Download - Eppendorf

New products 2013 

Liquid Handling, Sample Handling, Cell Handling – Supplement to catalog

2 

3 

» 

Welcome to the Eppendorf 

product world « 

As a leading life sciences company, once again this year 

we’ve expanded our wide-ranging product portfolio. Our 2013 

catalog supplement offers you a variety of product innovations, 

as always with the highest level of quality. 

Within our three competence areas – Liquid Handling, Sample 

Handling and Cell Handling – we've used our expert technical 

and applications know-how to develop new products in line 

with your requirements and wishes. In line with our longstanding 

tradition, they are all designed to fulfill both our high 

standards and your expectations. 

As an example, in Liquid Handling we’ve introduced two new 

epMotion ® models, M5073 and P5073. They each offer you two 

systems in one: a tailored system for your special applications 

and a flexible, open system for automating a variety of 

applications and complex pipetting procedures. 

Since its founding 67 years ago, Eppendorf has been known as 

a premium provider of devices and consumables for laboratory 

work and research. 

Whether in academic or industrial research labs, whether 

in the food, environmental or pharmaceutical industry, 

Eppendorf’s first-class quality, comprehensive service and 

sustainability-oriented company philosophy give you the good 

feeling and confidence that you can always rely on Eppendorf 

products. 

Eppendorf – In touch with life ® 

50 years of Eppendorf tubes 

In Sample Handling, we’ve closed the gap in existing vessel 

sizes with the new 5.0 mL Eppendorf tube, for users with midsized 

sample volumes. 

Our offer in Cell Handling has been expanded too. For 

example, you’ll find the innovative Eppendorf µCuvette 

and now also the parallel DASGIP ® bioreactor systems in our 

product portfolio. 

Dr. Dirk Ehlers 

President and CEO 

To celebrate the Eppi’s 50th birthday, we're closing the gap between small and large 

volumes in our sample tubes by introducing the mid-sized 5.0 mL tube.

4 

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Index 

Table of contents 

Index 


i 

5 


Index 

INDEX 

INDEX 

Liquid Handling 8 – 23 

> > Pipetting 10 – 17 

> > Automated pipetting 18 – 23 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ADI 1025 Bio Console 83 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ez-Control 84 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & BioFlo ® / CelliGen ® 115 83 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & CelliGen ® 310 83 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Sartorius BioStat ® B-Plus (Left-Hand) 84 

Advanced rotor design – fixed-angle rotors 49 

Advanced rotor design – swing-bucket rotors 48 

Sample Handling 24 – 73 

> > Heating and mixing 26 – 37 

> > Centrifugation 38 – 57 

> > PCR 58 – 61 

> > Photometry 62 – 73 

BioBLU 0.4c Single-Use Vessel 88 

BioPhotometer D30 reference filter set 73 

BioSpectrometer fluorescence reference filter set 73 

Cell Culture Centrifuge Packages 47 

Centrifuge 5427 R 38 

Centrifuge 5430/5430 R 43 

Centrifuge 5804/5804 R and 5810/5810 R 46 

Combitips advanced ® 12 

Cell Handling 74 – 89 

> > Incubators/shakers New Brunswick 76 – 79 

> > Bioprocessing New Brunswick 80 – 85 

> > Bioprocessing DASGIP ® 86 – 89 

DASGIP ® Mini Bioreactor System DASbox ® 86 

DASGIP ® Parallel Bioreactor System for CelliGen ® BLU 5 L vessel 87 

DASware ® migrate for CelliGen ® and BioFlo ® units 89 

ep Dualfilter T.I.P.S. ® SealMax 15 

epMotion ® M5073 18 

epMotion ® M5073 and epMotion ® P5073 22 

epMotion ® P5073 20 

Eppendorf BioPhotometer ® D30 64 

Eppendorf BioSpectrometer ® fluorescence 62 

Eppendorf Easypet ® 3 16 

Eppendorf PlateReader AF2200 68 

Eppendorf SmartBlocks 36 

Eppendorf ThermoMixer C 30 

Eppendorf ThermoMixer F1.5 28 

Eppendorf ThermoMixer FP 28 

Eppendorf ThermoStat C 32 

Eppendorf ThermoTop ® 34 

Eppendorf Tubes ® 5.0 mL 26 

Eppendorf Xplorer ® plus 10 

Eppendorf µCuvette G1.0 66 

Eppendorf µPlate G0.5 72 

Title image: Eppendorf µCuvette G1.0 

The Eppendorf µCuvette G1.0 microliter measuring cell is the perfect tool for measuring high concentrations in the smallest volumes. 

More on page 66 

Product appearance, specifications, and/or prices are subject to change without notice. 

In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 

E-mail: info@eppendorf.com · www.eppendorf.com

6 

i 

Index 


Index 

Legal notices and trademark attributions 

i 

7 

Index 

INDEX 

Legal notices and trademark attributions 

INDEX 

Filter slides for PlateReader AF2200 70 

MagSep reagent kits 23 

Mastercycler ® nexus 58 

Mastercycler ® nexus flat and Mastercycler ® flat eco 61 

Mastercycler ® nexus X1 60 

New Brunswick CelliGen ® BLU 80 

New Brunswick CelliGen ® BLU Accessories 82 

New Brunswick CelliGen ® BLU Adapter Kits 83 

New Brunswick CelliGen ® BLU Adapter Kit - Op-76 Optical pH Module 84 

New Brunswick CelliGen ® BLU Single-Use Vessels 81 

New Brunswick CelliGen ® BLU Vessel Kits 82 

New Brunswick S41i 76 

Overview of the main rotors 48 

Rolling cabinet 55 

Rotor Assurance Plans 56 

Rotor F-35-48-17 54 

Rotor F-35-48-17, Rotor FA-45-20-17 and Rotor FA-45-48-11 54 

Rotor F-45-30-11 41 

Rotor F-45-48-11 41 

Rotor F-45-48-11 (new) 44 

Rotor F-45-48-5-PCR 42 

Rotor FA-45-12-17 41 

Rotor FA-45-16-17 (new) 44 

Rotor FA-45-20-17 54 

Rotor FA-45-24-11 40 

Rotor FA-45-24-11-Kit 40 

Rotor FA-45-24-11-Kit (update) 43 

Rotor FA-45-30-11 40 

Rotor FA-45-30-11 (update) 43 

Rotor FA-45-48-11 40 

Rotor FA-45-48-11 54 

Rotor FA-45-48-11 (new) 44 

Rotor S-24-11-AT 41 

Rotor S-24-11-AT (new) 44 

Rotor S-4-104 50 

Rotor S-4-72 52 

Rotors for Centrifuge 5427 R 40 

Rotors for Centrifuge 5430/5430 R 43 

Eppendorf patents 

Eppendorf is owner of numerous patents worldwide. 

Please respect our intellectual property. The following are 

those patents of Eppendorf which protect technology of 

the products described in this catalog. 

Optical sensor epMotion (U.S. Patent 6,819,437 B2) 

Calibrated 1-channel and 8-channel pipetting tools 

epMotion (U.S. patent 7,462,327) 

Disclaimers 

PCR 

In the field of PCR, Eppendorf cooperates with QIAGEN ® 

and recommends the use of QIAGEN polymerases. The 

QIAGEN Multiplex PCR Kit is intended for research 

use. No claim or representation is intended for its use 

to provide information for the diagnosis, prevention of 

treatment of a disease. 

Trademark information 

Eppendorf trademarks are recognised worldwide. Please 

respect our trademarks as we will vigorously protect their 

proper usage. The following are those trademarks of 

Eppendorf AG that are mentioned in this catalog. 

Biopur ® 

Combitips advanced ® 

Combitips ® 

Eppendorf ® 

eppendorf logo 

ep Dualfilter T.I.P.S. ® 

epMotion ® 

Eppendorf BioPhotometer ® 

Eppendorf BioSpectrometer ® 

Easypet ® 

Eppendorf Quality 

Eppendorf QuickLock ® 

Eppendorf QuickRelease 

Eppendorf SmartBlocks 

Eppendorf ThermoMixer 

Eppendorf ThermoTop ® 

UVette ® 

Eppendorf Xplorer ® 

Eppendorf µCuvette 

Mastercycler ® 

Further trademark information 

New Brunswick Scientific trademarks are recognised 

worldwide. Please respect our trademarks as we will 

vigorously protect their proper usage. The following are 

those trademarks of New Brunswick Scientific, Co., Inc., 

USA that are mentioned in this catalog. 

BioFlo ® 

CelliGen ® 

Fibra-Cel ® 

HEF ® 

Further trademarks used in this catalog 

Applikon ® 

Applikon, B.V., Netherlands 

BioStat ® Sartorius Stedim Biotech GmbH, Germany 

Corning ® 

Corning Inc., USA 

DASbox ® 

DASGIP AG, Germany 

DASGIP ® 

DASGIP Drescher Arnold & Schneider 

Aktiengesellschaft für Informationstechnologie und 

Prozeßtechnologie 

DASware ® 

DASGIP AG, Germany 

Ficoll ® 

GE Healthcare Bio-Sciences AB, Sweden 

Sartorius ® 

Sartorius AG, Germany 

Note: Eppendorf has attempted to identify the ownership 

of all trademarks from official trademark registers. Any 

omissions or errors are inadvertent. 

Product appearance, specifications, and/or prices are subject to change without notice.

8 9 

Liquid Handling 

> > Electronic pipettes 10 

> > Combitips ® 12 

> > Pipette tips 15 

> > Pipette controller 16 

> > Automated pipetting systems 18 

> > Reagent kits 23 

Eppendorf has been a trailblazer in liquid handling for over 50 years … 

In 1961, Eppendorf launched the first piston-stroke pipette. Today, our broad product offerings in 

liquid handling range from manual pipettes to electronic pipettes, dispensers and burets to automated 

pipetting systems. Eppendorf products are associated with state-of-the-art technology, outstanding 

ergonomics and award-winning design. This applies to both devices and the requisite consumables 

such as pipette tips and Combitips ® .



10 11 

Pipetting 

Pipetting 

Eppendorf Xplorer ® plus 

Intelligent, preset 

applications: aspiration, 

dilution, sequential 

dispense and pipetting, 

reverse pipetting 

The Eppendorf Xplorer 

plus offers all Eppendorf 

Xplorer functions 

including an intuitive 

operating concept 

Pipetting 

Pipetting 

Pipetting 

Individual speed setting 

allows you to set 

parameters at a speed 

you are comfortable with 

One-time programming of 

individual sequences for 

simple editing of complex 

processes 

History function: 

automatic storage of last 

10 parameter settings 

(can be programmed 

as an alternative to the 

Help function) 

Reminder function for 

pipette servicing, based 

on time or usage interval 

Description 

Protecting resources, saving time and guaranteed precision 

are the key aspects of all laboratory work. The Eppendorf 

Xplorer plus was developed to exceed these high standards. 

Based on the Eppendorf Xplorer, this expanded version offers 

exciting extras such as intelligent applications, adjustable fixed 

volumes, individual programming and a history function to 

make work much faster and easier. The Eppendorf Xplorer plus 

is the perfect choice for all users who need advanced speed, 

safety and reliability every day. 

Applications 

> > Pipetting liquids (manual and electronic) 

> > Dispensing (timed and manual) 

> > Pipetting and mixing 

> > Diluting 

> > Aspirating 

> > Sequential dispensing and pipetting 

> > Reverse pipetting 

> > Volume determination in manual pipetting mode 

> > Filling of plates, gels, and reaction vessels 

> > Phase extraction and removal of supernatants 

Pipetting 

Pipetting 

Storage of frequently 

used fixed volumes 

Programming and 

adjustment protection via 

an optional password 

Ordering information 

Volume range Color code Catalog No. List USD 

Eppendorf Xplorer ® plus, single-channel 

0.5 – 10 µL Medium grey 4861000708 $ 920.00 

5 – 100 µL Yellow 4861000716 920.00 

15 – 300 µL Orange 4861000724 920.00 

50 – 1,000 µL Blue 4861000732 920.00 

0.25 – 5 mL Violet 4861000740 920.00 

0.5 – 10 mL Turquoise 4861000759 920.00 

Eppendorf Xplorer ® plus, 8-channel 

0.5 – 10 µL Medium grey 4861000767 1,570.00 

5 – 100 µL Yellow 4861000783 1,570.00 

15 – 300 µL Orange 4861000805 1,570.00 

50 – 1,200 µL Green 4861000821 1,570.00 

Eppendorf Xplorer ® plus, 12-channel 

0.5 – 10 µL Medium grey 4861000775 1,980.00 

5 – 100 µL Yellow 4861000791 1,980.00 

15 – 300 µL Orange 4861000813 1,980.00 

50 – 1,200 µL Green 4861000830 1,980.00 

Pipetting 

Order from your laboratory supply dealer. 






12 13 

Dispensing 

Dispensing 


Dispensing 

Dispensing 

Dispensing 

New! Dispenser box with extricable chute for easy access 

and storage 

Compatibility of CBTA with Standard Laboratory Vessels 

Combitip rack supports single-hand operation and use in 

sterile environment 

Dispensing 

Dispensing 

Description 

The Eppendorf Combitips have been completely redesigned 

and optimized to meet the increased requirements of a modern 

laboratory to a greater extent than ever before. The result 

is the Combitips advanced. As they offer a wide variety of 

advantages in comparison with the Combitips plus and other 

dispenser tips, we talk about the 360° Evolution! 

The Combitips advanced also function according to the positive 

displacement principle. Thus they always dispense the correct 

volume independently of the density of the liquid and its flow 

characteristics (e.g. increased vapor pressure or increased 

viscosity). Working with radioactive, or toxic materials also 

becomes safer due to the hermetically sealed piston preventing 

aerosol contamination. 

Product features 

> > Combitips advanced are the ideal system components for all 

Eppendorf Repeaters 

> > The 9 volume sizes (0.1 mL – 50 mL) offer a maximum range 

of volumes 

> > Individual dispensing volumes from 1 µL to 50 mL 

> > Elongated Combitips (2.5 mL, 5.0 mL, 10 mL) make it 

possible to reach the bottom of standard laboratory tubes 

(see chart on right.) 

> > Individual color coding facilitates quick identification of the 

desired Combitips 

> > The funnel geometry prevents damage to gloves and ensures 

comfortable handling 

> > The new Combitip Rack features optimal single-hand 

operation 

> > With the new dispenser box (with extricable chute) the 

Combitips are always within reach 

> > The appropriate purity grade for each application: Eppendorf 

Quality, Sterile or Eppendorf Biopur ® 

Eppendorf Quality: 

> > Portioning in 4 bags (25 pcs. each) ensures dust-free storage 

Sterile and Biopur Quality: 

> > Individually wrapped (Biopur supports clean room conditions) 

Combitips 

Eppendorf Tubes ® Conical Tubes Eppendorf Plates ® Deepwell 

advanced ® 0.5 mL 1.5 mL 2.0 mL 5.0 mL 15 mL 50 mL 96/500 µL 96/1000 µL 96/2000 µL 

0.1 mL + + + + - - + + + 

0.2 mL + + + + - - + + + 

0.5 mL + + + + - - + + + 

1.0 mL + + + + - - + + + 

2.5 mL + 1 + 1 + 1 + + 1 + 1 + + 1 + 1 

5.0 mL + + + + + 1 + 1 + + + 

10 mL + + + - - + 1 + + + 

25 mL - + + - - - + + + 

50 mL - + + - - - + + + 

+ 1 = improved compatibility compared to Combitip plus generation 

Dispensing 







14 15 

Dispensing 

Pipetting 


ep Dualfilter T.I.P.S. ® SealMax 


Description Color Code Catalog No. List USD 

Combitips advanced ® , 0.1 mL 

Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) White 0030089405 $113.00 

Biopur, 100 pcs., individually wrapped White 0030089618 161.00 

Sterile, 100 pcs., individually wrapped White 0030089510 142.00 


Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Light blue 0030089413 113.00 

Biopur, 100 pcs., individually wrapped Light blue 0030089626 161.00 

Sterile, 100 pcs., individually wrapped Light blue 0030089529 142.00 


Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Purple 0030089421 113.00 

Biopur, 100 pcs., individually wrapped Purple 0030089634 161.00 

Sterile, 100 pcs., individually wrapped Purple 0030089537 142.00 


Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Yellow 0030089430 113.00 

Biopur, 100 pcs., individually wrapped Yellow 0030089642 161.00 

Sterile, 100 pcs., individually wrapped Yellow 0030089545 142.00 


Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Green 0030089448 113.00 

Biopur, 100 pcs., individually wrapped Green 0030089650 161.00 

Sterile, 100 pcs., individually wrapped Green 0030089553 142.00 


Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Blue 0030089456 113.00 

Biopur, 100 pcs., individually wrapped Blue 0030089669 161.00 

Sterile, 100 pcs., individually wrapped Blue 0030089561 142.00 

Combitips advanced ® , 10 mL 

Eppendorf Quality, 100 pcs. (4 bags x 25 pcs.) Orange 0030089464 113.00 

Biopur, 100 pcs., individually wrapped Orange 0030089677 161.00 

Sterile, 100 pcs., individually wrapped Orange 0030089570 142.00 


Eppendorf Quality, 100 pcs. (4 boxes x 25 pcs.) Red 0030089472 142.00 

Biopur, 100 pcs., individually wrapped Red 0030089685 184.00 

Sterile, 100 pcs. (4 boxes x 25 pcs.), individually wrapped Red 0030089588 167.00 


Eppendorf Quality, 100 pcs. (4 boxes x 25 pcs.) Light grey 0030089480 142.00 

Biopur, 100 pcs., individually wrapped Light grey 0030089693 184.00 

Sterile, 100 pcs. (4 boxes x 25 pcs.), individually wrapped Light grey 0030089596 167.00 

Combitips advanced ® Rack, for 8 Combitips advanced (0.1 – 10 mL) 0030089758 39.50 

Adapter advanced, 25 mL, autoclavable, 1 pcs. Red 0030089715 21.00 

Adapter advanced, 25 mL, autoclavable, Biopur, 7 pcs., individually wrapped Red 0030089731 127.00 

Adapter advanced, 50 mL, autoclavable, 1 pcs. Light grey 0030089723 21.00 

Adapter advanced, 50 mL, autoclavable, Biopur, 7 pcs., individually wrapped Light grey 0030089740 127.00 

Dispensing 

Dispensing 

Dispensing 

Description 

The new violet/white filter for the ep Dualfilter T.I.P.S. ® 

SealMax filter tips combine reliable pipette protection 

from contaminating contact with the sample due to 

overpipetting with virtually 100 % protection from aerosols 

and biomolecules. This innovative filter is the first Dualfilter 

which immediately closes when it comes into contact with 

the sample, without inhibiting the sample or losing valuable 

sample material. The pipetting procedure can continue without 

wasting time or discarding the tip with the valuable sample. 

ep Dualfilter T.I.P.S. SealMax filter tips are manufactured from 

pure, non-recycled materials, made of the highest quality, 

under clean room conditions. They are available in tested PCR 

clean/Sterile (sterile and pyrogen-free) purity grade. Batchspecific 

certification is provided by an independent laboratory. 

