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324 Heegaard et al. Fig. 7. Drug-protein binding studied by capillary electrophoresis-frontal analysis (CE-FA) in 0.067 M phosphate buffer, pH 7.4. (A) Electropherograms of 391 M warfarin with or without 65 M human serum albumin (HSA) (—, warfarin with HSA; ———; (e) warfarin without HSA;—– , (d) HSA blank). Experiments were performed on a Hewlett-Packard 3D CE-instrument. Conditions: Uncoated fused silica capillary (48.5 cm × 50 m i.d., 40 cm effective length); applied voltage +10 kV (∼ 46 A);
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Affinity Chromatography second edit
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METHODS IN MOLECULAR BIOLOGY TM Aff
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To Tina, Emmanuella, Natalie, and A
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viii Preface Affinity tags for puri
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x Contents 10. Immobilized Metal Io
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xii Contributors Tzong-Hsien Lee
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2 Roque and Lowe cell extract conta
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4 Roque and Lowe groups critical in
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6 Roque and Lowe of reinforcement e
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8 Roque and Lowe unknown factors ar
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10 Roque and Lowe receptor domains
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12 Roque and Lowe A further issue o
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14 Roque and Lowe transgenic system
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16 Roque and Lowe 6. Arsenis, C. an
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18 Roque and Lowe 39. Burton, S.J.,
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20 Roque and Lowe 71. Bhikhabhai, R
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I Various Modes of Affinity Chromat
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26 Charlton and Zachariou Since the
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28 Charlton and Zachariou 5. Equili
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30 Charlton and Zachariou 9. Elute
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32 Charlton and Zachariou 3.3. Puri
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34 Charlton and Zachariou could als
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3 Affinity Precipitation of Protein
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Affinity Precipitation of Proteins
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4 Immunoaffinity Chromatography Stu
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Immunoaffinity Chromatography 55 2.
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Immunoaffinity Chromatography 57 A
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Immunoaffinity Chromatography 59 ab
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5 Dye Ligand Chromatography Stuart
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Dye Ligand Chromatography 63 Develo
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Dye Ligand Chromatography 65 6. Mis
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Dye Ligand Chromatography 67 Fig. 1
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Dye Ligand Chromatography 69 6. Sec
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72 Tugcu chromatography, the sorpti
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74 Tugcu non-specific interactions,
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76 Tugcu the salt counter ions on t
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78 Tugcu On a plot of log K versus
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80 Tugcu Figure 2B illustrates a ty
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82 Tugcu 2.5. Running Displacement
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84 Tugcu then the operating conditi
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86 Tugcu Table 1 Trobleshooting for
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88 Tugcu 23. Torres, A. R. and Pete
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II Affinity Chromatography Using Pu
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94 Roque and Lowe market, with 14 F
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96 Roque and Lowe and RasMol V2.7.1
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98 Roque and Lowe house using, for
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100 Roque and Lowe Gly71 and Gly72)
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102 Roque and Lowe dissolved in ace
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104 Roque and Lowe equilibration bu
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106 Roque and Lowe of color. Purple
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108 Roque and Lowe 5. Fassina, G.,
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8 Phage Display of Peptides in Liga
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Phage Display of Peptides in Ligand
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9 Preparation, Analysis and Use of
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Preparation, Analysis and Use of an
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10 Immobilized Metal Ion Affinity C
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IMAC of Histidine-Tagged Fusion Pro
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152 Godat et al. tag. HQ-tagged pro
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154 Godat et al. 2. NaCl (5 M). 3.
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156 Godat et al. 4. Invert tube to
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158 Godat et al. 3. Sonicate sample
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160 Godat et al. the above protocol
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162 Godat et al. 3.7.1. Purificatio
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164 Godat et al. 3. Adding 200 mM N
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166 Godat et al. 4. The MagneHis Ni
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168 Godat et al. 22. Lin, D., Tabb,
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170 Pattenden and Thomas 1. Introdu
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172 Pattenden and Thomas functions
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174 Pattenden and Thomas of the mal
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176 Pattenden and Thomas necessary.
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178 Pattenden and Thomas 2.4. Amylo
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180 Pattenden and Thomas 9. Thoroug
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182 Pattenden and Thomas 8. Collect
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184 Pattenden and Thomas binding ef
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186 Pattenden and Thomas 15. Cells
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188 Pattenden and Thomas 23. Martin
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13 Methods for Detection of Protein
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Detection of Protein-Protein and Pr
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212 Charlton recognition sequence,
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214 Charlton when selecting Factor
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216 Charlton 2. Materials 2.1. Reag
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218 Charlton (see Notes 12 and 13).
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220 Charlton 3.3. Cleavage of Fusio
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222 Charlton 3. The catalytic mecha
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224 Charlton may dictate. However,
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226 Charlton 22. The full-length fu
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228 Charlton 22. Lien, S., Milner,
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230 Arnau et al. downstream process
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232 Arnau et al. Fig. 1. Overview o
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234 Arnau et al. Fig. 2. The pQE-1
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236 Arnau et al. 2.3.3. Step B Buff
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238 Arnau et al. Fig. 3. Immobilize
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240 Arnau et al. 6. Collect the flo
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242 Arnau et al. 4. Desalting is ne
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III Various Applications of Affinit
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248 Galaev and Mattiasson in biotec
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250 Galaev and Mattiasson 2.4. Sepa
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252 Galaev and Mattiasson 3.4. Sepa
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254 Galaev and Mattiasson ensure el
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17 Monolithic Bioreactors for Macro
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Monolithic Bioreactors for Macromol
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18 Plasmid DNA Purification Via the
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Plasmid DNA Purification 277 are pr
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Plasmid DNA Purification 279 5. Dec
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Plasmid DNA Purification 281 Initia
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Plasmid DNA Purification 283 8. Bou
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286 Tchaga proteins is variable. Ho
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288 Tchaga residue is buried and is
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290 Tchaga 3. Centrifuge the tube a
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292 Tchaga 5. Use the BCA Protein A
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294 Tchaga 24. Figeys D., Gygi S.P.
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296 Lee and Aguilar The primary dif
Page 590: 298 Lee and Aguilar 2.2. Equipment
Page 594: 300 Lee and Aguilar Abs 280 PLA 2 A
Page 598: 302 Lee and Aguilar 9. Pidgeon, C.,
Page 602: 304 Heegaard et al. 1. Introduction
Page 606: Table 1 CE Methods and Their Corres
Page 610: 308 Heegaard et al. Fig. 1. Images
Page 614: 310 Heegaard et al. characteristics
Page 618: 312 Heegaard et al. sample. A final
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Page 626: 316 Heegaard et al. parameters extr
Page 630: 318 Heegaard et al. f M s 5.0 0.01
Page 634: 320 Heegaard et al. where AL is the
Page 638: 322 Heegaard et al. Fig. 6. Mobilit
Page 644: Affinity Capillary Electrophoresis
Page 672: Index Adsorption isotherm, 75, 84 A
Page 676: Index 341 Genenase I, 170, 214, 215
Page 680: Index 343 Saccharomyces cerevisae,
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