esta Te
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- Díaz-Rosales, P, Arijo, S, Chabr
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Sabe esperar, aguarda que la marea
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ÍNDICE / INDEX Papel de las activi
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ÍNDICE / INDEX 2. Photobacterium d
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RESUMEN Photobacterium damselae sub
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INTRODUCCIÓN 1. LA ACUICULTURA. EL
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INTRODUCCIÓN saxatilis) (Hawke et
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INTRODUCCIÓN 2.2. SINTOMATOLOGÍA
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INTRODUCCIÓN las epidemias surgida
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INTRODUCCIÓN et al., 1997). En P.
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INTRODUCCIÓN 3.1. ESTALLIDO RESPIR
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INTRODUCCIÓN radicales generados e
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INTRODUCCIÓN Tabla 2 Ejemplos de m
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INTRODUCCIÓN de parasitismo en el
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INTRODUCCIÓN razón de la búsqued
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INTRODUCCIÓN araquidónico, están
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INTRODUCCIÓN simplificar la admini
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INTRODUCCIÓN sino también cuando
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INTRODUCCIÓN La necesidad de mejor
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OBJETIVOS El trabajo planteado en e
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MATERIA L Y MÉTODOS La metodologí
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RESULTADO S Y DISCUSIÓN El primer
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RESULTADO S Y DISCUSIÓN 1996; Lync
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RESULTADO S Y DISCUSIÓN Una vez de
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RESULTADO S Y DISCUSIÓN inmunoesti
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RESULTADO S Y DISCUSIÓN cruentum s
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RESULTADO S Y DISCUSIÓN permiten c
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RESULTADO S Y DISCUSIÓN harían fa
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CONCLUSIONES Tras los estudios real
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ABSTRACT Photobacterium damselae su
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INTRODUCTION 1. AQUACULTURE. THE CU
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INTRODUCTION With regard to Solea s
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INTRODUCTION 3.1. RESPIRATORY BURST
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INTRODUCTION Some catalases have al
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INTRODUCTION pathogens, study of th
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INTRODUCTION increase their bacteri
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INTRODUCTION 4.3.1. Porphyridium cr
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INTRODUCTION Different mechanisms h
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A I M S
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M A T E R I A L S A N D M E T H O D
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R E S U L T S A N D D I S C U S S I
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RESULTS AND DISCUSSION resist 100 m
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RESULTS AND DISCUSSION present work
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RESULTS AND DISCUSSION reason, once
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RESULTS AND DISCUSSION great number
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RESULTS AND DISCUSSION dominant spe
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CONCLUSIONS Studies carried out on
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R R E F E R E N C I A S E F E R E N
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REFERENCIAS / REFEREN CES Aoki, T,
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REFERENCIAS / REFEREN CES Bell, MV,
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REFERENCIAS / REFEREN CES Dalmo, RA
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REFERENCIAS / REFEREN CES Hamaguchi
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REFERENCIAS / REFEREN CES Kono, Y &
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REFERENCIAS / REFEREN CES Magariño
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REFERENCIAS / REFEREN CES Ortuño,
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REFERENCIAS / REFEREN CES Salinas,
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REFERENCIAS / REFEREN CES Thompson,
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S E C C I Ó N D E A R T Í C U L O
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A RTÍCULO 1.1. A RTICLE 1.1.
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Journal of Fish Diseases 2003, 26,
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Page 169 and 170: Journal of Fish Diseases 2006, 29,
Page 177: A RTÍCULO 2.1. A RTICLE 2.1.
Page 180 and 181: Abstract The stimulatory effect of
Page 182 and 183: alga Porphyridium cruentum. For thi
Page 184 and 185: Feeding assays were carried out wit
Page 186 and 187: (10 8 bacterias ml -1 ) and used to
Page 188 and 189: are in agreement with the data repo
Page 190 and 191: unvaccinated fish. However, the ser
Page 192 and 193: immune response of gilthead seabrea
Page 194 and 195: [27] Duncan, PL and Klesius, PH. Ef
Page 196 and 197: (Macrogard) and alginic acid (Ergos
Page 198 and 199: [66] Salinas, I, Díaz-Rosales, P,
Page 201: Figure 1a 3 2.5 Stimulation index 2
Page 205: Figure 1c Stimulation index 3 2.5 2
Page 209: Figure 2b Stimulation index 3 2.5 2
Page 213 and 214: Effect of the extracellular polysac
Page 215 and 216: 1. Introduction Solea senegalensis
Page 217 and 218: On the other hand, β-1,3-glucan fr
Page 219: eaction was tested by adding supero
Page 223 and 224: defense mechanisms and protective i
Page 225: phagocytes from sole (Solea senegal
Page 229: Figure 1a Stimulation index 3 2.5 2
Page 233: Figure 2 Stimulation index 1.4 1.2
Page 237 and 238: Effect of dietary administration of
Page 239 and 240: 1. Introduction Senegalese sole cul
Page 241 and 242: 2.2. Fish and experimental design S
Page 243 and 244: 2.5. Challenge with Photobacterium
Page 245 and 246: the densitometric curves of the pro
Page 247 and 248: most efficient probiotics for aquac
Page 249 and 250: acterial diversity only the dominan
Page 251 and 252: [9] Verschuere, L, Rombaut, G, Sorg
Page 253 and 254: [28] Burr, G, Gathin, D and Ricke,
Page 255 and 256: WB, editors. Techniques in Fish Imm
Page 257: and host-specific communities of ac
Page 261: Table 1 Primer Sequence (5’-3’)
Page 265: Figure 1b Stimulation index 2.5 2 1
Page 269: Table 2 Primer Control Pdp11 Pdp13
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Figure 3 Pearson correlation [0.0%-
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que perdieras parte de tu tiempo en
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