* 1 Aerosol: Aerosol particles and the air or dispersion of solid or liquid particles surrounding it 

in a gas, usually air. 


> > Reliable protection against liquids, aerosols and biomolecules 

> > Contamination-free sample recovery also after prolonged 

contact with liquid 

> > Sample recovery via simple dispensing without replacing the 

tip or pipette 

> > Tips are free of PCR inhibitor additives, meaning that no 

valuable samples will need to be discarded 

> > Protects the pipette from aggressive liquids even if liquid level 

in tip is too high 

> > Available in PCR clean/Sterile (sterile and pyrogen-free) 

> > Batch-specific certificates are available online at: www. 

eppendorf.com/certificates. 


Description Color Code Catalog No. List USD 

ep Dualfilter T.I.P.S. ® SealMax, PCR clean, Sterile 

0.1 – 10 µL S, 34 mm, Dark grey, 960 tips (10 racks x 96 tips) Dark grey 0030077806 Inquire 

0.5 – 20 µL L, 46 mm, Light grey, 960 tips (10 racks x 96 tips) Light grey 0030077814 Inquire 

2 – 100 µL, 53 mm, Yellow, 960 tips (10 racks x 96 tips) Yellow 0030077822 Inquire 

2 – 200 µL, 55 mm, Yellow, 960 tips (10 racks x 96 tips) Yellow 0030077830 Inquire 

20 – 300 µL, 55 mm, Orange, 960 tips (10 racks x 96 tips) Orange 0030077849 Inquire 

50 – 1,000 µL, 76 mm, Blue, 960 tips (10 racks x 96 tips) Blue 0030077857 Inquire 

Pipetting 

Pipetting 

Pipetting 







16 17 

Pipetting 

Pipetting 

Eppendorf Easypet ® 3 

PipettING 

Electronic Pipet Helper 

® 


® 

PipettING 

Description 

Experience a new dimension of electronic pipetting with 

the new Eppendorf Easypet ® 3. The latest technological 

developments give you complete speed control with the utmost 

precision. The lightweight, well-balanced Easypet 3 has been 

designed considering all ergonomic findings to sit comfortably 

in your hand and matches its shape to allow for fatigue-free 

pipetting. 

Applications 

> > Pipette controller for use with pipettes from 0.1 - 100 mL 

> > Dispensing of aliquots of various volumes 

> > Resuspension of bacterial or cell pellets 

> > Aspiration of cell layer from, e.g., Ficoll ® gradient 


> > Intuitive and convenient speed adjustment made simple with 

the tips of your fingers 

> > Lightweight, well-balanced and ergonomic design that allows 

for fatigue-free pipetting 

> > Vibrant backlit LEDs provide optical feedback of the 

remaining battery life 

> > Lithium polymer rechargeable battery offers extended 

cordless runtime 

> > Smooth setting of pump speed 

> > Operation while recharging is possible 

> > Autoclavable pipette adapter for sterile applications 

> > Quick release of aspirating cone for easy exchange of 

membrane filters 

Lightweight, well-balanced and ergonomic design ensures 

fatigue-free pipetting. 

Technical specifications 

Weight 

Rechargeable battery 

Capacity 

Charging time 

Type 

Number of dispensings 

Mains/power supply 

Input 

Output 

160 g 

Battery status display now on backlit LEDs for your own peace 

of mind. 

1,100 mAh/3.7 V 

~3 h 

Lithium polymer 

~2,000 (with a 25 mL pipette) 

100 V – 240 V ± 10 %, 50 Hz – 60 Hz, 0.5 A 

5 V DC, 1.0 A 


Description Catalog No. List USD 

Eppendorf Easypet ® 3, includes mains/power supply device, wall mounting device, 

4430000026 $442.00 

2 membrane filters 0.45 µm 

Membrane filter, for Easypet 3 and Easypet 4421, 0.45 µm, sterile, PTFE, 5 pcs. 4421601009 119.84 

Membrane filter, for Easypet 3 and Easypet 4421, 0.2 µm, sterile, PTFE, 5 pcs. 4430606005 119.84 

Li-polymer battery, for Easypet 3 4430605009 34.30 


® 


® 







18 19 

Automated pipetting 


epMotion ® M5073 





Description 

The epMotion M5073: 2 systems in one. First, a customized 

system for automated nucleic acid purification of 1 to 24 

samples with special MagSep reagents. The Eppendorf 

EasyCon control panel and Prep Assistant software provide 

simple operation with step-by-step guidance through the 

process. Secondly, a flexible system for automating timeconsuming 

and complex pipetting procedures with the epBlue 

software, making routine liquid handling tasks easier than ever. 

The pipetting procedure is more precise, reproducible and fully 

standardized, making your workplace more ergonomic and 

safer. The epMotion M5073 is delivered with all accessories 

required for nucleic acid purification; only consumables need 

to be ordered separately. 

Applications 

> > DNA and RNA purification 

> > PCR set-up 

> > Sample or reagent transfer 

> > Sample mixing and tempering 

> > Assay set-up 

> > Media change and other cell culture applications 


> > 6 ANSI/SBS microplate positions 

> > Compact control panel with touchscreen or mouse operation, 

USB data transfer 

> > Software assistants for simple, step-by-step application 

programming 

> > Optical sensor* 1 for detecting liquids, labware, tips 

> > Automatic replacement of 2 pipetting tools 

> > Volume range of 1 to 1,000 μL for maximum pipetting 

accuracy 

> > 3D-MagSep technology, mixing, tempering, and magnetic 

separation all at one location 

> > Calibrated 1-channel and 8-channel pipetting tools* 2 

> > Optional UV lamp and HEPA filter system for decontamination 

and clean air inside 

> > Compatible with tubes (0.1 μL to 50 mL) and microplates with 

up to 384 wells 

* 1 US patent 6,819,437 B2 

* 2 US patent 7,462,327 

3D MagSep technology 

Unique combintation of magnet finger module and TMX 

Thermomixer allow you to separate, mix and heat/cool in 

one position. 

New innovative functions 

The new Liquid Waste Tub allows you to easily separate liquid 

and solid waste. 

And what‘s more, you can now save tips with the reuse tips 

feature. 



epMotion ® M5073, for automated nucleic acid preparation from 1 – 24 samples with 

5073000205 Inquire 

Eppendorf MagSep kits, 100 – 240 V/50 – 60 Hz, with control panel, epBlue software with 

Prep Assistant, TS 50, TS 1000, PrepRack, ReagentRack, Rack24 accessories 

epMotion ® M5073c, for automated nucleic acid preparation from 1 – 24 samples with 

Eppendorf MagSep kits, 100 – 240 V/50 – 60 Hz, with CleanCap, control panel, epBlue 

software with Prep Assistant, TS 50, TS 1000, PrepRack, ReagentRack, Rack24 

5073000400 Inquire 



Notice to purchaser; limited license for research use only 

This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The purchase price for this product includes only limited, nontransferable rights under certain 

claims of certain patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights are conveyed. Purchaser is not granted any rights under patents 

of Gen-Probe Incorporated to use this product for any commercial use. Further information regarding purchasing a license under patents of Gen-Probe Incorporated to use this product for any 

other purposes, including, without limitation, for commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development Department, 10210 Genetic Center Drive, 

San Diego, California 92121-4362, U.S.A. 

Order direct from Eppendorf North America. 






20 21 



epMotion ® P5073 





Description 

The epMotion P5073: 2 systems in one. First, a customized 

system for automatic PCR preparation in the 96 or 384 plate 

formats. The Eppendorf EasyCon control panel and PCR 

Assistant software provide simple operation with step-bystep 

guidance through various PCR preparations. Secondly, a 

flexible system for automating time-consuming and complex 

pipetting procedures with the epBlue software, making routine 

liquid handling tasks easier than ever. The pipetting procedure 

is more precise, reproducible and fully standardized, making 

your workplace more ergonomic and safer. The epMotion 

P5073 is delivered with all accessories required for preparing 

PCR samples; only consumables need to be ordered separately. 

Applications 

> > PCR set-up 

> > Serial dilution and normalization of samples 

> > Sample or reagent transfer 

> > Sample mixing and tempering 

> > Assay set-up 

> > Media change and other cell culture applications 


> > 6 ANSI/SBS microplate positions 

> > Compact control panel with touchscreen or mouse operation, 

USB data transfer 

> > Software assistants for simple, step-by-step application 

programming 

> > Optical sensor* 1 for detecting liquids, labware, tips 

> > Automatic replacement of 2 pipetting tools 

> > Volume range of 1 to 1,000 μL for maximum pipetting 

accuracy 

> > Compatible with tubes (0.1 μL to 50 mL) and microplates with 

up to 384 wells 

> > Calibrated 1-channel and 8-channel pipetting tools* 2 

> > Optional UV lamp and HEPA filter system for decontamination 

and clean air inside 

> > Fully enclosed, compact housing with door lock 

* 1 US patent 6,819,437 B2 

* 2 US patent 7,462,327 

More flexibility 

The 6 positions of the epMotion 5073 systems, automatic 

tool change, and gripper option open up a wide variety of 

applications. 

Simple and fast operation via software assistants 

Easily create PCR mastermixes and dilution series 

for quantification standards, normalization of sample 

concentrations and setting up PCR reactions – using 

touchscreen or mouse operation. 



epMotion ® P5073, for automatic preparation of PCR reactions, 100 – 240 V/50 – 60 Hz, 

5073000000 Inquire 

with control panel, epBlue software with PCR Assistant, TS 50, TS 300, thermoblock, 

Rack24 accessories 

epMotion ® P5073c, for automatic preparation of PCR reactions, 100 – 240 V/50 – 60 Hz, 

with CleanCap, control panel, epBlue software with PCR Assistant, TS 50, TS 300, 

thermoblock, Rack24 accessories 

5073000302 Inquire 









22 23 



epMotion ® M5073 and epMotion ® P5073 

MagSep reagent kits 





Dimensions (W × D × H) 

epMotion M5073 epMotion M5073c epMotion P5073 epMotion P5073c 

65 × 61 × 67 cm/ 

25.6 x 24.0 x 26.3 in 

65 × 61 × 77 cm/ 

25.6 x 24.0 x 30.3 in 

65 × 61 × 67 cm/ 

25.6 x 24.0 x 26.3 in 

65 × 61 × 77 cm/ 

25.6 x 24.0 x 30.3 in 

Weight w/o accessories 57 kg / 125.6 lb 62 kg / 136.7 lb 50 kg / 110.2 lb 55 kg / 121.3 lb 

Power supply 100 – 240 V ±10 %, 50 – 60 Hz ± 5 % 

Max. power consumption 

600 W 

Eppendorf EasyCon 

Display 

LCD color display 

Display resolution 

1,024 × 600 pixels 

Interfaces 4 × USB 2.0 

Touch panel 

Capacitive touch panel 

Gripper carrying capacity 

1200 g 

HEPA filter 

Filter class according to EN 1822 E 10 E 10 

Volume flow 70 – 80 m 3 /h 70 – 80 m 3 /h 

Noise level ~ 56 dB(A) ~ 56 dB(A) HEPA 

off 

Optical confocal infrared detector 

Detection conditions 

Positioning in MTP format 6 

Thermomixer 

Max. load 

1,000 g (2.2 lb) 

Mixing period 

5 s – 120 min 

Speed 

300 rpm – 2,000 rpm 

Temperature range 4 °C – 95 °C 

Thermal module (optional) 

Cooling rate 

Heating rate 

~ 56 dB(A) ~ 56 dB(A) HEPA 

off 

Contact-free recognition of fill level of inserted tools, labware, tip types and quantities 

Liquid surface 90 ± 3° to the optical axis, minimum level 3 mm 

4 °C/min 

9 °C/min 

Temperature range 0 °C – 110 °C 

UV lamp 

Irradiation period per run 15 min 15 min 

Radiation capacity at a distance of 

39 μW/cm 2 39 μW/cm 2 

1 m 

Radiation capacity after 100 h 3,9 W 3,9 W 

Main wavelength 254 nm (UV-C) 254 nm (UV-C) 

X-Y-Z axis positioning accuracy 

Systematic deviation 

±0.1 mm 

Random deviation 

±0.3 mm 

Description 

MagSep reagent kits are used with the epMotion M5073 to 

automatically purify nucleic acids from biological samples. 

Magnetic beads, Proteinase K and all other reagents are 

pre-prepared in special trays for the epMotion for 4 sets of 

24 purifications. MagSep reagents deliver highly purified 

nucleic acids with very high yields. The special Prep Assistant 

software, combined with the epMotion M5073, offer simple, 

step-by-step guided operation through automatic nucleic acid 

purification for everyday use. 

Applications 

> > Genomic DNA from tissue and cell culture or bacteria - 

MagSep Tissue gDNA kit 

> > Genomic DNA from 200 uL whole blood - MagSep Blood 

gDNA kit 

> > Viral nucleic acids from serum, plasma or other cell-free body 

fluids - MagSep viral DNA/RNA kit 


> > Higher yields compared to manual purifications 

> > High nucleic acid purity, suitable for PCR or other enzymatic 

reactions 

> > Automatic recognition of kit type and remaining sample 

quantity 

> > Variable use for 1 to 24 samples per tray (interim storage at 

4 °C) 

> > Minimal preparation time due to special trays 

> > Elution in DNA LoBind vessels (supplied) 

> > Storage at room temperature 






MagSep Tissue gDNA Kit, reagent kit for genomic DNA purification of 4 × 24 samples 

0030450000 Inquire 

from cells & tissues 

MagSep Blood gDNA Kit, reagent kit for genomic DNA purification of 4 × 24 samples 

0030451007 Inquire 

from blood 

MagSep Viral DNA/RNA Kit, reagent kit for viral DNA/RNA purification of 4 × 24 samples 0030452003 Inquire 





24 25 

Sample Handling 

> > Reaction tubes 26 

> > Mixers and temperature control systems 28 

> > Centrifugation 38 

> > PCR thermocycler 58 

> > Photometry 62 

Technology leader in sample handling for 60 years … 

Eppendorf products set standards in a wide variety of laboratory areas at an early stage – standards that 

still serve as yardsticks for others today. Sample handling encompasses many different work processes 

and steps: centrifugation, heating, freezing, mixing, amplification, and analysis of samples. Eppendorf 

offers the devices and consumables needed for each of these steps and allows users to feel assured that 

the work they perform is of the highest quality.



26 27 

Heating & Mixing 


Eppendorf Tubes ® 5.0 mL 

40 

35 




Ergonomic operation: 

Simple, practical and 

ergonomic single-hand 

operation. Large labeling 

area. 

Certified purity: Available in 

batch-tested and certified 

Eppendorf Quality, PCR 

clean, Sterile and Eppendorf 

Biopur ® purity levels 

Centrifugation safety: 

Maximum safety and 

stability for centrifugation 

up to 25,000 x g. Fast and 

efficient protocols. 

Description 

Eppendorf Tubes are the benchmark for simple and safe 

sample preparation from 0.5 to 2.0 mL. For larger volumes, 

15 mL and 50 mL conical tubes are available. For medium 

volumes, between 2 and 15 mL you may face an issue: they 

need to be processed with large conical screw cap tubes - 

impractical, inconvenient and often prone to contamination. 

Eppendorf now offers the »missing link«: the Eppendorf Tube 

5.0 mL. This product fills the gap between existing tube 

versions and enables the simple and safe processing of 

sample volumes up to 5.0 mL! 

www.eppendorf.com/5ml 

www.eppendorf.com/consumables 

Lid design: Hinged lid 

for minimized sample 

evaporation during storage 

and incubation in a wide 

range of temperatures from 

–86 °C to 80 °C. 

