Papel de las actividades superóxido dismutasa y catalasa en la ...

More documents

Recommendations

Info

Journal of Fish Diseases 2006, 29, 355–364 Superoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida P Díaz-Rosales, M Chabrillón, S Arijo, E Martinez-Manzanares, M A MoriÇigo and M C Balebona Department of Microbiology, Faculty of Sciences, University of Málaga, Malaga, Spain Abstract The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced by hydrogen peroxide or methyl viologen, or under iron depleted conditions. However, iron content of the growth medium influenced the levels of SOD and catalase activity in cells, these levels decreasing with iron availability of the medium. Comparison of virulent and non-virulent strains of P. damselae ssp. piscicida showed similar contents of SOD, but higher levels of catalase were detected in cells of the virulent strain. Incubation of bacteria with sole, Solea senegalensis (Kaup), phagocytes has shown that survival rates range from 19% to 62%, these rates being higher for the virulent strain. The increased levels of catalase activity detected in the virulent strain indicates a possible role for this enzyme in bacterial survival. Keywords: catalase, phagocyte, Photobacterium damselae subsp, Solea senegalensis, superoxide dismutase, virulence. Introduction Photobacterium damselae ssp. piscicida is a pathogen responsible for important losses in fish Correspondence Prof. M C Balebona, Department of Microbiology, Faculty of Sciences, University of Malaga, 29071 Malaga, Spain (e-mail: balebona@uma.es) aquaculture worldwide. The importance of extracellular products, the presence of iron uptake mechanisms and the capsular material as virulence factors in P. damselae ssp. piscicida are well documented (Magariños, Romalde, Bandín, Fouz & Toranzo 1992; Magariños, Pazos, Santos, Romalde & Toranzo 1994; Magariños, Romalde, Lemos, Barja & Toranzo 1995; Arijo, Borrego, Zorrilla, Balebona & Moriñigo 1998). However, information concerning mechanisms involved in the invasion and survival inside the host is scarce and results regarding interaction of P. damselae ssp. piscicida with phagocytes have been contradictory. While some authors have reported the presence of intact bacteria inside fish cells, suggesting the ability of P. damselae to survive as an intracellular pathogen (Kubota, Kimura & Egusa 1970; Nelson, Kawahara, Kawai & Kusuda 1981; Kusuda & Salati 1993; Noya, Magariños & Lamas 1995a; Noya, Magariños, Toranzo & Lamas 1995b), others have observed that this pathogen is highly susceptible to oxidative radicals generated during the macrophage respiratory burst (Skarmeta, Bandín, Santos & Toranzo 1995; Arijo et al. 1998). The reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 ), are produced by phagocytes in response to microbial infection. ROS constitute an important component of the innate active defence response against invading microorganisms by fish phagocytic cells. Therefore, bacterial pathogens must overcome the toxic effects of ROS to establish infections. Microorganisms have evolved systems to protect themselves from these highly toxic radicals. One of these protective pathways involves the production of detoxifying enzymes such as Ó 2006 Blackwell Publishing Ltd 355
Page 1:
FACULTAD DE CIENCIAS DEPARTAMENTO D
Page 5:
Page 9 and 10:
Los ensayos que constituyen esta Te
Page 11:
- Díaz-Rosales, P, Arijo, S, Chabr
Page 15:
Sabe esperar, aguarda que la marea
Page 19 and 20:
ÍNDICE / INDEX Papel de las activi
Page 21:
ÍNDICE / INDEX 2. Photobacterium d
Page 25:
RESUMEN Photobacterium damselae sub
Page 29 and 30:
INTRODUCCIÓN 1. LA ACUICULTURA. EL
Page 31 and 32:
INTRODUCCIÓN saxatilis) (Hawke et
Page 33 and 34:
INTRODUCCIÓN 2.2. SINTOMATOLOGÍA
Page 35 and 36:
INTRODUCCIÓN las epidemias surgida
Page 37 and 38:
INTRODUCCIÓN et al., 1997). En P.
Page 39 and 40:
INTRODUCCIÓN 3.1. ESTALLIDO RESPIR
Page 41 and 42:
INTRODUCCIÓN radicales generados e
Page 43 and 44:
INTRODUCCIÓN Tabla 2 Ejemplos de m
Page 45 and 46:
INTRODUCCIÓN de parasitismo en el
Page 47 and 48:
INTRODUCCIÓN razón de la búsqued
Page 49 and 50:
INTRODUCCIÓN araquidónico, están
Page 51 and 52:
INTRODUCCIÓN simplificar la admini
Page 53 and 54:
INTRODUCCIÓN sino también cuando
Page 55:
INTRODUCCIÓN La necesidad de mejor
Page 59:
OBJETIVOS El trabajo planteado en e
Page 63:
MATERIA L Y MÉTODOS La metodologí
Page 67 and 68:
RESULTADO S Y DISCUSIÓN El primer
Page 69 and 70:
RESULTADO S Y DISCUSIÓN 1996; Lync
Page 71 and 72:
RESULTADO S Y DISCUSIÓN Una vez de
Page 73 and 74:
RESULTADO S Y DISCUSIÓN inmunoesti
Page 75 and 76:
RESULTADO S Y DISCUSIÓN cruentum s
Page 77 and 78:
RESULTADO S Y DISCUSIÓN permiten c
Page 79:
RESULTADO S Y DISCUSIÓN harían fa
Page 83 and 84:
CONCLUSIONES Tras los estudios real
Page 85:
Page 89:
ABSTRACT Photobacterium damselae su
Page 93 and 94:
INTRODUCTION 1. AQUACULTURE. THE CU
Page 95 and 96:
INTRODUCTION With regard to Solea s
Page 97 and 98:
INTRODUCTION 3.1. RESPIRATORY BURST
Page 99 and 100:
INTRODUCTION Some catalases have al
Page 101 and 102:
INTRODUCTION pathogens, study of th
Page 103 and 104:
INTRODUCTION increase their bacteri
Page 105 and 106:
INTRODUCTION 4.3.1. Porphyridium cr
Page 107 and 108:
INTRODUCTION Different mechanisms h
Page 109:
A I M S
Page 113:
M A T E R I A L S A N D M E T H O D
Page 117: R E S U L T S A N D D I S C U S S I
Page 120 and 121: RESULTS AND DISCUSSION resist 100 m
Page 122 and 123: RESULTS AND DISCUSSION present work
Page 124 and 125: RESULTS AND DISCUSSION reason, once
Page 126 and 127: RESULTS AND DISCUSSION great number
Page 128 and 129: RESULTS AND DISCUSSION dominant spe
Page 131 and 132: CONCLUSIONS Studies carried out on
Page 133: R R E F E R E N C I A S E F E R E N
Page 136 and 137: REFERENCIAS / REFEREN CES Aoki, T,
Page 138 and 139: REFERENCIAS / REFEREN CES Bell, MV,
Page 140 and 141: REFERENCIAS / REFEREN CES Dalmo, RA
Page 142 and 143: REFERENCIAS / REFEREN CES Hamaguchi
Page 144 and 145: REFERENCIAS / REFEREN CES Kono, Y &
Page 146 and 147: REFERENCIAS / REFEREN CES Magariño
Page 148 and 149: REFERENCIAS / REFEREN CES Ortuño,
Page 150 and 151: REFERENCIAS / REFEREN CES Salinas,
Page 152 and 153: REFERENCIAS / REFEREN CES Thompson,
Page 155: S E C C I Ó N D E A R T Í C U L O
Page 159: A RTÍCULO 1.1. A RTICLE 1.1.
Page 162 and 163: Journal of Fish Diseases 2003, 26,
Page 179 and 180: Effect of dietary administration of
Page 181 and 182: 1. Introduction Fish aquaculture is
Page 183 and 184: 2.2. Alga culture The red microalga
Page 185 and 186: spreading an aliquot of the bacteri
Page 187 and 188: The results obtained show that extr
Page 189 and 190: increase the resistance to diseases
Page 191 and 192: Ciencia for a F.P.U. scholarship. T
Page 193 and 194: [18] Bansemir, A, Blume, M, Schröd
Page 195 and 196: [38] Sealey, WM and Gatlin, DM III.
Page 197 and 198: antigens using a bacterin containin
Page 199: Figure legends Figure 1. Respirator
Page 203: Figure 1b 3 2.5 Stimulation index 2
Page 207: Figure 2a Stimulation index 3 2.5 2
Page 211: A RTÍCULO 2.2. A RTICLE 2.2.
Page 214 and 215: Abstract The potential effect of ex
Page 216 and 217:
The aim of this work is to evaluate
Page 218 and 219:
2.4. Isolation of head kidney phago
Page 220 and 221:
treated and algal extract treated p
Page 222 and 223:
[4] Arijo, S, Chabrillón, M, Díaz
Page 224 and 225:
[24] Castro, R, Piazzon, MC, Zarra,
Page 227:
Figure legends Figure 1. Respirator
Page 231:
Figure 1b Stimulation index 3 2.5 2
Page 235:
A RTÍCULO 2.3. A RTICLE 2.3.
Page 238 and 239:
Abstract The effects of the dietary
Page 240 and 241:
suggesting the ability of the bacte
Page 242 and 243:
separated at the interface were col
Page 244 and 245:
2.6.3. PCR amplification Two differ
Page 246 and 247:
3.3. Intestinal microbiota analysis
Page 248 and 249:
Several authors have reported a sti
Page 250 and 251:
Acknowledgements The authors thank
Page 252 and 253:
[19] Villamil, L, Figueras, A, Plan
Page 254 and 255:
[37] Chabrillón M, Rico RM, Arijo,
Page 256 and 257:
[55] Sakata, T. Microflora in the d
Page 259:
Figures legends Table 1. Primers an
Page 263:
Figure 1a 2.5 2 * Stimulation index
Page 267:
Figure 2 Cumulative mortality (%) 1
Page 271:
Table 3 Primer Bact-0968-GC-F & Bac
Page 275 and 276:
Agradecimientos / Acknowledgements
show all

Papel de las actividades superóxido dismutasa y catalasa en la ...

Create successful ePaper yourself

Delete template?

Save as template?