View - ResearchGate

More documents

Recommendations

Info

Affinity Chromatography of Phosphorylated Proteins 289 Fig. 2. Overview of the purification procedure with Clontech’s PMAC Phosphoproteins Enrichment Kit. 3.1. Extracting Proteins from Cells 1. Wash 50–150 mg of cells three times with 20 vol of phosphate-buffered saline (PBS) by centrifuging at 500 × g in a pre-weighed centrifuge tube (see Note 2). 2. After washing, centrifuge cells as above and then decant the supernatant and aspirate the residual liquid.
Page 2:
Affinity Chromatography second edit
Page 6:
METHODS IN MOLECULAR BIOLOGY TM Aff
Page 10:
To Tina, Emmanuella, Natalie, and A
Page 14:
viii Preface Affinity tags for puri
Page 18:
x Contents 10. Immobilized Metal Io
Page 22:
xii Contributors Tzong-Hsien Lee
Page 26:
2 Roque and Lowe cell extract conta
Page 30:
4 Roque and Lowe groups critical in
Page 34:
6 Roque and Lowe of reinforcement e
Page 38:
8 Roque and Lowe unknown factors ar
Page 42:
10 Roque and Lowe receptor domains
Page 46:
12 Roque and Lowe A further issue o
Page 50:
14 Roque and Lowe transgenic system
Page 54:
16 Roque and Lowe 6. Arsenis, C. an
Page 58:
18 Roque and Lowe 39. Burton, S.J.,
Page 62:
20 Roque and Lowe 71. Bhikhabhai, R
Page 66:
I Various Modes of Affinity Chromat
Page 70:
26 Charlton and Zachariou Since the
Page 74:
28 Charlton and Zachariou 5. Equili
Page 78:
30 Charlton and Zachariou 9. Elute
Page 82:
32 Charlton and Zachariou 3.3. Puri
Page 86:
34 Charlton and Zachariou could als
Page 90:
3 Affinity Precipitation of Protein
Page 94:
Affinity Precipitation of Proteins
Page 98:
Page 102:
Page 106:
Page 110:
Page 114:
Page 118:
Page 122:
4 Immunoaffinity Chromatography Stu
Page 126:
Immunoaffinity Chromatography 55 2.
Page 130:
Immunoaffinity Chromatography 57 A
Page 134:
Immunoaffinity Chromatography 59 ab
Page 138:
5 Dye Ligand Chromatography Stuart
Page 142:
Dye Ligand Chromatography 63 Develo
Page 146:
Dye Ligand Chromatography 65 6. Mis
Page 150:
Dye Ligand Chromatography 67 Fig. 1
Page 154:
Dye Ligand Chromatography 69 6. Sec
Page 158:
72 Tugcu chromatography, the sorpti
Page 162:
74 Tugcu non-specific interactions,
Page 166:
76 Tugcu the salt counter ions on t
Page 170:
78 Tugcu On a plot of log K versus
Page 174:
80 Tugcu Figure 2B illustrates a ty
Page 178:
82 Tugcu 2.5. Running Displacement
Page 182:
84 Tugcu then the operating conditi
Page 186:
86 Tugcu Table 1 Trobleshooting for
Page 190:
88 Tugcu 23. Torres, A. R. and Pete
Page 194:
II Affinity Chromatography Using Pu
Page 198:
94 Roque and Lowe market, with 14 F
Page 202:
96 Roque and Lowe and RasMol V2.7.1
Page 206:
98 Roque and Lowe house using, for
Page 210:
100 Roque and Lowe Gly71 and Gly72)
Page 214:
102 Roque and Lowe dissolved in ace
Page 218:
104 Roque and Lowe equilibration bu
Page 222:
106 Roque and Lowe of color. Purple
Page 226:
108 Roque and Lowe 5. Fassina, G.,
Page 230:
8 Phage Display of Peptides in Liga
Page 234:
Phage Display of Peptides in Ligand
Page 238:
Page 242:
Page 246:
Page 250:
Page 254:
Page 258:
9 Preparation, Analysis and Use of
Page 262:
Preparation, Analysis and Use of an
Page 266:
Page 270:
Page 274:
Page 278:
Page 282:
10 Immobilized Metal Ion Affinity C
Page 286:
IMAC of Histidine-Tagged Fusion Pro
Page 290:
Page 294:
Page 298:
Page 302:
Page 306:
Page 310:
152 Godat et al. tag. HQ-tagged pro
Page 314:
154 Godat et al. 2. NaCl (5 M). 3.
Page 318:
156 Godat et al. 4. Invert tube to
Page 322:
158 Godat et al. 3. Sonicate sample
Page 326:
160 Godat et al. the above protocol
Page 330:
162 Godat et al. 3.7.1. Purificatio
Page 334:
164 Godat et al. 3. Adding 200 mM N
Page 338:
166 Godat et al. 4. The MagneHis Ni
Page 342:
168 Godat et al. 22. Lin, D., Tabb,
Page 346:
170 Pattenden and Thomas 1. Introdu
Page 350:
172 Pattenden and Thomas functions
Page 354:
174 Pattenden and Thomas of the mal
Page 358:
176 Pattenden and Thomas necessary.
Page 362:
178 Pattenden and Thomas 2.4. Amylo
Page 366:
180 Pattenden and Thomas 9. Thoroug
Page 370:
182 Pattenden and Thomas 8. Collect
Page 374:
184 Pattenden and Thomas binding ef
Page 378:
186 Pattenden and Thomas 15. Cells
Page 382:
188 Pattenden and Thomas 23. Martin
Page 386:
13 Methods for Detection of Protein
Page 390:
Detection of Protein-Protein and Pr
Page 394:
Page 398:
Page 402:
Page 406:
Page 410:
Page 414:
Page 418:
Page 422:
Page 426:
212 Charlton recognition sequence,
Page 430:
214 Charlton when selecting Factor
Page 434:
216 Charlton 2. Materials 2.1. Reag
Page 438:
218 Charlton (see Notes 12 and 13).
Page 442:
220 Charlton 3.3. Cleavage of Fusio
Page 446:
222 Charlton 3. The catalytic mecha
Page 450:
224 Charlton may dictate. However,
Page 454:
226 Charlton 22. The full-length fu
Page 458:
228 Charlton 22. Lien, S., Milner,
Page 462:
230 Arnau et al. downstream process
Page 466:
232 Arnau et al. Fig. 1. Overview o
Page 470:
234 Arnau et al. Fig. 2. The pQE-1
Page 474:
236 Arnau et al. 2.3.3. Step B Buff
Page 478:
238 Arnau et al. Fig. 3. Immobilize
Page 482:
240 Arnau et al. 6. Collect the flo
Page 486:
242 Arnau et al. 4. Desalting is ne
Page 490:
III Various Applications of Affinit
Page 494:
248 Galaev and Mattiasson in biotec
Page 498:
250 Galaev and Mattiasson 2.4. Sepa
Page 502:
252 Galaev and Mattiasson 3.4. Sepa
Page 506:
254 Galaev and Mattiasson ensure el
Page 510:
17 Monolithic Bioreactors for Macro
Page 514:
Monolithic Bioreactors for Macromol
Page 518:
Monolithic Bioreactors for Macromol
Page 522: Monolithic Bioreactors for Macromol
Page 546: 18 Plasmid DNA Purification Via the
Page 550: Plasmid DNA Purification 277 are pr
Page 554: Plasmid DNA Purification 279 5. Dec
Page 558: Plasmid DNA Purification 281 Initia
Page 562: Plasmid DNA Purification 283 8. Bou
Page 566: 286 Tchaga proteins is variable. Ho
Page 570: 288 Tchaga residue is buried and is
Page 576: Affinity Chromatography of Phosphor
Page 580: Affinity Chromatography of Phosphor
Page 584: 20 Protein Separation Using Immobil
Page 588: Immobilized Phospholipid Chromatogr
Page 600: 21 Analysis of Proteins in Solution
Page 604: Affinity Capillary Electrophoresis
Page 624:
Affinity Capillary Electrophoresis
Page 628:
Page 632:
Page 636:
Page 640:
Page 644:
Page 648:
Page 652:
Page 656:
Page 660:
Page 664:
Page 668:
Page 672:
Index Adsorption isotherm, 75, 84 A
Page 676:
Index 341 Genenase I, 170, 214, 215
Page 680:
Index 343 Saccharomyces cerevisae,
show all

View - ResearchGate

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?