Material: Exceptionally 

high-quality, transparent 

polypropylene, free of 

plasticizers, biocides or mold 

release agents, for reliable 

test results. 

Peace of mind: Compatible 

with accessories for conical 

15 mL tubes - many existing 

adapters and racks can be 

used. 

Applications 

> > Easier processing of samples up to 5.0 mL 

> > Higher yields in DNA isolation, especially with samples with 

limited quantity of source material 

> > Convenient and safe cell culture applications 

> > Easier sample access lowers the risk of contamination 

> > Preparation of mastermixes and buffers 

> > Less storage space needed 

> > Safe cell and tissue lysis 

New, matching high-speed rotors and adapters for 

centrifugation, thermoblocks for mixing and incubation, 

racks and boxes for sample preparation and storage. 

Yield DNA [μg] 

30 

25 

20 

15 

10 

5 

0 

1,5 mL 2,0 mL 5,0 mL 

Eppendorf Tube 

Higher yields in DNA isolation – e.g. the yield of low-copy 

plasmid DNA can be significantly improved by increasing 

the culture volume to 5 mL. For additional information and 

protocol description please see application note 262. 



Eppendorf Tube ® 5.0 mL, Eppendorf Quality, 200 tubes (2 bags of 100 tubes) 0030119401 $ 51.00 

Eppendorf Tube ® 5.0 mL, PCR clean 1 , 200 tubes (2 bags of 100 tubes) 0030119460 54.00 

Eppendorf Tube ® 5.0 mL, Sterile 2 , 200 tubes (10 bags of 20 tubes) 0030119487 56.00 

Eppendorf Tube ® 5.0 mL, Biopur ®3 , 50 tubes (individually wrapped) 0030119479 57.00 

Eppendorf Protein LoBind Tube 5.0 mL, PCR clean1, 100 tubes (2 bags of 50 tubes) 0030108302 51.00 

Eppendorf DNA LoBind Tube 5.0 mL, PCR clean1, 200 tubes (4 bags of 50 tubes) 0030108310 61.00 

Accessories for general use 

Eppendorf Tube ® 5.0 mL Starter Pack, 400 tubes in PCR clean 1 purity (4 bags of 100 0030119380 165.00 

tubes), 2 Tube Racks, 8 Universal inserts 

Tube Rack for 16 x 5.0 mL, also suitable for 15 mL conical screw cap tubes, white, 2 pcs. 0030119495 51.00 

Storage Box for 25 x 5.0 mL, polypropylene, with lid and alphanumeric code, suitable for 0030127943 23.00 

-86°C storage, height 65 mm, 2 pcs. (blue and red) 

Accessories for Liquid Handling 

Eppendorf Research plus 5.0 mL, single-channel pipette, 0.5-5.0 mL, Violet 3120000070 348.00 

Eppendorf Xplorer 5.0 mL, single-channel electronic pipette, 0.25-5.0 mL, Violet 4861000058 730.00 

epT.I.P.S. 0.1-5.0 mL, Eppendorf Quality, 500 tips (5 bags of 100 tips) 022492080 92.50 

epT.I.P.S. 0.1-5.0 mL, Eppendorf Quality, 1 reusable box (24 tips) 022491385 38.00 

ep Dualfilter T.I.P.S. 0.1-5.0 mL, PCR Clean and Sterile, 120 tips (5 racks of 24 tips) 022491261 78.00 

Eppendorf Reservoir Rack Module TC 4 x 5.0 mL, for use in epMotion ® 5075799340 413.00 

Accessories for Centrifuges 

Universal insert, for use of Eppendorf Tube 5.0 mL in centrifuge rotors and adapters with 5820732008 28.00 

15 mL conical bore holes, set of 8 pcs. 

Universal adapter 1 x 5.0 mL, for rotors with 50 mL concial bore hole, set of 2 pcs. 5820730005 155.00 

Universal adapter 1 x 5.0 mL, for rotors with 100 mL round bore hole, set of 2 pcs. 5804777000 155.00 

Eppendorf adapter 1 x 5.0 mL, for rotor 6 x 15/50 mL, Centrifuge 5430/5430 R, sm. bore, 2 pcs. 5427746005 145.00 

Eppendorf adapter 1 x 5.0 mL, for rotor 6 x 15/50 mL, Centrifuge 5430/5430 R, lg. bore, 2 pcs. 5427747001 145.00 

Eppendorf adapter 1 x 5.0 mL, for 4 x 100 mL rotor, Centrifuge 5702/5702 R/5702 RH, 2 pcs. 5702733008 155.00 

Eppendorf adapter 8 x 5.0 mL, for 4 x 250 mL rotor, Centrifuge 5804/5804 R/5810/5810 R, 2 pcs. 5804793005 270.00 

Eppendorf adapter 14 x 5.0 mL, for 4 x 750 mL rotor of Centrifuge 5810/5810 R, set of 2 pcs. 5825739000 290.00 

Eppendorf rotor 12 x 5.0 mL for Centrifuge 5427 R (FA-45-12-17), max. 20,600 x g, with 5409700006 970.00 

aerosol-tight Eppendorf QuickLock ® lid 

Eppendorf rotor 16 x 5.0 mL for Centrifuge 5430/5430 R (FA-45-16-17), max. 21,191 x g, 5427750002 1,070.00 

with aerosol-tight Eppendorf QuickLock ® lid 

Eppendorf rotor 20 x 5.0 mL for Centrifuge 5804/5804 R and 5810/5810 R (FA-45-20-17), 5820765003 1,670.00 

max. 20,913 x g, with aerosol-tight Eppendorf QuickLock ® lid 

Accessories for Eppendorf Thermomixer and ThermoStat 

Eppendorf Thermoblock 8 x 5.0 mL, for Eppendorf Thermomixer R and ThermoStat plus 5309000333 708.00 

Eppendorf SmartBlock 8 x 5.0 mL, for Eppendorf ThermoMixer C and ThermoStat C 5309000007 710.00 

1 PCR clean: Batch-certified free from DNA, DNase, RNase, and PCR inhibitors. 2 Sterile: Batch-certified sterile. 

3 Biopur: Batch-certified sterile and free from DNA, DNase, RNase, PCR inhibitors, ATP and pyrogens/endotoxins. 










28 29 



Eppendorf ThermoMixer F1.5 

and Eppendorf ThermoMixer FP 





Description 

To simplify your lab routines. Whether you regularly work 

with 1.5 mL reaction vessels or plates (MTP and DWP) - the 

customized systems from Eppendorf offer you the perfect 

solution for your special applications. The devices combine 

precise heating and mixing with the highest stability, even 

at maximum mixing frequency. You can also carry out 

independent mixing and incubation steps. 

Applications 

> > Growth of bacteria and yeasts 

> > Enzymatic reactions 

> > Transformation of bacteria strains and plasmids 

> > Denaturation of DNA, RNA and proteins 

> > Lysis reactions at 100°C 

> > Labeling of nucleic acids and proteins 

> > Proteinase K digestion of cells and tissues 

> > Resuspension of pellets 

> > Mixing protein quantification assays 

> > ELISA assays 


> > Heating and mixing in 1.5 mL reaction vessels (F1.5) or 

plates (FP) 

> > Efficient mixing up to 1,500 rpm (F1.5) or 2,000 rpm (FP) 

> > Excellent mixing performance due to unique 2D Mix-Control 

technology 

> > Anti-spill technology prevents lid wetting and crosscontamination 

> > Reliable prevention of condensate formation with the optional 

ThermoTop (condens.protect) lid (see page 35 for more info) 

> > Simple and intuitive operation using predefined temperature 

keys (37 °C, 42 °C, 56 °C and 95 °C) 

> > Very quiet operation 

> > Heat-insulated SmartBlocks for ergonomic operation - you 

won‘t burn your fingers 


ThermoMixer F1.5 

ThermoMixer FP 


20.6 × 30.4 × 13.1 cm / 8.1 x 12.0 x 5.2 in 

Weight w/o accessories 6.0 kg / 13.2 lb 5.8 kg / 12.8 lb 

Power supply 

120 V, 50 – 60 Hz, 230 V, 50 – 60 Hz 


200 W 

Interfaces 

USB interface 

Temperature accuracy ±0.5 °C at 20– 45 °C ±1 °C at 20– 45 °C 

Temperature control range From 4 °C above RT to 100 °C 

Temperature setting range 1 °C to 100 °C 

Timer 

Not available 



Eppendorf ThermoMixer F1.5, with thermoblock for 24 reaction vessels 1.5 mL, 

5384000020 $2,700.00 

100 – 130 V/50 – 60 Hz 

Eppendorf ThermoMixer FP, with thermoblock for microplates and deepwell plates, 5385000024 2,930.00 

including lid, 100 – 130 V/50 – 60 Hz 

Eppendorf ThermoTop ® , with condens.protect technology 5308000003 625.00 









30 31 



Eppendorf ThermoMixer C 





Description 

Sample preparation redefined. Improve your assay results 

by mixing and incubating samples at the same time. The 

ThermoMixer C convinces with its quick and easy-to-exchange 

SmartBlocks for all common lab vessels, new and improved 

program functions, and high level of stability for every mixing 

frequency. There is no limit to the variety of applications 

waiting for you! 

Applications 

> > Plasmid/RNA/DNA purification 

> > cDNA synthesis 

> > Extraction of DNA fragments from agarose gels 

> > Enzymatic reactions (e.g., DNA restriction digestion, 

Proteinase K digestion and ligation) 

> > Transformation of bacteria strains and plasmids 

> > Denaturation of DNA, RNA and proteins 

> > Labeling of DNA, RNA and proteins 

> > Bacterial growth in microreaction vessels, conical tubes and 

deepwell plates 

> > Lysis reactions at 100 °C 

> > Mixing of PCR setups 


> > Heating, mixing and cooling in all common vessel and plate 

formats from 5 µL to 50 mL 

> > High-speed mixing up to 3,000 rpm (depending on the 

SmartBlock used) for fast mixing in even 384-well plates 

> > Excellent mixing performance due to unique 2D Mix-Control 

technology 

> > Anti-spill technology prevents lid wetting and crosscontamination 



> > Excellent temperature management and maximum 

temperature accuracy 

> > Maximum flexibility due to large selection of exchangeable 

SmartBlocks (see page 36 for more info) 

> > Simple and intuitive operation due to predefined program 

keys and clearly arranged menu 

> > Versatile program functions 

> > Ergonomic design and optimized features according to the 

PhysioCare Concept 

> > Saves up to 20 user-defined programs 

> > 2 second block exchange due to quick release system of 

SmartBlocks 



Weight w/o accessories 

Power supply 


Interfaces 

Max. speed 

Temperature accuracy 

20.6 × 30.4 × 13.1 cm / 8.1 x 12.0 x 5.2 in 

6.2 kg / 13.7 lb 

120 V, 50 – 60 Hz, 230 V, 50 – 60 Hz 

200 W 

USB interface 

3,000 1) rpm 

±0.5 °C at 20-45 °C, 

±1.0 °C below 20 °C and above 45 °C 

Temperature control range From 15 °C (±2 °C) below RT to 100 °C 

Temperature setting range 1 °C to 100 °C 

Timer 

15 sec to 99:30 h, continuous 

1) 

depends on SmartBlock used 



Eppendorf ThermoMixer C, basic device without thermoblock, 100 – 130 V/50 – 60 Hz 5382000023 $3,570.00 


Eppendorf SmartBlock 0.5 mL, thermoblock for 24 reaction vessels 0.5 mL 5361000031 710.00 



Eppendorf SmartBlock 5.0 mL, thermoblock for 8 Eppendorf Tubes 5.0 mL 5309000007 710.00 

Eppendorf SmartBlock 15 mL, thermoblock for 8 conical tubes 15 mL 5366000021 710.00 


Eppendorf SmartBlock cryo, thermoblock for 24 cryo tubes, max. 12.5 mm diameter 5367000025 710.00 

Eppendorf SmartBlock 12 mm, thermoblock for 24 tubes, max. 12 mm diameter, 

5364000024 710.00 

height 30 mm – 76 mm 

Eppendorf SmartBlock plates, thermoblock for MTPs and deepwell plates, incl. lid 5363000039 940.00 

Eppendorf SmartBlock PCR 96, thermoblock for PCR plates 96, incl. lid 5306000006 940.00 










32 33 

Temperature control 

Temperature control 

Eppendorf ThermoStat C 

Temperature COntROL 




Description 

The ideal device to accurately set and maintain temperatures. 

The ThermoStat C features excellent temperature 

management. Peltier technology provides the comfortable 

and precise temperature control of all conventional lab 

vessels. The operation is easier and more intuitive than 

ever before. Predefined temperature keys provide quick 

access to five important experimental temperatures. Due to 

comprehensive program functions, you can also individually 

store 15 programs. With an extensive range of exchangeable 

SmartBlocks, the ThermoStat C adapts to any application. 

Applications 

> > Gentle thawing and cooling buffers and samples at low 

temperatures 

> > Preheating media 

> > Biochemical reactions at low temperatures 

> > Ligation 

> > cDNA synthesis 

> > Denaturation of RNA, DNA and protein 

> > Proteinase K digestion 

> > ELISA assays 

> > Immunoprecipitation 

> > Cell lysis 


> > Heating and active cooling in all common vessel and plate 

formats from 5 µL to 50 mL 

> > Excellent temperature management 

> > Precise temperature control from -10 °C to 110 °C * 1 

> > Precise maintenance of various temperature levels 



> > Maximum flexibility thanks to large selection of exchangeable 

SmartBlocks (see page 36 for more info) 

> > Dry incubation for improved lab hygiene 

> > Simple and intuitive operation due to predefined temperature 

keys (4 °C, 16 °C, 37 °C, 56 °C and 95 °C) 

> > Comprehensive range of program functions 

> > 2 second block exchange due to quick release system of 

SmartBlocks 

* 1 110 ° C can be set when using the 12 mm and cryo SmartBlocks. 




Power supply 


Interfaces 

Temperature accuracy 

20.6 × 30.4 × 13.1 cm / 8.1 x 12.0 x 5.2 in 

4.3 kg / 19.5 lb 

120 V, 50 – 60 Hz, 230 V, 50 – 60 Hz 

200 W 

USB interface 

±0.5 °C at 20-45 °C, 

±1.0 °C below 20 °C and above 45 °C 

Temperature setting range –10 °C to 100 °C 

Temperature control range From 30 °C 1) below RT to 110 °C 2) 

Timer 

15 sec to 99:30 h, unlimited 

1) 

±2 °C 2) 110° C when using the 12 mm and cryo SmartBlocks. 



Eppendorf ThermoStat C, basic device without thermoblock, 100 – 130 V/50 – 60 Hz 5383000027 $2,600.00 










5364000024 710.00 













34 35 



Eppendorf ThermoTop ® 


The ThermoTop is compatible with the ThermoMixer C, 

ThermoStat C, ThermoMixer F1.5 and ThermoMixer FP. 

Combined with the ThermoMixer C and ThermoStat C, the 

ThermoTop can be used with thermoblocks for plates and lab 

vessels with a volume up to 2 mL. Compatible thermoblocks 

feature the condens.protect symbol. 

When the ThermoTop is placed on the device, it begins heating 

immediately. The temperature optimally adapts to the test 

temperature to ensure that it does not negatively affect the 

sample. Condensation droplets have no opportunity to form. 





Description 

The new ThermoTop, with unique condens.protect technology, 

reliably prevents the formation of condensate on the tube 

lid and tube wall. Operation is simple, intuitive and does not 

require an additional cable connection. When the ThermoTop 

is placed on the device, it begins heating immediately. 

The temperature optimally adapts to the test temperature to 

ensure that it does not negatively affect the sample: optimal 

sample protection with optimal reaction conditions produce 

optimal results. 

Applications 

> > All temperature control procedures for which the formation 

of condensation on the tube lid and wall should be avoided 


> > Simple, wireless handling with automatic recognition, 

initialization and operation 

> > Efficient and reliable prevention of condensation on tube lid 

and wall 

> > Improves handling times, tubes don‘t have to be spun down at 

the end of the incubation 

> > ThermoTop improves temperature homogeneity in the 

reaction vessel 

> > Suitable for use with Thermomixer F1.5, Thermomixer FP 

as well as Thermomixer C and ThermoStat C with Eppendorf 

SmartBlocks 0.5 mL, 1.5 mL, 2.0 mL, plates, PCR 96 and PCR 

384 (see page 36 for more details) 

Advantages of condensation prevention during incubation 

Evaporation of H 2 

O and condensation on the tube lid changes 

the buffer concentration in the reaction. Enzymatic reactions 

can no longer run under optimal conditions. 

Use of the ThermoTop effectively prevents the formation of 

condensate, thus guaranteeing optimal reaction conditions. 

Vessels also no longer need to be spun down at the end of 

incubation which saves you valuable handling time. 




Eppendorf ThermoTop ® , with condens.protect technology 5308000003 $625.00 







36 37 



Eppendorf SmartBlocks TM 





Description Max. speed Max. temp. 

