Papel de las actividades superóxido dismutasa y catalasa en la ...

Journal of Fish Diseases 2003, 26, 305–308
PDíaz-Rosales et al. Resistance of Photobacterium damselae to hydrogen peroxide
A significant (P < 0.05) increase in the survival
rates of the non-virulent strain was observed when
cultures were pulsed with hydrogen peroxide
compared with cells cultured until the stationary
phase. In contrast, this increase has not been
observed for the virulent strain, which always
showed higher survival regardless of the growth
phase or the pulse with hydrogen peroxide.
Peroxide induction of catalase and increased cell
survival have been reported for several bacterial
4 pathogens (Loewen, Switala & Triggs-Raine 1985;
Barnes et al. 1999b; Vattanaviboon & Mongkolsuk
2001). Results obtained in this study suggest that
peroxide-decomposing enzymes induced in the
strain Epoy only by peroxide treatment could
protect these cells from oxidation, whilst decreasing
survival rates observed in cells grown in other
conditions could be attributable to lower levels of
catalase and peroxidase activities. In contrast, the
high survival rates observed in the virulent strain in
stationary phase cultures, and in cells cultured in
the presence of iron or pulsed with hydrogen
peroxide suggest the presence of higher levels of
catalase activity in the cells grown under these
conditions, although a possible relationship with
virulence remains to be demonstrated. Furthermore,
the presence of a capsule in the virulent strain
may have an important role in the protection of
P. damselae subsp. piscicida cells against peroxide.
This capsule would partially contribute to
the increased survival of the virulent strain compared
with strain Epoy, a non-capsulated strain
(Magariños et al. 1996).
When bacteria were cultured under iron limited
conditions, a significant decrease (P < 0.05) in
survival was observed for both strains compared
with cells grown under iron replete conditions or
pulsed with peroxide. The decrease in bacterial
survival in cultures grown under iron limited
conditions suggests the presence of an ironcofactored
catalase in P. damselae subsp. piscicida.
In this way, the ability to obtain iron from the
host would determine the ability to cope with the
radicals generated during the respiratory burst. It
should also be noted that decomposition of
superoxide anions primarily generated during the
phagocytic respiratory burst depends on the activity
of a ferric superoxide dismutase in P. damselae
subsp. piscicida (Barnes et al. 1999a). Additional
studies to demonstrate the presence of iron as a
cofactor in the catalase, and the sensitivity of
P. damselae subsp. piscicida to the radicals
generated during the macrophage respiratory burst,
are in progress.
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