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Table 1
CE Methods and Their Corresponding Acronyms Used for Characterization of Molecular Interactions a
Name Sample Electrophoresis buffer Quantitation parameter
Mobility shift assays
Mobility shift ACE (100) Analyte Ligand added Shift in mobility of analyte
Partial-filling ACE (84,101,101) Analyte Ligand added Shift in mobility of analyte
Vacancy affinity capillary
electrophoresis (VACE) (102)
Capillary affinity gel electrophoresis
(CAGE) (103)
Neat buffer Analyte + ligand
added
Analyte Ligand immobilized
in gel
Shift in mobility of analyte
(vacancy peak)
Shift in mobility of analyte
Hummel–Dreyer principle (104,105) Analyte + ligand Ligand added Peak area corresponding to
complex concentration
Vacancy peak analysis (VP) (104) Neat buffer Analyte + ligand
added
(vacancy peak)
Peak area of analyte
vacancy peak
Pre-equilibrium (pre-eq) assays
Pre-eq CZE (106) Analyte + ligand Neat buffer Peak area
Capillary electrophoresis frontal
Analyte + ligand Neat buffer Analyte plateau height
analysis (CE-FA) (9,104)
Frontal analysis continuous capillary
electrophoresis (FACCE) (107)
Affinity probe capillary
electrophoresis (APCE) (108,109)
Analyte + ligand Neat buffer Analyte plateau height
Analyte + ligand 1 Neat buffer or ligand
2 added
Peak area
CE, capillary electrophoresis; ACE, affinity CE.
a See Note 1 for alternative names of the methods.