ThermoMixer C 

ThermoStat Lid 3) ThermoTop 

Eppendorf SmartBlock 0.5 mL 2,000 rpm 100 °C 




Eppendorf SmartBlock 15 mL 1,000 rpm 100 °C 

Eppendorf SmartBlock 50 mL 1,000 rpm 100 °C 

Eppendorf SmartBlock cryo 2,000 rpm 110 °C 2) 

Eppendorf SmartBlock 12 mm 2,000 rpm 110 °C 2) 

Eppendorf SmartBlock plates 3,000 rpm 1) 100 °C 4) 

Eppendorf SmartBlock PCR 96 2,000 rpm 100 °C 4) 

Eppendorf SmartBlock PCR 384 3,000 rpm 100 °C 4) 



SmartBlocks for the following reaction vessels: 

A. 24 x 1.5 mL vessels 

B. 24 x 0.5 mL vessels 

C. 24 x 2.0 mL vessels 

D. 8 x 5.0 mL vessels 

E. 8 x 15 mL conical tubes 

F. 4 x 50 mL conical tubes 

G. 24 x cryo tubes 

H. 24 lab vessels 11-11.9 mm in diameter 

I. 384-well PCR plates 

J. MTPs and deepwell plates 

K. 96-well PCR plates 

L. Lid for Eppendorf SmartBlocks plates, PCR 96 and 384 

Description 

Flexibility has never been this easy. Eppendorf offers a 

wide variety of exchangeable SmartBlocks for all common 

vessel formats from 5 µL to 50 mL for use with Eppendorf 

ThermoMixer C and ThermoStat C. All SmartBlocks are 

equipped with the unique Eppendorf QuickRelease system 

that makes the block exchange super fast and easy. Just press 

the lever on the front of the block and the block is exchanged 

in just seconds - no tools are needed. Screws and tools are no 

longer necessary. 


> > Fast and simple block exchange due to Eppendorf 

QuickRelease technology 

> > Optimized block design guarantees maximum temperature 

transfer to the sample 

> > Individually sensor-controlled Eppendorf SmartBlocks 

allow for block-specific calibration to provide maximum 

temperature accuracy and homogeneity 

> > Insulated thermoblocks for ergonomic operation - you won‘t 

burn your fingers 

> > Safe and ergonomic work via thermal insulation of blocks 

1) 

max. mixing frequency using DWP is 2,000 rpm 2) Only available with ThermoStat C 3) Maximum mixing frequency is 2,000 rpm when lid is used 4) Included with SmartBlock 



Eppendorf SmartBlock 0.5 mL, thermoblock for 24 reaction vessels 0.5 mL 5361000031 $710.00 








5364000024 710.00 












38 39 

Microcentrifuges 


Centrifuge 5427 R 





Description 

Centrifuge 5427 R was designed with high-throughput 

research applications in mind. With its aerosol-tight 48-place 

rotor it handles even the toughest challenges in a safe and 

reliable manner to provide you the efficiency boost you need. 

All aerosol-tight rotors, including the new rotors for 5.0 mL 

Eppendorf tubes as well as the 24 x 1.5/2 mL swing-bucket 

rotor, are equipped with the Eppendorf QuickLock lids for 

easy rotor lid opening and closing. 

Choose the Centrifuge 5427 R if a 24-place microcentrifuge 

(e.g., our Centrifuge 5424 R) doesn’t meet your needs 

regarding tube capacity and if you don’t need the extra 

versatility our cross-over Centrifuge 5430 R can offer you. 


> > Capacity: 48 x 1.5/2.0 mL, 12 x 5.0 mL 

> > Speed up to 25,000 x g (16,220 rpm) 

> > High-capacity 48-place rotor significantly increases 

productivity to save you valuable time 

> > Aerosol-tight Eppendorf QuickLock rotor lids allow for fast 

and ergonomic lid locking. 

> > Whisper quiet operation due to optimized sound design 

> > Soft-touch one-finger closure for ergonomic operation 

> > Temperature range from –10 °C to 40 °C 

> > FastTemp program optimized for fast 11 min pre-cooling 

> > ECO shut-off can be programmed to engage after 1, 2, 4 or 

8 hours of nonuse for maximum energy savings and extended 

compressor life 

> > Built-in condensation drain to eliminate water accumulation 


Max. RCF 

25,000 x g 

Max. speed 

16,220 rpm 

Max. capacity 

48 × 1.5/2.0 mL 

No. of rotors 9 

Acceleration time 

18 s 

Deceleration time 

18 s 

SOFT brake function 

Yes 

Timer 

10 s to 9:59 h 

Noise level 



40 41 

Centrifugation 


Rotors for Centrifuge 5427 R 

Rotor 48 x 1.5/2 mL, aerosol-tight (FA-45-48-11) 

Rotor 48 x 1.5/2 mL, aerosol-tight (F-45-48-11) 

> > Max. 18,210 x g (12,700 rpm) 

> > Rotor for 48 x 1.5/2.0 mL vessels 

> > Aerosol-tight with Eppendorf QuickLock ® lid 

> > Catalog No. 5409710001 

> > Max. 18,210 x g (12,700 rpm) 


> > Catalog No. 5409712004 



> > Max. 20,800 x g (14,000 rpm) 



> > Catalog No. 5409706004 

Rotor 30 x 1.5/2 mL (F-45-30-11) 

> > Max. 20,800 x g (14,000 rpm) 


> > Catalog No. 5409708007 




Rotor 24 x 1.5/2 mL, swing-bucket, aerosol-tight (S-24-11-AT) 



> > Max. 25,000 x g (16,220 rpm) 



> > Catalog No. 5409702009 

> > Max. 16,049 x g (12,700 rpm) 

> > Swing-bucket rotor for 24 x 1.5/2.0 mL vessels 


> > Catalog No. 5409715003 


Rotor 24 x spin column or 1.5/2 mL, aerosol-tight 

(FA-45-24-11-Kit) 

> > Max. 19,100 x g (13,200 rpm) 

> > Rotor with higher edge for safe centrifugation of up to 24 spin columns 


> > Catalog No. 5409704001 

Rotor 12 x 5.0 mL, aerosol-tight (FA-45-12-17) 

> > Max. 20,600 x g (14,000 rpm) 

> > Rotor for 12 x 5.0 mL Eppendorf Tubes 


> > Catalog No. 5409700006 







42 43 



Rotors for Centrifuge 5427 R 

Centrifuge 5430/5430 R 




Rotor 48 x 0.2 mL PCR tubes (F-45-48-5-PCR) 

> > Max. 11,710 x g (10,500 rpm) 

> > PCR strip rotor for up to 48 x 0.2 mL PCR tubes 

> > Catalog No. 5409714007 



Rotor 48 x 1.5/2.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-48-11) 5409710001 $1,070.00 

Rotor lid for FA-45-48-11, aerosol-tight, Eppendorf QuickLock ® 5409711008 330.00 

Rotor 48 x 1.5/2.0 mL with plastic lid (F-45-48-11) 5409712004 840.00 

Rotor lid for F-45-48-11 5409713000 194.00 

Rotor 30 x 1.5/2.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-30-11) 5409706004 970.00 



Rotor lid for F-45-30-11 5409709003 265.00 

Rotor 24 x 1.5/2.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-24-11) 5409702009 970.00 


Rotor 24 x 1.5/2.0 mL swing-bucket, with aerosol-tight QuickLock ® lid (S-24-11-AT) 5409715003 1,530.00 

Rotor lid for S-24-11-AT, aerosol-tight, Eppendorf QuickLock ® 5409720007 365.00 

Rotor 24 x 1.5/2.0 mL spin column kit, with aerosol-tight QuickLock ® lid (FA-45-24-11-Kit) 5409704001 1,070.00 

Rotor lid for FA-45-24-11-Kit, aerosol-tight, Eppendorf QuickLock ® 5409705008 259.00 

Rotor 12 x 5.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-12-17) 5409700006 970.00 


Adapter for 1.5/2.0 mL tubes, for use in 5.0 mL rotors, set of 4 pcs. 5820768002 28.70 

Adapter for cryo vials, for use in 5.0 mL rotors, set of 4 pcs. 5820769009 28.70 

Adapter for HPLC vials 12 x 32 mm, for use in 5.0 mL rotors, set of 4 pcs. 5820770007 28.70 

Rotor 6 x PCR-strips (F-45-48-5-PCR) 5409714007 755.00 

Rotors for Centrifuge 5430/5430 R 

Description 

Unique cross-over Centrifuges 5430 and 5430 R combine 

the best features of a microcentrifuge (small footprint) and 

multipurpose centrifuge (versatility) in one instrument. 

These centrifuges spin rotors for Eppendorf tubes and PCR 

strips as you would expect from any microcentrifuge. 

But that’s not all. In a compact size, Centrifuges 5430 and 

5430 R also accommodate a fixed-angle rotor for 15/50 mL 

conical tubes, Vacutainer, 10 to 50 mL OakRidge tubes, 

cryo and HPLC vials as well as a swing-bucket rotor for 

MTP and PCR plates. Until now, this has only been possible 

with large multipurpose centrifuges. 

Rotor FA-45-30-11 (update) 

> > Max. 20,817 x g (14,000 rpm) 


> > Aerosol-tight 

> > NEW: Eppendorf QuickLock ® lid 

> > Catalog No. 5427753001 




Rotor FA-45-24-11-Kit (update) 

> > Max. 19,090 x g (13,200 rpm) 

> > Rotor with higher edge for safe centrifugation of up to 24 spin columns 

> > Aerosol-tight 

> > NEW: Eppendorf QuickLock ® lid 

> > Catalog No. 5427752005 







44 45 



Rotors for Centrifuge 5430/5430 R 

Rotors 5430/5430 R 


Rotors 5430/5430 R 

Rotor F-45-48-11 (NEW) 

> > Max. 18,210 x g (12,700 rpm) 


> > Catalog No. 5427755004 

Rotor FA-45-16-17 (NEW) 

Rotor FA-45-48-11 (NEW) 

> > Max. 18,210 x g (12,700 rpm) 



> > Catalog No. 5427754008 

Rotor S-24-11-AT (NEW) 

> > Max. 16,049 x g (12,700 rpm) 

> > Swing-bucket rotor for 24 x 1.5/2.0 mL vessels 


> > Catalog No. 5427758003 



Rotor 30 x 1.5/2.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-30-11) 022654047 $ 970.00 


Rotor 24 x 1.5/2.0 mL spin column kit, with aerosol-tight QuickLock ® lid (FA-45-24-11-Kit) 022654128 1,070.00 

Rotor lid for FA-45-24-11-Kit, aerosol-tight, Eppendorf QuickLock ® 5427760008 259.00 

Rotor 48 x 1.5/2.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-48-11) 5427754008 1,070.00 



Rotor lid for F-45-48-11 5427756000 194.00 

Rotor 24 x 1.5/2.0 mL swing-bucket, with aerosol-tight QuickLock ® lid (S-24-11-AT) 5427757007 1,530.00 

Rotor lid for S-24-11-AT, aerosol-tight, Eppendorf QuickLock ® 5427758003 365.00 

Rotor 16 x 5.0 mL with aerosol-tight Eppendorf QuickLock ® lid (FA-45-16-17) 5427750002 1,070.00 


Adapter for 1.5/2.0 mL tubes, for use in 5.0 mL rotors, set of 4 pcs. 5820768002 28.70 

Adapter for cryo vials, for use in 5.0 mL rotors, set of 4 pcs. 5820769009 28.70 

Adapter for HPLC vials 12 x 32 mm, for use in 5.0 mL rotors, set of 4 pcs. 5820770007 28.70 

Adapters for rotor 6 x 15/50 mL (F-35-6-30) 

Adapter, for 1 tube 13 x 65 – 89 mm, small rotor bore, set of 2 pieces 5427740007 145.00 

Adapter, for 1 tube 13 x 65 – 89 mm, large rotor bore, set of 2 pieces 5427742000 145.00 

Adapter, for 1 tube 13 x 90 – 110 mm, small rotor bore, set of 2 pieces 5427741003 145.00 

Adapter, for 1 tube 13 x 90 – 110 mm, large rotor bore, set of 2 pieces 5427743006 145.00 

Adapter, for 1 Eppendorf Tube 5.0 mL, for small rotor bore, set of 2 pieces 5427746005 145.00 

Adapter, for 1 Eppendorf Tube 5.0 mL, for large rotor bore, set of 2 pieces 5427747001 145.00 




> > Max. 21,191 x g (14,200 rpm) 

> > Rotor for 16 Eppendorf Tubes 5.0 mL 


> > Catalog No. 5427750002 







46 47 



Centrifuge 5804/5804 R and 5810/5810 R 

Ordering information 5804 5804 R 

Model Standard Standard 20 A 

Description Catalog No. List USD Catalog No. List USD Catalog No. List USD 

Application-driven packages, (120 V; 50/60 Hz) 

Cell culture package (4 x 250 mL) 022628149 $7,350.00 022628153 $9,995.00 – – 

Cell culture package (4 x 100 mL) 022628135 6,750.00 022628146 9,500.00 – – 

High-Speed 15/50 mL package (6 x 50 mL conical) 022628211 7,550.00 – – – – 

Clinical package (4 x 250 mL) 022628048 7,350.00 022628051 9,995.00 – – 


Deepwell plate package (A-2-DWP) 022628203 6,590.00 022628225 10,150.00 – – 




Centrifuge 5804 Centrifuge 5804 R Centrifuge 5810 Centrifuge 5810 R 

Max. capacity 4 x 250 mL/2 x 5 MTP 4 x 750 mL/4 x 4 MTP 

Max. RCF 20,913 x g 20,913 x g 

Max. speed 14,000 rpm 14,000 rpm 

Rotors available 12 18 

Temperature range -9 °C to 40 °C -9 °C to 40 °C 

Dimensions (W × D × H) 47 × 55 × 34 cm / 

18 x 20 x 13 in 

Description 

Eppendorf Centrifuges 5804/5804 R and 5810/5810 R with 

their renowned quality and reliability offer you cost efficient 

solutions for your medium- to high-throughput applications - 

now and in the future. Centrifuge 5804/5804 R is a high 

speed centrifuge for (4 x 250 mL) medium capacity needs. 

It allows for molecular applications in tubes up to 100 mL 

and offers additional swing-bucket and fixed-angle rotors 

as well as deepwell plate capacity for increased versatility. 

Centrifuge 5810/5810 R is the most compact 3 liter centrifuge 

(4 x 750 mL) available on the market and a workhorse 

for medium to high-throughput laboratories. It combines 

extraordinary versatility and capacity for both tubes and 

plates with an extraordinary compact footprint. 


> > Swing-bucket rotors and adapters accommodate tubes and 

bottles from 0.2 mL to 750 mL 

> > Plate rotors for centrifugation of all types of MTP, PCR, cell 

culture, or deepwell plates 

> > Fixed-angle rotors for high-speed molecular biology 

applications in tubes from 0.2 mL to 100 mL 

> > Centrifugation speed of up to 20,913 x g (14,000 rpm) 

> > Aerosol-tight Eppendorf QuickLock ® caps and lids for easy, 

one-hand operation 

> > Centrifuge lid with soft-touch lid closure 

> > Low access height of 29 cm for easy loading and unloading of 

samples 

> > Quiet operation to improve your work environment 

> > Compact footprint saves valuable bench space 

> > Automatic rotor recognition and imbalance detection for 

maximum operational safety 

64 × 55 × 34 cm / 

25 x 20 x 13 in 

54 × 61 × 35 cm / 

21 x 24 x 13 in 

70 × 61 × 35 cm / 

28 x 24 x 13 in 

Weight w/o accessories 55 kg / 121 lb 80 kg / 176 lb 68 kg / 150 lb 99 kg / 218 lb 

Additional features of refrigerated Centrifuges 5804 R 

and 5810 R 

> > Temperature range from -9 °C to 40 °C 

> > FastTemp function for fast pre-cooling 

> > Standby cooling function holds temperature when centrifuge 

is not in use 

> > ECO shut-off engages after 8 hours of non-use to reduce 

energy consumption and to extend compressor life 

> > Dynamic compressor control (DCC) technology for optimized 

cooling performance 

Watch video clips of Centrifuge 5810 R incl. the new rotor 

generation on our dedicated website for mobile devices or at 

www.eppendorf.com/centrifugation 

Centrifuge options 

with 4 x 250 mL swing-bucket rotor (S-4-72), 120 V 5804000579 6,870.00 5805000572 9,760.00 – – 

with 4 x 100 mL swing-bucket rotor (A-4-44), 120 V 022629948 6,200.00 022629964 9,170.00 022629981 9,170.00 

without rotor, 120 V 022622501 5,015.00 022623508 8,540.00 022625080 8,540.00 

without rotor, 230 V 022622552 5,015.00 022623559 8,540.00 – – 

Ordering information 5810 5810 R 

Model Standard Standard 20 A 

Description Catalog No. List USD Catalog No. List USD Catalog No. List USD 

Application-driven packages, (120 V; 50/60 Hz) 

Cell culture package (4 x 750 mL) 022628185 $9,950.00 022628187 $12,870.00 022628188 $12,870.00 

Cell culture package (4 x 500 mL) 022628179 9,400.00 022628180 12,450.00 022628181 12,450.00 

Cell culture package (4 x 250 mL) 022628157 8,590.00 022628168 11,600.00 022628169 11,600.00 

High-Speed 15/50mL package (6 x 50 mL conical) – – 022628257 12,450.00 022628258 12,450.00 




Centrifuge options 

with 4 x 750 mL swing-bucket rotor (S-4-104), 120 V 022627110 9,480.00 022627122 12,500.00 022627133 12,500.00 



without rotor, 120 V 022625004 6,460.00 022625501 9,990.00 022625101 9,990.00 

without rotor, 230 V 022625055 6,460.00 022625551 9,990.00 – – 

Cell Culture package includes a centrifuge and swing-bucket rotor plus the following adapter (sets of 2) for 15 mL and 50 mL conical tubes: 5804/R with 4 x 100 mL rotor = 2 x 022637606, 2 x 022637665; 

5804/R with 4 x 250 mL rotor = 2 x 5804783000, 2 x 5804784006; 5810/R with 4 x 250 mL rotor = 2 x 022638289, 2 x 022638351; 5810/R with 4 x 500 mL rotor = 2 x 022638742, 2 x 022638769; 

5810/R with 4 x 750 mL rotor = 2 x 5825734009, 2 x 5825733002. 

High-Speed 15/50 mL package includes the listed centrifuge and 6 x 50 mL conical fixed-angle rotor FA-45-6-30 plus 7 adapters for 15 mL conical tubes (1 x 5820718005) 

Clinical package includes a centrifuge and swing-bucket rotor plus following adapter (sets of 2) for 13 mm and 16 mm tubes: 5804/R with 4 x 100 mL = 2 x 022637541, 2 x 022637568; 

5804/R with 4 x 250 mL = 2 x 5804789008, 2 x 5804791002; 5810/R with 4 x 250 mL rotor = 2 x 022638246, 2 x 022638262; 5810/R with 4 x 500 mL rotor = 2 x 022638718, 2 x 022638721; 

5810/R with 4 x 750 mL rotor = 2 x 5825738004, 2 x 5825736001. 

Plate package includes the listed centrifuge and deepwell plate rotor A-2-DWP. Maximum loading height of A-2-DWP is 89 mm per bucket. 










48 49 



Overview of the main rotors 

Rotor S-4-104 Rotor A-4-81 Rotor A-4-62 Rotor S-4-72 Rotor A-4-44 Rotor A-2-DWP-AT Rotor A-2-DWP Rotor F-34-6-38 Rotor FA-45-6-30 Rotor FA-45-20-17 Rotor FA-45-48-11 



Max. capacity 4 x 750 mL, 

aerosol-tight, 

Eppendorf 

QuickLock ® 

4 x 500 mL, 

aerosol-tight 

4 x 250 mL, 

aerosol-tight 

4 x 250 mL 4 x 100 mL, 

aerosol-tight 

2 x 6 MTP, 

aerosol-tight 

2 x 5 MTP 6 x 85 mL 6 x 50 mL, 



QuickLock ® 

20 x 5 mL 



QuickLock ® 

48 x 2 mL, 



QuickLock ® 

5804/5804 R 

5810/5810 R 

Description High-capacity 

swing-bucket 

rotor for blood, 

bacteria, cell 

culture and plate 

centrifugation 

Medium to 

high capacity 

swing-bucket 




Economic 

swing-bucket 





Economic swingbucket 

rotor for 

blood, bacteria, 

cell culture 


High-speed 

swing-bucket 

rotor for platelet 

poor plasma, 

blood, bacteria 

and cell culture 

Aerosol-tight 

deepwell plate 

rotor for safe 


of hazardous 

samples. 

Economic 

deepwell plate 

rotor 

High-speed 

rotor suitable 

for molecular 

applications 

in tubes up to 

85 mL 

Aerosol-tight 

high-speed 

rotor for safe 


of hazardous 

samples 

Aerosol-tight 

high-speed rotor 

for Eppendorf 

5 mL tubes 

Aerosol-tight 

microcentrifuge 

tube rotor for safe 


of hazardous 

samples 



Aerosol-tightness 

Max. RCF 3,214 x g 3,220 x g 3,220 x g 3,234 x g 4,500 x g 3,486 x g 2,250 x g 18,500/15,500 × g 1) 20,130/16,639 × g 1) 20,913 x g 19,083 x g 

Max. speed 3,900 rpm 4,000 rpm 4,000 rpm 4,200 rpm 5,000 rpm 4,500 rpm 3,700 rpm 12,000/11,000 

rpm 

Volume range 

0.2 – 750 mL, 

MTP/DWP 

0.2 – 500 mL, 

MTP/DWP 

1) 

Max. speed in Centrifuge 5810 R, lower speed applies to Centrifuge 5804/5804R/5810. 

0.2 – 250 mL, 

MTP/DWP 

12,100/11,000 

rpm 

13,100 rpm 13,000 rpm 

0.2 – 250 mL 0.2 – 100 mL MTP/DWP MTP/DWP 0.2 – 85 mL 5 – 50 mL 0.2 – 5.0 mL 0.2 – 2.0 mL 




Advanced rotor design – swing-bucket rotors 

Advanced rotor design – fixed-angle rotors 


Description 

Improved energy efficiency 

The new round buckets have been designed to be especially 

aerodynamic. The result is a more efficient design that reduces 

power consumption by up to 48 %. 

Longer rotor lifetime 

The new rotor generation of both swing-bucket and fixed-angle 

rotors has been designed with a longer lifetime in mind. 

Eppendorf QuickLock ® lids for ergonomic lid locking 

Our aerosol-tight QuickLock rotors close with only ¼ of a turn. 

This not only saves you time during every day use, it also takes 

repetitive stress off your wrist. 

Superior temperature control 

Aluminium, with its high heat conductivity, allows fast precooling 

and accurate temperature control in refrigerated 

centrifuges. 


> > New Eppendorf QuickLock ® caps for one-hand operation 

> > Significantly improved handling, easy operation with one or 

two hands 

> > Handle allows the closed bucket to be carried to the biosafety 

cabinet where it can be opened in a safe environment. 

> > Aerosol-tightness has been tested and verified by HPA, Porton 

Down, UK 

Description 

Low rotor weight 

Eppendorf’s fixed-angle rotors are among the lightest in the 

industry to improve handling and operational safety. Lightweight 

rotors cause less wear and tear on the motor and allow 

for faster acceleration and braking times. 

Additional product features 

> > All rotors are anodized for high chemical resistance and to 

prevent corrosion 

> > Optimized design of rotor bore holes and adapters provide 

optimal support for high-speed centrifugation of tubes 

> > Aluminium rotors are recyclable 







50 51 





Rotor 4 x 750 mL (S-4-104) 

Rotor S-4-104 

> > High capacity swing-bucket rotor with max. speed 3,214 × g 

(3,900 rpm) * 1 

> > Broad range of adapters enables high versatility for tubes and flasks 

from 0.2 mL to 750 mL and plates 

> > Aerodynamic round bucket design for up to 36 % energy savings 

compared to Rotor A-4-81 

> > Mixed loading with 2 plate buckets and 2 round tube buckets allows for 

increased versatility 

> > Aerosol-tight QuickLock caps for safe centrifugation of hazardous 

samples optionally available * 2 

> > Rotor lifetime: max. 100,000 cycles 

> > Buckets, lids and adapters are autoclavable 

*1 Software upgrade package for 120 V centrifuges with serial numbers between: 5810 = 12,949 – 62,206, 

5810 R = 37,842 – 66,386. Package includes rotor 5820740000, an on-site software upgrade for one 

58xx centrifuge by an Eppendorf service technician plus free preventive maintenance inspection. 

Price includes software, travel and labor. 

*2 Aerosol-tightness tested by Health Protection Agency, Porton Down, UK. 


Description Max. speed Max. RCF Max. radius Catalog No. List USD 

Rotor S-4-104 options 

Rotor S-4-104, incl. 4 × 750 mL round buckets 3,900 rpm 3,214 x g 189 mm 5820740000 $3,510.00 

Rotor S-4-104, incl. 4 x plate buckets (AT-capable) 3,900 rpm 2,568 x g 151 mm 5820754001 3,510.00 

Rotor S-4-104, incl. 4 x plate buckets 3,900 rpm 2,619 x g 154 mm 5820753005 3,510.00 

Rotor S-4-104, with software upgrade package 3 3,900 rpm 3,214 x g 189 mm 022639905 4,295.00 

Rotor S-4-104, without buckets - - 5820755008 1,900.00 

Buckets for Rotor S-4-104 

Bucket, 750 mL round, set of 4 3,900 rpm 3,214 x g 189 mm 5820741007 1,765.00 

Bucket, 750 mL round, set of 2 3,900 rpm 3,214 x g 189 mm 5820742003 917.00 

Plate bucket (AT-capable), incl. plate carrier, set of 4 3,900 rpm 2,568 x g 154 mm 022638935 

Plate bucket (AT-capable), incl. plate carrier, set of 2 3,900 rpm 2,568 x g 151 mm 022638930 917.00 

Plate bucket, set of 4 3,900 rpm 2,619 x g 154 mm 022638945 1,765.00 

Plate bucket, set of 2 3,900 rpm 2,619 x g 154 mm 022638940 917.00 

Bucket for microfluidic card, set of 2 3,900 rpm - 5820751002 Inquire 

Accessories 

Description Max. speed Max. RCF Max. radius Catalog No. List USD 

Aerosol-tight cap, for 750 mL round buckets, 

- 5820747005 $250.00 

Eppendorf QuickLock ® , set of 2 pieces 

Aerosol-tight cap, for plate buckets (AT-capable), 

- 022638937 281.00 

Eppendorf QuickLock ® , set of 2 pieces 

Bottle, 750 mL, wide-neck, set of 2 pieces 3900 rpm 3146 × g 185 mm 5820708000 270.00 




Tube/plate 

tubes 

1.5/2.0 mL 

roundbottom 

tubes 

2.6 – 8 mL 

roundbottom 

tubes 

5.5 – 12 mL 

roundbottom 

tubes 

9 mL 


Tubes 5.0 mL 

conical tubes 

15 mL 

conical tubes 

50 mL 

conical tubes, 

skirted 50 mL 

bottle 

Corning ® 

250 mL flat, 500 mL 

175 – 225 mL 

conical 2) 

Max. tube diameter 11 mm 13 mm 16 mm 17.5 mm 17 mm 17 mm 31 mm 31 mm 62 mm 98 mm 104 mm – – 

Max. vessel height 39 mm 115 mm 119 mm 111 mm 60 mm 125 mm 122 mm 120 mm 145 mm 136 mm 150 mm 60 mm 60 mm 

Max. vessel height for 39 mm 108 mm 114 mm 106 mm 60 mm 120 mm 116 mm 116 mm 125 mm – – – 45 mm 

cap/lid 1) 

use with aerosol-tight 

Adapter 


bottle 

750 mL 

MTP, DWP, 

PCR plates 

MTP, DWP, 

PCR plates 


Number of vessels 

per adapter/rotor 

Adapter bottom 

shape 

50/200 23/92 20/80 20/80 14/56 14/56 7/28 5/20 1/4 1/4 1/4 4/16 (MTP) 

1/4 (DWP) 

flat round round round conical conical conical flat flat conical flat flat flat 

Centrifugation radius 188 mm 179 mm 180 mm 179 mm 189 mm 188 mm 187 mm 178 mm 183 mm 187 mm 185 mm 154 mm 151 mm 

Max. RCF 3,197 x g 3,044 x g 3,061 x g 3,044 x g 3,214 x g 3,197 x g 3,180 x g 3,027 x g 3,112 x g 3,146 x g 3,146 x g 2,619 x g 2,568 x g 

Packing set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. 

Catalog No. 5825740009 5825738004 5825736001 5825743008 5825739000 5825734009 5825733002 5825732006 5825741005 5825745000 5825744004 022638940 022638930 

List USD $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $290.00 $917.00 $917.00 

1) 

Exceeding the maximum tube length may reduce the loading capacity of the rotor. Please perform a manual swing-out test before starting the centrifuge run to make sure the tubes do not hit 

the rotor yoke. 2) Please note that these conical bottles need to be used together with a conical form insert provided by the manufacturer. 

4/16 (MTP) 

1/4 (DWP) 

3) 

Software upgrade package for 120 V centrifuges with serial numbers between: 5810 = 12,949 – 62,206, 5810 R = 37,842 – 66,386. Package includes rotor 5820740000, an on-site 

software upgrade for one 58xx centrifuge by an Eppendorf service technician plus free preventive maintenance inspection. Price includes software, travel and labor. 







52 53 



Rotor 4 x 250 mL (S-4-72) 


Rotor S-4-72 

> > Medium capacity swing-bucket rotor with speed up to 3,234 × g 

(4,200 rpm) 

> > Broad range of adapters available to handle all common tube formats 

from 0.2 mL to 250 mL 

> > Aerodynamic round bucket design for up to 48 % energy savings 

compared to Rotor A-4-62 

> > Rotor life time: max. 60,000 cycles 

> > Buckets and adapters are autoclavable 


Description Max. speed Max. RCF Catalog No. List USD 

Rotor S-4-72, incl. 4 × 250 mL round buckets 4,200 rpm 3,234 x g 5804746007 $2,195.00 

Rotor S-4-72, with software upgrade package 3 4,200 rpm 3,234 x g 022639907 2,975.00 

Round bucket 250 mL, for Rotor S-4-72, 

Set of 4 pcs. 

4,200 rpm 3,234 x g 5804747003 1,445.00 





Tube/plate tubes 1.5/2.0 mL round-bottom 

tubes 2.6 – 8 mL 

round-bottom 

tubes 5.5 – 10 mL 

round-bottom 

tubes 9 mL 

Eppendorf Tubes 

5.0 mL 

conical tubes 15 mL conical tubes 50 mL conical tubes, skirted 

50 mL 

Max. tube diameter 11 mm 13 mm 16 mm 17.5 mm 17 mm 17 mm 31 mm 31 mm 62 mm 

Max. vessel height 43 mm 115 mm 112 mm 113 mm 60 mm 120 mm 120 mm 120 mm 130 mm 

Max. vessel height for use with – – – – – – – – – 

aerosol-tight cap/lid 1) 

Adapter 

bottle 250 mL flat, 

175 – 225 mL conical 2) 


Number of vessels per adapter/ 26/104 14/56 13/52 12/48 8/32 8/32 4/16 2/8 1/4 

rotor 

Adapter bottom shape flat round round round conical conical conical flat flat 

Centrifugation radius 159 mm 159 mm 157 mm 158 mm 163 mm 164 mm 164 mm 153 mm 160 mm 

Max. RCF 3,136 x g 3,136 x g 3,096 x g 3,116 x g 3,215 x g 3,234 x g 3,234 x g 3,017 x g 3,175 x g 

Packing set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. set of 2 pcs. 

Catalog No. 5804794001 5804789008 5804791002 5804792009 5804793005 5804783000 5804784006 5804785002 5804787005 

List USD $270.00 $270.00 $270.00 $270.00 $270.00 $270.00 $270.00 $270.00 $270.00 

1) 

Exceeding the maximum tube length may reduce the loading capacity of the rotor. Please perform a manual swing-out test before starting the centrifuge run to make sure the tubes do not 

hit the rotor yoke. 2) Please note that these conical bottles need to be used together with a conical form insert provided by the manufacturer. 3) Software upgrade package for 120 V centrifuges 

with serial numbers between: 5804 = 13,031 – 62,247, 5804R = 33,350 – 63,063, 5810 = 12,949 – 62,206, 5810R = 37,842 – 66,386. Package includes rotor 5804746007, an on-site software 

upgrade for one 58xx centrifuge by an Eppendorf service technician plus free preventive maintenance inspection. Price includes software, travel and labor. 







54 55 



Rotor F-35-48-17* 1 , Rotor FA-45-20-17* 1 

and Rotor FA-45-48-11* 1 

Rolling cabinet 

Rotor 48 x 15 mL (F-35-48-17) 




Rotor 20 x 5 mL (FA-45-20-17) 

> > 20 places for Eppendorf Tube 5.0 mL 

> > Max. speed: 20,913 × g (13,100 rpm) 

> > Aerosol-tight Eppendorf QuickLock ® lid 

> > Rotor lifetime: no lifetime limitation 

> > Catalog No. 5820765003 

> > High-capacity fixed-angle rotor for up to 40 × 15 mL conical tubes or 

48 × 15 mL glass tubes 

> > Maximum speed: 5,050 × g (5,500 rpm) 

> > Form inserts for 15 mL conical tubes and rubber mats for glass tubes 

included 

> > Rotor lifetime: 75,000 cycles 

> > Catalog No. 5820772000 

Rotor 48 x 1.5/2 mL (FA-45-48-11) 

> > 48 places for 1.5/2.0 mL tubes 

> > Max. speed: 19,083 × g (max. 13,000 rpm) 

> > Aerosol-tight Eppendorf QuickLock ® lid 

> > Rotor lifetime: 75,000 cycles 

> > Catalog No. 5820760001 

*1 

Please note that a software upgrade will be necessary for older centrifuges when using this rotor. Please contact your local Eppendorf organization or dealer for further information. 

Description 

If space is a limited resource in your lab, Eppendorf’s rolling 

cabinet can give you the freedom and flexibility to use your 

Centrifuge 5804/5804 R or 5810/5810 R wherever you need 

it. Use the rolling cabinet if there is not enough space on the 

regular lab bench or to transport and use the centrifuge in 

other labs. The included drawer provides extra storage space 

for your rotors and adapters. 


> > Rolling cabinet with closed centrifuge fits under standard 

90 cm lab benches 

> > With 4 casters (2 lockable) and drawer 

> > Dimensions (W × D × H): 70 × 45 × 62 cm 






Rotor F-35-48-17, incl. 48 steel sleeves and adapters 5820772000 $ 984.00 

Rotor F-35-48-17, incl. 24 steel sleeves and adapters 5820771003 666.00 

Rotor FA-45-20-17, for 20 Eppendorf Tubes 5.0 mL, aerosol-tight, Eppendorf QuickLock ® 5820765003 1,670.00 

Adapter, for 1 Vessel 1.5 – 2.0 mL, for rotors FA-45-12-17, FA-45-16-17 and FA-45-20-17, 5820768002 28.70 

Set of 4 pcs. 

Adapter, for 1 Cryo tube, for rotors FA-45-12-17, FA-45-16-17 and FA-45-20-17, 

5820769009 28.70 

Set of 4 pcs. 

Adapter, for 1 tube 5 mL, for rotors FA-45-12-17, FA-45-16-17 and FA-45-20-17, 

5820770007 28.70 

Set of 4 pcs. 

Rotor FA-45-48-11, for 48 × 1.5/2.0 mL tubes, aerosol-tight, Eppendorf QuickLock ® 5820760001 1,670.00 



Rolling cabinet, for Centrifuge 5804/5804 R and 5810/5810 R 5821020010 $2,210.00 







56 57 

Service 

Service 

Rotor Assurance Plans 

epServices 

for premium performance 

Service 

Service 

Service 

Rotors and buckets are subject to normal wear and tear during 

regular operation and may be damaged as a result. Surface 

scratches, among others, can affect the chemical resistance 

and accelerate the corrosion process. The stress of high 

number of cycles increases the likelihood of microscopic 

cracks. These microcracks can be detected early on during 

a qualified inspection, which will allow the rotor to be 

exchanged before serious damage has occurred. 

Our certified, qualified service technicians use sophisticated 

borescope devices to detect invisible, yet critical, cracks and 

scratches. If none or very few surface changes are identified, 

the rotor can be used according to instructions - or for an 

additional year if the inspection was performed at the end of 

the recommended service life. 

Rotor Assurance Plans are services performed in your 

laboratory by a qualified Eppendorf Field Service Engineer. 

This service can be delivered in conjunction with other 

services such as a preventive maintenance (PM) or separately 

as an independent rotor check. This service is provided at 

prevailing time and travel rates and rotors need to be clean 

to achieve a proper reading and interpretation. 

Inspection service for rotors 

Recommended service periods 

The Eppendorf Assurance Plans feature 

> > Confidence for safest operation 

> > Rotor care/use instructions 

> > Detailed test report 

> > Discount on new rotor 

Advantages of the inspection service 

> > Depend on safe operation 

> > Sample integrity and consistent performance 

> > Maximizing rotor service life 

i More information available at: www.eppendorf.com/epServices. Not available in all countries. 

Group 1 

Fixed-angle rotors 

F-45-12-11, F-45-24-11, 

F-45-30-11, FA-45-18-11, 

FA-45-24-11 series, 

FA-45-30-11, F-45-18-17-Cryo 

Group 2 

Fixed-angle rotors 

Visual rotor check* Annually 5424860109 5424860109 5810860113 

Advanced rotor check From twice per service life to annually 5424860206 5810860237 5810860245 

F-35-6-30, 

F-34-6-38, 

FA-45-6-30 

Group 3 

Swing-bucket rotors & buckets 

A-2-MTP, A-2-DWP, A-4-44, A-4-62, 

A-4-81 

Service 

Service 

Service 

*For all Eppendorf rotors and accessories 







58 59 

PCR 

PCR 

Mastercycler ® nexus 

Mastercycler ® nexus GSX1 Mastercycler ® nexus SX1 Mastercycler ® nexus GSX1e Mastercycler ® nexus SX1e Mastercycler ® nexus flat Mastercycler ® nexus flat eco 

PCR 

PCR 

PCR 

Intuitive graphic programming ■ ■ 

E-mail Notification ■ ■ ■ ■ ■ ■ 

Flexlid TM concept ■ ■ ■ ■ ■ ■ 

2 years warranty ■ ■ ■ ■ ■ ■ 

Universal Block for 0.2 mL and 0.5 mL vessels 

Silver block (5 °C/s heating) ■ ■ ■ 

Flat block for in-situ PCR 

Gradient block ■ ■ ■ ■ 

Unit can control 2 other units in network ■ ■ 

Unit can only be controlled 1) ■ ■ ■ ■ 

USB port ■ ■ 

Block homogeneity 20–72 °C ≤ ± 0.3 °C ≤ ± 0.3 °C ≤ ± 0.3 °C ≤ ± 0.3 °C ≤ ± 0.3 °C ≤ ± 0.3 °C 

Block homogeneity 90 °C ≤ ± 0.4 °C ≤ ± 0.4 °C ≤ ± 0.4 °C ≤ ± 0.4 °C ≤ ± 0.4 °C ≤ ± 0.4 °C 

Block temperature accuracy ± 0.2 °C ± 0.2 °C ± 0.2 °C ± 0.2 °C ± 0.2 °C ± 0.2 °C 

Gradient block Over 12 series (0.2 mL) Over 12 series (0.2 mL) 

Spread of min. gradient 0.1 °C 0.1 °C 

Heating rate 5 °C/s 5 °C/s 5 °C/s 5 °C/s 3 °C/s 3 °C/s 

Heating technology of the block 

Interfaces 

Peltier elements, triple 

circuit technology 

CAN in, CAN out, USB, 

Ethernet 



CAN in, CAN out, USB, 

Ethernet 

Peltier elements, triple circuit 

technology 





CAN in, CAN out, Ethernet CAN in, CAN out, Ethernet CAN in, CAN out, USB, 

Ethernet 



CAN in, CAN out, Ethernet 

Lid descent and closing pressure flexlid technology with TSP flexlid technology with TSP flexlid technology with TSP flexlid technology with TSP flexlid technology with TSP flexlid technology with TSP 

Lid temperature range 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 

Number of programs on device > 700 > 700 > 700 > 700 > 700 > 700 

Sample capacity 96 x 0.2 mL PCR vessels, 1 

PCR plate 8 x 12 or up to 71 

x 0.5 mL PCR vessels 

96 x 0.2 mL PCR vessels, 1 




PCR plate 8 x 12 or up to 71 x 

0.5 mL PCR vessels 




4 slides or other matching 

consumables 

Temperature control range of the block 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 

Gradient temperature range 30 – 99 °C – 30 – 99 °C – – – 

Temperature control mode 

Fast, standard, safe, also 

available in gradient 

operation 

Fast, standard, safe 

Fast, standard, safe, also 

available in gradient 

operation 

4 slides or other matching 

consumables 

Fast, standard, safe Fast, standard, safe Fast, standard, safe 

Thermoblock Silver Silver Silver Silver Aluminum Aluminum 

Max. power consumption 540 W 540 W 540 W 540 W 700 W 700 W 

Dimensions (W × D × H) 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 

Total weight 10.9 kg 10.9 kg 10.4 kg 10.4 kg 10.4 kg 10.4 kg 

1) 

Control through a Mastercycler ® nexus, Mastercycler ® nexus gradient, Mastercycler ® nexus X1g, Mastercycler ® nexus X1 or Mastercycler ® nexus flat 

PCR 

PCR 

PCR 







60 61 

PCR 

PCR 

Mastercycler ® nexus X1 

Mastercycler ® nexus flat and Mastercycler ® flat eco 

PCR 

PCR 

PCR 

PCR 

PCR 

Description 

In the age of networking, the new Mastercycler nexus is your 

reliable companion when it comes to the daily routine of PCR. 

It is easy to use, does not need much space or energy and 

sends you an e-mail when it is done – what else do you need 

from a PCR cycler 

Combine, Connect, Control 

> > Combine up to 3 units for ultimate throughput 

> > Connect your Mastercycler nexus to your computer network 

and get a status e-mail to your desk 

> > Control all the relevant parameters of your PCR through the 

intuitive software 


> > Intuitive graphic programming 

> > Small footprint 

> > Connect up to 2 Mastercycler nexus eco to your existing unit 

> > E-mail notification 

> > flexlid concept allows use of all types of consumables with 

automatic height adjustment of the lid 

> > Heating rate: 5 °C/s 

> > Fast silver block 

Description 

Special applications require special consumables. With its 

flat block and no wells, the Mastercycler nexus flat offers the 

optimal foundation for slides and other unusual consumable 

formats. 

Combine, Connect, Control 

> > Combine up to 3 units for ultimate throughput! 

> > Connect your Mastercycler nexus to your computer network 

and get a status e-mail to your desk 

> > Control all the relevant parameters of your PCR through the 

intuitive software 


> > Intuitive graphic programming 

> > Small footprint 

> > Connect up to 2 Mastercycler nexus eco to your existing unit 

> > E-mail notification 

PCR 

Applications 

> > Standard PCR 

> > Cycle sequencing 

> > In situ PCR with adapter 



Mastercycler ® nexus SX1, 110 V/50 – 60 Hz 6346000021 $7,900.00 

Mastercycler ® nexus GSX1, 110 V/50 – 60 Hz 6345000028 9,250.00 

Mastercycler ® nexus SX1e, 110 V/50 – 60 Hz 6348000029 6,300.00 

Mastercycler ® nexus GSX1e, 100 V/50 – 60 Hz 6347000025 7,590.00 

CAN bus connecting cable, 150 cm 950014016 53.00 


Self-test dongle 950030040 316.00 



Mastercycler ® nexus flat, 110 V/50 – 60 Hz 6335000020 $7,400.00 

Mastercycler ® nexus flat eco, 110 V/50 – 60 Hz 6330000021 6,500.00 



Self-test dongle 950030040 316.00 







62 63 

Photometry 

Photometry 

Eppendorf BioSpectrometer ® fluorescence 


Mode Spectrophotometer Fluorometer 

Photometry 

Photometry 

Photometry 

Description 

The BioSpectrometer fluorescence has all of the features 

of the BioSpectrometer basic, plus the option to determine 

very low concentrations of biomolecules using fluorescent 

dyes. In the absorption range, spectra measurements can be 

made between 200 nm to 830 nm. The fluorescence unit of 

the BioSpectrometer fluorescence can be used to increase 

the measuring range by a factor of 1,000, for detecting DNA 

for example. This means that DNA can be reliably quantified 

well beyond the common lower photometric detection limit of 

approx. 2.5 ng/µL, and up to a concentration of 2.5 pg/µL. 

The fluorescence intensity is determined using an excitation 

wavelength of 470 nm and emission wavelengths of 520 nm 

and 560 nm. Well-known dyes for nucleic acid and protein 

quantification, such as PicoGreen, OliGreen, RiboGreen and 

NanoOrange, can be measured. 

Applications 

> > Absorbance measurement for one or more wavelengths, 

recording of wavelength scans 

> > Applications with evaluations via factor, standard or 

standard series 

> > Two-wavelength method with subtraction and division 

evaluation 

> > Concentration via standard series (including linear and nonlinear 

regression and linear interpolation) 

> > Sensitive nucleic acid and protein quantification via 

fluorescence intensity 


> > All options of the BioSpectrometer basic 

> > UV/VIS spectral range 200 nm to 830 nm 

> > Preprogrammed and freely selectable applications and 

fluorescence applications 

> > Data transfer via USB interface 

> > Fluorescence intensity across a range of 0.5 nM to 2,000 nM 

fluorescein 

> > Fluorescence excitation wavelength of 470 nm 

> > Fluorescence emission wavelengths at 520 nm and 560 nm 

> > Compatible with microliter measuring cells and standard 

cuvettes 

Measuring principle 

Single-beam absorption 

spectrophotometer with reference beam 

Confocal filter fluorometer with 

reference beam 

Light source Xenon flash lamp LED 

Excitation wavelength 

470 nm, bandwidth: 25 nm 

Emission wavelength I 


Emission wavelength II 


Wavelength range 

200 nm – 830 nm 

Smallest step size 

1 nm 

Spectral bandwidth 

≤ 4 nm 

Absorption measuring range 0 A – 3,0 A (260 nm) 0.5 nM – 2 000 nM fluorescein 

(emission wavelength 520 nm) 

Random error ≤ 0.002 at A = 0 

≤ 0.005 (0.5 %) at A = 1 

± 2 % at 1 nM fluorescein (emission 

wavelength 520 nm) 

Systematic error ± 1 % (A = 1) 

Light path height 8.5 mm 8.5 mm 

Cuvette shaft 12.5 mm × 12.5 mm, untempered 12.5 mm × 12.5 mm, untempered 

Beam receiver CMOS photodiode array Photodiodes 

Interfaces 

USB master: for USB stick 

USB slave: for connecting to a PC 

Interface for Eppendorf thermal printer: serial RS232 

Power consumption 

Maximum expected power per name plate: 25 W 

Approx. 15 W during operating step 

Approx. 5 W with dimmed display 

Power supply 

100 – 240 V, 50 – 60 Hz 


295 × 400 × 150 mm 


5.4 kg 

Display 

5.7“ VGA TFT display 

Operator guidance language 

Spanish, Italian, French, English, German 

Cuvette material 

For measurements in the UV range: quartz glass or UV-transparent plastic 

(Eppendorf UVette ® , 220 nm to 1,600 nm) 

For measurements in the visible range: glass or plastic 

For microvolume measurements: microvolume measuring cells such as the 

Eppendorf µCuvette G1.0 



Eppendorf BioSpectrometer ® fluorescence, 110 V/50 – 60 Hz 6137000014 $11,810.00 

BioSpectrometer fluorescence reference filter set, filter set for verifying photometric 

accuracy and wavelength systematic error (according to NIST) and verifying fluorimetric 

precision (random error) and linearity 

6137928009 3,752.00 

Photometry 

Photometry 

Photometry 







64 65 

Photometry 

Photometry 

Eppendorf BioPhotometer ® D30 

Photometry 

Photometry 

Photometry 

Photometry 

Description 

The Eppendorf BioPhotometer D30 is the 3rd generation of 

Eppendorf BioPhotometers, which have become an established 

standard in the field of life sciences. The measurement 

data will be recorded for fixed wavelengths, making the 

BioPhotometer D30 the ideal device for routine applications. 

Data is clearly processed making the evaluation of results fast 

and simple while minimizing the risk of mistakes. Additional 

assistance for the user, including comparative spectra that 

are stored in the device, offer an additional level of security. 

Furthermore, all relevant ratios are automatically determined 

for the corresponding applications. 

The BioPhotometer D30 offers the option of recording purity 

scans for specific applications. In a defined measuring 

range, additional measurement data is collected and shown 

extrapolated, allowing contamination in the sample to be 

visually identified quickly. 

Applications 

> > Absorption measurements for one or more fixed wavelengths 

> > Applications with evaluations via factor, standard or standard 

series 


> > Fixed wavelengths at 230 nm, 260 nm, 280 nm, 320 nm, 

340 nm, 405 nm, 490 nm, 562 nm, 595 nm and 600 nm 

> > Display of purity scan for specific applications 

> > Preprogrammed applications for quick start 

> > Integrated application and results memory 

> > Data transfer via USB interface 

> > Direct printer port available 

> > Xenon flash lamp with very long life time 

> > Guided software process to minimize errors 

> > Help box with explanation of each individual step in 

5 languages 

> > Compatible with microvolume measuring cells and 

standard cuvettes 


Dimensions (W × D × H) 295 × 400 × 150 mm 

Weight w/o accessories 5.4 kg 

Power supply 

100 – 240 V, 50 – 60 Hz 

Interfaces 

USB master: for USB stick 

USB slave: for connecting to a PC 

Interface for Eppendorf thermal printer: serial RS232 

Display 

5.7“ VGA TFT display 

Beam receiver 

CMOS photodiodes 

Cuvette material 

For measurements in the UV range: quartz glass or UV-transparent plastic (Eppendorf 

UVette ® , 220 nm to 1,600 nm) 

For measurements in the visible range: glass or plastic 

For microvolume measurements: microvolume measuring cells such as the Eppendorf 

µCuvette G1.0 

Cuvette shaft 

12.5 mm × 12.5 mm, untempered 

Light path height 

8.5 mm 

Light source 

Xenon flash lamp 

Absorption measuring range 0 A – 3,0 A (260 nm) 

Operator guidance language Spanish, Italian, French, English, German 

Random error 

± 0.5 nm 

Spectral bandwidth 

≤ 4 nm 

Systematic error 

± 1 nm 

Wavelengths (nm) 230, 260, 280, 320, 340, 405, 490, 562, 595, 600 



Eppendorf BioPhotometer ® D30, 110 V/50 – 60 Hz 6133000010 $6,240.00 

Eppendorf µCuvette G1.0 & BioPhotometer D30 (bundle), Eppendorf microvolume 6133000940 7,753.00 

measuring cell and BioPhotometer D30, 110 V/50 – 60 Hz 

BioPhotometer D30 reference filter set, filter set for verifying photometric accuracy and 

wavelength systematic error (according to NIST) 

6133928004 1,750.00 

Photometry 

Photometry 







66 67 

Photometry 

Photometry 

Eppendorf µCuvette G1.0 

Photometry 

Photometry 

Photometry 



Coating 

Cuvette blank 

Layer thickness 

Light path height 

Material 

Minimum volume 


12.5 × 12.5 × 48 mm 

Hydrophobic surface coating 

≤ 0.1 A @ 230 nm, ≤0.05 A @ 260 nm 

1 mm 

8.5 mm 

Quartz glass and aluminum 

1.5 µL dsDNA, 3 µL protein 

180 nm – 2,000 nm 

Photometry 

Photometry 

Description 

The Eppendorf µCuvette G1.0 microliter measuring cell is the 

perfect tool for measuring high concentrations in the smallest 

volumes. The higher the sample concentration, the shorter 

the optical path length used to measure the sample. With an 

optical path length of only 1 mm, the µCuvette G1.0 features 

a light path that is ten times shorter than the light path on 

standard cuvettes. This allows nucleic acid concentrations 

to be measured with high reproducibility in a much higher 

concentration range without prior dilution. Self-absorption 

of the µCuvette G1.0 is very low, meaning that the entire 

measuring range of the photometer can be used. 

The µCuvette G1.0 has been exclusively developed for the 

Eppendorf BioPhotometer and Eppendorf BioSpectrometer. 


> > Microvolume measuring cell for measuring 1.5 - 10 µL 

sample volumes 

> > Low self-absorption (≤0.05 A @ 260 nm) 

> > One component hinged design, prevents the loss of 

individual parts 

> > Hydrophobic surface coating on quartz glass for precise 

formation and positioning of the sample volume 

> > A defined angle for folding the sides of the cuvette guarantees 

reproducible handling 

> > Simple cleaning to minimize the risk of sample carry-over 



Eppendorf µCuvette G1.0, Eppendorf microvolume measuring cell for Eppendorf 

6138000018 $2,374.00 

BioPhotometer and BioSpectrometer 

Eppendorf µCuvette G1.0 & BioPhotometer D30 (bundle), Eppendorf microvolume 6133000940 7,753.00 

measuring cell and BioPhotometer D30, 110 V/50 – 60 Hz 

Eppendorf µCuvette G1.0 & BioPhotometer plus (bundle), Eppendorf microvolume 6132000996 7,375.00 

measuring cell and BioPhotometer plus, 110 V/50 – 60 Hz 

Eppendorf µCuvette G1.0 & BioSpectrometer basic (bundle), Eppendorf microvolume 

measuring cell and BioSpectrometer basic, 110 V/50 – 60 Hz 

6135000923 9,498.00 

Photometry 

Applications 

> > Concentration determination of nucleic acids and proteins 

> > Measurement of high sample concentrations without 

prior dilution 

> > Low sample volume 







68 69 

Photometry 

Photometry 

Eppendorf PlateReader AF2200 

Photometry 

Photometry 




Compatible plates 

Detector 

460 × 462 × 270 mm 

14 kg 

6 to 384 wells, 

microvolume plate (Eppendorf µPlate G0.5) 

UV/VIS absorption: UV silicon photodiode 

Fluorescence intensity: multiplier 

Shortest measurement time 96-well plate (20 s), 384-well plate (30 s) 

Temperature control Ambient temperature + 5 °C up to 42 °C 

Light source 

UV Xenon flash lamp 

Absorption measuring range 0 A – 4.0 A 

Mode 

Two operating modes: 

UV/VIS absorption 

Fluorescence intensity: top/bottom 

Shaking 

Linear/orbital, amplitude can be selected from 1 – 6 mm in 0.5 mm increments 

Software 

PlateReader AF2200 application software, multi-user license, preprogrammed applications 

including evaluation 

Software compatibility 

Windows 7, XP or Vista 


UV/VIS absorption: 230 – 1,000 nm 

Fluorescence intensity: Ex: 230 – 600 nm, Em: 330 – 600 nm 

Photometry 

Photometry 

Photometry 

Description 

The PlateReader AF2200 is the ideal device for quantifying 

biomolecules such as nucleic acids and proteins, and 

measuring fluorescence-based assays in a plate format. Thanks 

to the large selection of filters, a wide variety of applications 

can be carried out in the UV/VIS range and a high number 

of fluorescence dyes can be detected. The device software 

features optimized, preprogrammed applications and a flexible 

selection of individual parameters. It also offers the option 

to freely program all parameters in order to meet special 

requirements. The PlateReader AF2200 unites the simplest 

programming and evaluation with maximum flexibility. 

For the most frequently used applications, Eppendorf offers 

two preconfigured filter slides that feature optimized filter 

assignment for the preprogrammed applications. Of course, 

freely configurable filter combinations are also available. 

Applications 

> > Quantification of biomolecules via absorption measurements 

in the UV/VIS range 

> > Fluorescence intensity for sensitive nucleic acid 

quantification, cell-based assays and other applications 

> > Preprogrammed applications for standard series (e.g., 

colorimetric protein assays, cell-based applications, etc.) 

> > Preprogrammed applications for the Eppendorf µPlate G0.5 

> > Preprogrammed applications with factor evaluation (for use 

with Eppendorf plates) 

> > Freely programmable parameters 


> > Dual-mode plate reader for UV/VIS absorption and 

fluorescence intensity 

> > Optimal preconfigured, and freely configurable filter 

combinations 

> > Preprogrammed applications for quick start and simple 

evaluation (factor or standard series) 

> > Results display in Excel format 

> > Clearly designed software with multi-user license 

> > Optional µPlate for measuring the smallest sample volumes 

using own application 

> > Temperature control and the option to shake the sample plate 



Eppendorf PlateReader AF2200, 110 V/50 – 60 Hz 6141000010 Inquire 

Eppendorf µPlate G0.5 & PlateReader AF2200 (bundle), Eppendorf microvolume plate 6141000922 Inquire 

and PlateReader AF2200, 100 V/50 – 60 Hz 

Eppendorf µPlate G0.5, Eppendorf microvolume plate for the Eppendorf PlateReader 6144000003 Inquire 

AF2200 

Fluorescence filter slide for PlateReader AF2200, preconfigured filter slide, optimized 6141070027 Inquire 

for the fluorescence dyes most frequently used in molecular biology and cell biology labs 

(360/465, 485/535, 485/595, 535/595) 

UV/VIS filter slide for PlateReader AF2200, preconfigured filter slide, optimized for 

applications in the UV and Vis range, 4 filters (260, 280, 340, 600 nm) 

6141070019 Inquire 

Photometry 







70 71 

Photometry 

Photometry 

Filter slides for PlateReader AF2200 

Photometry 

Photometry 


Filter wavelengths 

UV/VIS filter slide for 

PlateReader AF2200 

260 nm (5 nm) 

280 nm (5 nm) 

340 nm (10 nm) 

600 nm (10 nm) 

Fluorescence filter slide for PlateReader AF2200 

Excitation 

Emission 

360 nm (35 nm) 

485 nm (20 nm) 

485 nm (20 nm) 

535 nm (25 nm) 

465 nm (35 nm) 

535 nm (25 nm) 

595 nm (35 nm) 

595 nm (35 nm) 



UV/VIS filter slide for PlateReader AF2200, preconfigured filter slide, optimized for 6141070019 Inquire 

applications in the UV and Vis range, 4 filters (260, 280, 340, 600 nm) 

Fluorescence filter slide for PlateReader AF2200, preconfigured filter slide, optimized 

for the fluorescence dyes most frequently used in molecular biology and cell biology labs 

(360/465, 485/535, 485/595, 535/595) 

6141070027 Inquire 

Photometry 

Photometry 

Photometry 

Description 

For the most frequently used PlateReader AF2200 applications, 

Eppendorf offers two preconfigured filter slides with optimized 

filter assignment for preprogrammed applications. The filter 

slide design covers a wide spectrum, making it the perfect tool 

for a variety of applications. Compared to a freely configurable 

filter slide, these filters save you a large portion of the filter 

cost. 

Applications 

> > Preprogrammed applications including evaluation for 

concentration determination of nucleic acids and proteins via 

factor or standard 

> > Preprogrammed cell biology assays (viability and apoptosis) 

> > Simple programming of several applications 

> > Large selection of additional filters 


> > Optimized filter assignment for UV/VIS and fluorescence 

applications preprogrammed in the PlateReader AF2200 

> > Filter assignment covers a broad spectrum of UV/VIS or 

fluorescence applications 

> > Optimized arrangement in UV/VIS filter slide for molecular 

biology applications (nucleic acids, proteins, OD600) 

> > Optimized arrangement in fluorescence filter slide for 

molecular biology and cell biology applications 

> > Overlapping of spectra in fluorescence filter slide for optimal 

coverage of excitation and emission wavelengths 

Photometry 







72 73 

Photometry 

Photometry 

Eppendorf µPlate G0.5 

BioSpectrometer fluorescence reference filter set 

Description 

The BioSpectrometer fluorescence reference filter set is used 

for verifying photometric accuracy, wavelength systematic 

error, and fluorimetric precision (random error) and linearity 

on the BioSpectrometer fluorescence. 

Photometry 


> > All features of the BioSpectrometer UV/VIS reference filter set 

> > Additional filter for verifying fluorimetric precision (random 

error) and linearity 

> > Inspection protocol preprogrammed in device 

> > 8 filters (1 blank filter, 3 filters for UV/VIS wavelength 

systematic error and precision, 3 filters for UV/VIS 

photometric verification, 1 filter for fluorometric verification) 

Photometry 



Photometry 

Photometry 

Description 

As an optional accessory for the PlateReader AF2200, 

Eppendorf offers a microvolume plate that can be used to 

measure up to 16 samples in the microvolume scale. The 

Eppendorf µPlate G0.5 features simple handling and cleaning, 

and is used to measure high concentrations in the smallest 

volumes. The short optical path length of just 0.5 mm allows 

high nucleic acid or protein concentrations to be measured 

with high reproducibility and without prior dilution. Because 

the concentration is determined using a sample-specific factor, 

a standard series measurement is not required. 

Applications 

> > Concentration determination of nucleic acids and proteins 

> > Measurement of high sample concentrations without prior 

dilution 

> > Low sample volume 


> > Simple handling and cleaning 

> > Sample-specific factor for the concentration calculation 

eliminates the need for a standard series measurement 

> > Application for sample quantification is preprogrammed in 

the Eppendorf PlateReader AF2200 

> > Available for the Eppendorf PlateReader AF2200 



Eppendorf µPlate G0.5, Eppendorf microvolume plate for the Eppendorf PlateReader 6144000003 Inquire 

AF2200 

Eppendorf µPlate G0.5 & PlateReader AF2200 (bundle), Eppendorf microvolume plate 

and PlateReader AF2200, 110 V/50 – 60 Hz 

6141000922 Inquire 

BioSpectrometer fluorescence reference filter set, filter set for verifying photometric 

accuracy and wavelength systematic error (according to NIST) and verifying fluorimetric 

precision (random error) and linearity 

BioPhotometer D30 reference filter set 

6137928009 $3,752.00 

Description 

The secondary UV/VIS BioSpectrometer D30 reference 

filter set is used for verifying the photometric accuracy and 

wavelength systematic error on the BioPhotometer D30. 


> > Filter traceable to NIST 

> > Certificate with two year period of validity 

> > Filter recertification via Eppendorf (contact: Eppendorf 

Service) 

> > Inspection protocol preprogrammed in device 

> > Verification of wavelength accuracy and precision at 260 nm 

and 280 nm 

> > Verification of the photometric accuracy and precision for all 

BioPhotometer D30 wavelengths 

> > Contains 6 filters (1 blank filter, 2 filters for checking 

wavelength systematic error and precision, 3 filters for 

photometric verification) 



BioPhotometer D30 reference filter set, filter set for verifying photometric accuracy and 

wavelength systematic error (according to NIST) 

6133928004 $1,750.00 

Photometry 

Photometry 





74 75 

Cell Handling 

> > CO 2 

incubator shakers 76 

> > New Brunswick bioreactors 80 

> > DASGIP bioreactors 86 

30 years of competence in cell handling … 

It all began with successful cell microinjections, back in the 1980s. Those very cautious and tentative 

initial steps have become an indispensable part of modern day-to-day-scientific work – as have 

Eppendorf´s cell handling products. With the acquisition of New Brunswick in 2007 and DASGIP in 

January 2012, Eppendorf has expanded its competencies in this area even further, adding shakers, 

incubators and fermentors.

Cell Handling 

Cell Handling 

76 77 

Incubator shaker 


New Brunswick S41i 

Removable shelf lets you 

simultaneously shake suspension cells 

and incubate adherent cells under the 

same conditions 

Easy-to-clean seamless 

stainless steel chamber 

eliminates a potential 

source of contamination 





The advanced and intuitive touch screen 

controls not only provide a clear and accurate 

determination of the conditions your cells are 

exposed to but allows you to track performance 

over time as well as many additional features 

and functions 



Glass inner door allows 

viewing of the cultures without 

compromising sample and 

environmental integrity 

The heavy duty shaking mechanisms 

use 3 eccentric shafts providing stable, 

uniform and vibration-free motion 

along with the superb reliability that 

New Brunswick Shakers are known for 





Cell Handling 

Cell Handling 

78 79 



New Brunswick S41i 




Description 

The New Brunswick S41i is the only CO 2 

incubator with a 

New Brunswick Shaker inside. The S41i precisely controls 

temperature, shaking speed, CO 2 

and optionally O 2 

providing 

the ideal environment for growing mammalian, insect and 

plant suspension cultures. Accepts flasks up to 4 liters and 

includes a perforated shelf that incubate cultures at the same 

time and environment that you are shaking non-adherent 

suspension cells. The world famous triple eccentric drive 

provides stable, uniform and vibration free motion - connected 

to the shaking platform by four encapsulated rods, it’s located 

below the chamber ensuring that heat generated by the drive 

does not affect the critical environment within the chamber 

The intuitive touch screen display and controls allow you to 

operate and monitor performance. 

Applications 

> > Culturing of non-adherent suspension cells requiring gentle 

agitation 

> > Incubating adherent cells on the shelf included with the 

unit…adding a 2nd shelf and using the shaking platform as a 

3rd shelf 

> > Adherent cells such as Stem cells that require both incubation 

and periodic gentle shaking 


> > Temperature range from 4 °C above ambient to 50 °C 

> > Shaking speeds between 25 – 400 rpm (±1 % full scale), 

25 – 300 rpm if stacked, provides versatility for culturing a 

wide range of cell types. 

> > Large 35.6 x 61 cm / 24 x 14 in platform provides both size 

and flexibility with cultures 

> > Single or double stacked for incresed capacity 

> > Inner and outer door saves valuable gases while maintaining 

the perfect environment for your cells 

> > High Temperature Disinfection (HTD) protects against 

bacterial contamination 

> > Intuitive touch screen controls provides for precise control of 

the culturing environment 

> > Optional perforated shelf allows for static incubation at the 

same time as shaking 


Weight w/o accessories 152 kg / 335 lb 

Balanced drive mechanism Triple eccentric drive mechanism with counter balancing to provide vibration free operation 

cETLus and CE marked yes 

Platform size 

61.2 x 35.6 cm / 24 x 14 in 

Speed range 

25-400 rpm 

Temperature uniformity +/- 0.25 °C 



New Brunswick S41i, CO 2 

incubator shaker with inner shelf and touch screen control, S41i-120-0100 $15,600.00 

120 V/60 Hz, Orbit diameter 2.5 cm / 1 in 

Interchangeable platform for New Brunswick S41i, 35.6 × 61 cm / 24 x 14 in 

Universal platform M1334-9920 845.00 

125 mL Erlenmeyer flask dedicated platform, comes complete with 32 clamps installed M1334-9921 970.00 



1 L Erlenmeyer flask dedicated platform, comes complete with 11 clamps installed M1334-9924 885.00 


2.8 L Erlenmeyer flask dedicated platform, comes complete with 3 clamps installed M1334-9926 885.00 


Stacking kit, allows up to 2 each New Brunswick S41i shakers to be stacked one on top P0628-6502 1,450.00 

of the other 

Additional shelf for New Brunswick S41i, perforated, can easily be added to the 

New Brunswick S41i for static cell culture of adherent cells 

P0628-6181 227.40 








Cell Handling 

Cell Handling 

80 81 

Bioprocessing 

Bioprocessing 

New Brunswick CelliGen ® BLU 



New Brunswick CelliGen ® BLU 

Bioprocessing 

Bioprocessing 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), M1374-120-H0A Inquire 

no scale 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 Rotameter, no scale M1374-120-H0M Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), scale M1374-120-HSA Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 Rotameter, scale M1374-120-HSM Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), M1374-120-L0A Inquire 

no scale 

120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 Rotameter, no scale M1374-120-L0M Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), scale M1374-120-LSA Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 Rotameter, scale M1374-120-LSM Inquire 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), M1374-120-H0AV Inquire 

no scale, validation included 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 Rotameter, no scale, 

M1374-120-H0MV Inquire 

validation included 

120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), scale, M1374-120-HSAV Inquire 


120 V, 60 Hz, Sparge: 3 TMFC (0.04 - 20 SLPM), Overlay: 1 Rotameter, scale, 

M1374-120-HSMV Inquire 


120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), M1374-120-L0AV Inquire 

no scale, validation included 

120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 Rotameter, no scale, 

M1374-120-L0MV Inquire 


120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 TMFC (0.01 - 5 SLPM), scale, M1374-120-LSAV Inquire 


120 V, 60 Hz, Sparge: 3 TMFC (0.002 - 1 SLPM), Overlay: 1 Rotameter, scale, 


M1374-120-LSMV Inquire 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Description 

The New Brunswick CelliGen ® BLU is a benchtop bioreactor 

that combines all the convenience of single-use technology 

with the trusted performance, advanced process management 

and scalability of a stirred-tank design, making it ideal for 

research through production. 


> > Sophisticated RPC (Reactor Process Controller) software 

controls up to 32 process parameters, trends up to 8 loops, 

and provides built-in security 

> > Interchangeable, single-use vessels with rigid-walled design 

are offered in 5.0, 14.0, and 50.0 liter (total volume) capacities 

> > 5 L Vessel with Packed-bed impeller available, pre-loaded 

with 150 g of Fibra-Cel ® disks 

> > Integrated control of up to 8 gases for sparge and gas overlay, 

available in high and low flow TMFC configurations 

> > Systems include 3 integrated pumps; magnetic-drive motor; 

temperature, pH, and DO control; 3 or 4-gas mixing; 7 analog 

Input/Output connections 

> > Industrial touchscreen interface with adjustable-position, 

38.1 cm (15 in) color display 

> > Vessel components are constructed from USP Class VI and 

animal free materials, making them acceptable for GMP 

environments 

> > Single-Use vessels eliminate autoclaving, improve turnaround 

time, simplify validation and reduce cost 

> > Vessels are assembled with sprage, overlay, and exhaust 

filters as well as penetrations for pH, DO, temp, liquid 

additions, sampling and harvesting 

> > Pitched-blade impeller available on all vessel sizes 

> > Vessel and cabinet validation available upon request 

New Brunswick CelliGen ® BLU Single-Use Vessels 



New Brunswick CelliGen ® BLU Single-Use Vessels, Microsparge 

5L Vessel, 1-pack M1363-0125 Inquire 




5L Packed Bed Vessel, 1-pack M1363-0119 Inquire 




New Brunswick CelliGen ® BLU Single-Use Vessels, Macrosparge 









Bioprocessing 





Cell Handling 

Cell Handling 

82 83 

Bioprocessing 

Bioprocessing 

New Brunswick CelliGen ® BLU Vessel Kits 

New Brunswick CelliGen ® BLU Adapter Kits 

Bioprocessing 

Bioprocessing 

Bioprocessing 



New Brunswick CelliGen ® BLU Vessel Kits 

5L Vessel Kit (120 V) M1363-0105 $1,538.00 

14L Vessel Kit (120 V) M1363-0114 1,794.00 

5L Packed Bed Vessel Kit (120 V) M1363-0108 1,569.00 

50L Vessel Kit (120 V) M1374-0151 3,070.00 

New Brunswick CelliGen ® BLU Accessories 



500 mL Media/Supplement Addition Kit P0640-8860 $ 218.00 

1 L Media/Supplement Addition Kit P0640-8861 232.00 



“Y“ Connector for custom manifolds P0620-0947 194.00 

Vessel Validation M1374-0061 5,130.00 

Starter Kit, 5/14L Vessel M1374-0111 1,540.00 

Starter Kit, 50L Vessel M1374-0112 1,790.00 

Pressure Regulator Kit M1363-5002 769.00 

Needle-Free Syringe, pack of 10 M1363-9910 54.00 

Polysulfone quick-connect P0240-2670 4.70 

Heat Blanket, 5L Vessel (120 V) M1363-8012 513.00 



RTD and cable, 5L Vessel M1363-8025 295.00 



Optical pH Probe and cable, 5L Vessel P0300-2371 157.00 

Optical pH Probe and cable, 14L Vessel P0300-2370 277.00 

Optical pH Probe and cable, 50L Vessel P0300-2374 1,023.00 

DO Probe, 5L Vessel P0720-6526 741.00 

DO Probe, 14L Vessel P0720-6525 772.00 

DO Probe, 50L Vessel P0720-6529 1,794.00 

DO Probe, 5L Packed-Bed Vessel P0720-6280 1,433.00 

DO Probe cable P0720-2336 483.00 

pH Probe and Cable, 5L Packed Bed Vessel P0720-5588 584.00 

High Flow Replacement Sparge Drawer M1374-0114 Inquire 

Low Flow Replacement Sparge Drawer M1374-0113 Inquire 

Optical pH Probe and cable, 5L Packed-Bed Vessel P0300-2372 267.00 

RTD and Cable, 5L Packed-Bed Vessel M1363-8028 308.00 

Description 

New Brunswick CelliGen ® BLU Adapter Kits convert existing autoclavable bioreactor controllers for use with New Brunswick 

CelliGen ® BLU single-use vessels. 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & CelliGen ® 310 

> > Adapts New Brunswick CelliGen ® 310 autoclavable controller for use 

with New Brunswick CelliGen ® BLU single-use vessels 

> > Provides all the benefits of single use technology with minimal upfront 

investment 

> > Easy to install kits provide all the necessary equipment for conversion 

> > Includes: Vessel heat blanket, relief valve assembly (2), exhaust heat 

blanket, heat blanket conversion drawer, motor adapter, spare tubing. 

> > CelliGen ® BLU Vessels sold separately 


Adapter Kit: CelliGen ® BLU Single-Use Vessel & BioFlo ® / CelliGen ® 115 

> > Adapts New Brunswick BioFlo ® / CelliGen ® 115 autoclavable controller 

for use with New Brunswick CelliGen ® BLU single-use vessels 


investment 


> > Includes: Vessel heat blanket, pressure relief valve assembly (2), exhaust 

tube heat blanket, spare tubing 

> > Requires: CelliGen ® 115 Magnetic Drive Motor, not included 

(Catalog No. M1369-3130). 



Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ADI 1025 

Bio Console 

> > Adapts Applikon ® ADI 1025 Bio Console autoclavable cell culture systems 

for use with New Brunswick CelliGen ® BLU 5.0 and 14.0L vessels 


investment 



tube heat blanket, RTD, motor adapter, spare tubing. 



Bioprocessing 

Bioprocessing 

Bioprocessing 





Cell Handling 

Cell Handling 

84 85 

Bioprocessing 

Bioprocessing 

New Brunswick CelliGen ® BLU Adapter Kits 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Description 

New Brunswick CelliGen ® BLU Adapter Kits convert existing autoclavable bioreactor controllers for use with New Brunswick 

CelliGen ® BLU single-use vessels. 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Sartorius BioStat ® 

B-Plus (Left-Hand) 

> > Adapts Sartorius ® B-plus autoclavable cell culture systems for use with 

New Brunswick CelliGen ® BLU 5.0 and 14.0L vessels 


investment 


> > Includes: Vessel heat blanket, relief valve assembly (2), exhaust heat 

blanket, RTD, heat blanket conversion drawer, motor adapter, spare 

tubing. 



Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ez-Control 

> > Adapts Applikon ® ez-Control autoclavable cell culture systems for use 

with New Brunswick CelliGen ® BLU 5.0 and 14.0L vessels 


investment 



tube heat blanket, RTD, motor adapter, spare tubing. 



CelliGen ® BLU Adapter Kit - Op-76 Optical pH Module 



Adapter Kit: CelliGen ® BLU Single-Use Vessel & BioFlo ® / CelliGen ® 115 

5L vessels, 120 V M1376-9941 $1,200.00 

14L vessels, 120 V M1376-9942 1,410.00 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & CelliGen ® 310 

5L vessels, 120 V M1376-9927 1,730.00 

14L vessels, 120 V M1376-9928 1,930.00 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ADI 1025 Bio Console 

5L vessels, 120 V M1376-9921 1,410.00 

14L vessels, 120 V M1376-9922 1,620.00 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Applikon ez-Control 

5L vessels, 120 V M1376-9944 1,640.00 

14L vessels, 120 V M1376-9943 1,640.00 

Adapter Kit: CelliGen ® BLU Single-Use Vessel & Sartorius BioStat ® B-Plus (Left-Hand) 

5L vessels, 120 V M1376-9931 2,350.00 

14L vessels, 120 V M1376-9932 2,350.00 

CelliGen ® BLU Adapter Kit - Op-76 Optical pH Module M1376-1001 8,150.00 

5L pH Probe and cable P0300-2371 157.00 

14L pH Probe and cable P0300-2370 277.00 

Bioprocessing 

Bioprocessing 

Bioprocessing 

> > Eliminates pH probe autoclaving and under-the hood insertion to further 

reduce setup time and costs. 

> > Users can now experience all the benefits of our single-use technology 

without replacing their existing autoclavable bioreactor controller. 

> > Intended for use with New Brunswick CelliGen ® BLU Adapter Kits 

> > Compatible with Applikon, Sartorius and New Brunswick autoclavable 

cell culture systems 


> > Select pH probe and cable size M1376-1001 





Cell Handling 

Cell Handling 

86 87 

Bioprocessing 

Bioprocessing 

DASGIP ® Mini Bioreactor System DASbox ® 

DASGIP ® Parallel Bioreactor System 

for CelliGen ® BLU 5L Vessel 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Description 

The DASGIP DASbox is a new and unique mini bioreactor 

system suitable for microbial fermentation and cell cultivation. 

It was designed as a 4-fold modular system with up to 32 or 

more parallel operating bioreactors. With working volumes 

of 60 – 250 mL the DASbox is the optimal tool for advanced 

process development and Design of Experiments (DoE) 

applications. All critical process parameters can be precisely 

controlled. Liquid-free temperature control and exhaust gas 

condensation satisfy users with easy handling. 

In addition to using industry standard glass bioreactors the 

DASbox can be equipped with the new DASbox single-use 

vessel (100 – 250 mL working volume), a fully instrumented 

single-use mini bioreactor. 


> > Parallel set-up of 4 up to 32 or even more bioreactors 

> > Excellent scalability and reproducibility in both microbial and 

cell culture applications 

> > Supports industry standard glass bioreactors (DASGIP Mini 

Bioreactor) as well as DASbox single-use vessels 

> > Small working volumes, 60 – 250 mL (100 – 250 mL with 

single-use vessel) save cell material, media and supplements 

> > Extraordinarily compact system with a footprint of only 

7.5 cm (3 in) wide benchspace per vessel 

> > Individual temperature control with liquid-free heating and 

cooling 

> > Liquid-free exhaust gas condenser with easy handling by 

automatic click in activation and click out deactivation mode 

> > LC display with key process parameters and integrated alarm 

function simplifies monitoring 

> > Fully mass flow-controlled gas mixing with individual gas 

mixture from air, O 2 

, CO 2 

and N 2 

, each directable either to 

headspace or submerged 

> > Standard probes for precise measurement and control of 

temperature, pH, DO and level 

> > Precision miniature variable speed pumps, continuous flow 

rates down to 0.3 mL/h 

> > Magnet-coupled (single-use) or directly coupled (reusable) 

overhead drives, up- or downflow selectable 

Description 

The New Brunswick CelliGen BLU 5L single-use vessel is 

a fully instrumented single-use bioreactor. Operating the 

DASGIP Control Software and the various DASGIP modules 

for monitoring and control with CelliGen BLU bioreactors 

scientists working in cell culture process development 

make use of DASGIP’s well-established process control and 

comprehensive information management while benefitting 

from the advantages of single-use solutions. Parallel operation 

and calibration, powerful recipe management and automated 

Microsoft Excel reporting accelerates process development. 

DoE approaches and QbD-conform proceeding are supported. 


> > Fully instrumented single-use bioreactor for accelerated 

process development in cell culture applications 

> > Parallel operation and calibration, integrated recipe 

management and Microsoft Excel reporting 

> > Software suite DASGIP DASware offers matchless options for 

data management, interconnectivity with 3 rd party lab devices, 

remote control and DoE 

> > With working volumes of 1.25 – 3.75 L the CelliGen BLU 5L is 

the perfect tool for process development as well as lab-scale 

production procedures 

> > Liquid-free exhaust gas condenser with easy handling by 

automatic click in activation and click out deactivation mode 

> > Magnet-coupled overhead drive reduces the risk of 

contamination, pitched blade impeller, up- or downflow with 

30 – 200 rpm 

> > Fully mass flow-controlled gas mixing of air, O 2 

, CO 2 

and N 2 

> > Operating with industry standard probes for precise 

measurement and control of temperature, pH and DO 

> > Optionally, the CelliGen BLU is delivered with a PreSens pH 

optode readily installed, fully supported by the control system 

> > Variable speed-controlled peristaltic pumps for multiple fluid 

transfers, continuous flow rates between 0.3 and 420 mL/h 

Bioprocessing 

Bioprocessing 



DASGIP ® Mini Bioreactor System DASbox ® for Cell Culture Applications 

120 V, 50 – 60 Hz, with 4 vessels, max. 5 sL/h gassing, for cell culture applications 76DX04CC Inquire 

120 V, 50 – 60 Hz, with 8 vessels, max. 5 sL/h gassing, for cell culture applications 76DX08CC Inquire 

DASGIP ® Mini Bioreactor System DASbox ® for Microbial Applications 

120 V, 50 – 60 Hz, with 4 vessels, max. 25 sL/h gassing, for microbial applications 76DX04MB Inquire 

120 V, 50 – 60 Hz, with 8 vessels, max. 25 sL/h gassing, for microbial applications 76DX08MB Inquire 

DASGIP ® Mini Bioreactor System DASbox ® with BioBLU 0.4c single-use vessel 

120 V, 50 – 60 Hz, with 4 single-use vessels, max. 5 sL/h gassing, for cell culture applications 76DX04CCSU Inquire 

120 V, 50 – 60 Hz, with 8 single-use vessels, max. 5 sL/h gassing, for cell culture applications 76DX08CCSU Inquire 



DASGIP ® Parallel Bioreactor System for CelliGen ® BLU 5L Vessel, 

for cell culture applications 

for 4 New Brunswick CelliGen ® BLU 5L single-use vessels 76CF04CCSU05 Inquire 

for 8 New Brunswick CelliGen ® BLU 5L single-use vessels 76CF08CCSU05 Inquire 





Cell Handling 

Cell Handling 

88 89 

Bioprocessing 

Bioprocessing 

DASGIP ® BioBLU 0.4c Single-Use Vessel 

DASware ® migrate for CelliGen ® and BioFlo ® controllers 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Bioprocessing 

Description 

The BioBLU 0.4c is a single-use stirred tank mini bioreactor 

to accelerate process development in biopharmaceutical or 

process screening for cell culture applications. With working 

volumes of 100 – 250 mL it is the perfect tool for early process 

development procedures. All critical process parameters in 

applications like cell line selection or protein production can 

be precisely controlled. The unique liquid-free exhaust gas 

condenser reduces negative effects on sensitive cell cultures 

caused by change of medium osmolality and efficiently 

prevents blocking of off-gas filter. The BioBLU 0.4c singleuse 

vessel is used with the DASGIP DASbox parallel mini 

bioreactor system. 


> > Small working volumes of 100 – 250 mL save cell material, 

media and supplements 

> > Used with the DASbox mini bioreactor system for parallel setup 

of 4 up to 32 bioreactors 

> > Liquid-free exhaust gas condenser eliminating the need of a 

chiller system 

> > Magnet-coupled overhead drive reduces the risk of 

contamination, pitched blade impeller, up- or downflow with 

30 – 500 rpm 

> > Headplate with 2x PG 13.5 ports, 4x integrated long dip 

tubes, 2x integrated short dip tubes, one thermowell, DO 

sensor port with permeable membrane 

> > Operating with industry standard probes for precise 

measurement and control of temperature, pH, DO 

> > Vessels are pre-sterilized and includes all hoses and fillers 

Description 

DASGIP’s software DASware migrate provides the ability 

to operate a set of Eppendorfs New Brunswick BioFlo/ 

CelliGen 115 and 310 as well as CelliGen BLU Bioreactor 

units collectively - including shared recipe-, process data- and 

information management. All relevant process parameters can 

be simultaneously controlled, monitored and online visualized 

within a single user interface. Thereby, DASware migrate for 

CelliGen and BioFlo units provides all crucial advantages of the 

DASware bioprocessing software to users in microbiology and 

cell culture. Utilizing this solution enables access to DASGIP’s 

advanced control features and powerful Microsoft Excel 

reporting. 


> > Grouped operation of several BioFlo/CelliGen 115 and 310 as 

well as CelliGen BLU Bioreactor units, precisely controlled via 

DASGIP Control Software 

> > Access to the complete DASware software solution suite 

> > Comprehensive information management and process data 

historian, shared recipes 

> > Powerful Microsoft Excel report generator provides recipe 

information, process information as well as event reporting 

> > Facilitates Design of Experiments (DoE) approaches, 

process automation as well as data management and storage 

consistent to the FDA’s QbD guidelines 

> > Allows for integration of DASGIP off-gas analyzer GA4, 

biomass monitor OD4 and precision multi pumps MP8 into 

New Brunswick bioreactor controllers 

> > Enables integration of 3 rd party autosamplers and analyzers 

with direct feedback from the system according to online 

measured analytical data 

> > Optionally, remote control of bioprocesses via PC, Notebook, 

iPhone, iPod touch or iPad 

Bioprocessing 

Bioprocessing 



BioBLU 0.4c Single-Use Vessel, for cell culture applications, 4 vessel package 78903501 Inquire 



DASware ® migrate for CelliGen ® and BioFlo ® units, DASGIP Control System for 

New Brunswick BioFlo/CelliGen 

4 units 76DG04CSNB Inquire 

8 units 76DG08CSNB Inquire 





Application support 

North America: 800-645-3050, menu option 2 

techserv@eppendorf.com 

Eppendorf North America, Inc. 

Phone: 800-645-3050 

Email: info@eppendorf.com 

Eppendorf Canada Ltd. 

Phone: 800-263-8715 

Email: canada@eppendorf.com 

www.eppendorfna.com 

ENA.C1.0201.A.US.13 © 2013 Eppendorf AG. Eppendorf ® is a registered trademark of Eppendorf AG. Product appearance, specifications, and/or prices are subject to change without notice.

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