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Affinity Processing of Cell-Containing Feeds 255 10. Ahlqvist, J., Kumar, A., Ledung, E., Sundström, H., Hörnsten, G. and Mattiasson, B. (2006) Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labelling with specific antibodies, J. Biotechnol. 122, 216–225. 11. Teilum, M., Hansson, M. J., Dainiak, M. B., Surve, S., Månsson, R., Elmer, E., Önnerfjord, P. and Mattiasson, G. (2006) Binding mitochondria to cryogel monoliths allow detection of proteins specifically released following calciuminduced permeability transition, Anal. Biochem. 348, 209–221. 12. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76–85. 13. Freshney, R. I., Animal Cell Culture, IRL Press, Glasgow, 1986.
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Affinity Chromatography second edit
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METHODS IN MOLECULAR BIOLOGY TM Aff
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To Tina, Emmanuella, Natalie, and A
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viii Preface Affinity tags for puri
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x Contents 10. Immobilized Metal Io
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xii Contributors Tzong-Hsien Lee
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2 Roque and Lowe cell extract conta
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4 Roque and Lowe groups critical in
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6 Roque and Lowe of reinforcement e
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8 Roque and Lowe unknown factors ar
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10 Roque and Lowe receptor domains
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12 Roque and Lowe A further issue o
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14 Roque and Lowe transgenic system
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16 Roque and Lowe 6. Arsenis, C. an
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18 Roque and Lowe 39. Burton, S.J.,
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20 Roque and Lowe 71. Bhikhabhai, R
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I Various Modes of Affinity Chromat
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26 Charlton and Zachariou Since the
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28 Charlton and Zachariou 5. Equili
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30 Charlton and Zachariou 9. Elute
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32 Charlton and Zachariou 3.3. Puri
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34 Charlton and Zachariou could als
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3 Affinity Precipitation of Protein
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Affinity Precipitation of Proteins
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4 Immunoaffinity Chromatography Stu
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Immunoaffinity Chromatography 55 2.
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Immunoaffinity Chromatography 57 A
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Immunoaffinity Chromatography 59 ab
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5 Dye Ligand Chromatography Stuart
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Dye Ligand Chromatography 63 Develo
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Dye Ligand Chromatography 65 6. Mis
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Dye Ligand Chromatography 67 Fig. 1
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Dye Ligand Chromatography 69 6. Sec
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72 Tugcu chromatography, the sorpti
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74 Tugcu non-specific interactions,
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76 Tugcu the salt counter ions on t
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78 Tugcu On a plot of log K versus
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80 Tugcu Figure 2B illustrates a ty
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82 Tugcu 2.5. Running Displacement
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84 Tugcu then the operating conditi
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86 Tugcu Table 1 Trobleshooting for
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88 Tugcu 23. Torres, A. R. and Pete
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II Affinity Chromatography Using Pu
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94 Roque and Lowe market, with 14 F
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96 Roque and Lowe and RasMol V2.7.1
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98 Roque and Lowe house using, for
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100 Roque and Lowe Gly71 and Gly72)
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102 Roque and Lowe dissolved in ace
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104 Roque and Lowe equilibration bu
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106 Roque and Lowe of color. Purple
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108 Roque and Lowe 5. Fassina, G.,
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8 Phage Display of Peptides in Liga
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Phage Display of Peptides in Ligand
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9 Preparation, Analysis and Use of
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Preparation, Analysis and Use of an
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10 Immobilized Metal Ion Affinity C
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IMAC of Histidine-Tagged Fusion Pro
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152 Godat et al. tag. HQ-tagged pro
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154 Godat et al. 2. NaCl (5 M). 3.
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156 Godat et al. 4. Invert tube to
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158 Godat et al. 3. Sonicate sample
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160 Godat et al. the above protocol
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162 Godat et al. 3.7.1. Purificatio
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164 Godat et al. 3. Adding 200 mM N
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166 Godat et al. 4. The MagneHis Ni
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168 Godat et al. 22. Lin, D., Tabb,
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170 Pattenden and Thomas 1. Introdu
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172 Pattenden and Thomas functions
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174 Pattenden and Thomas of the mal
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176 Pattenden and Thomas necessary.
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178 Pattenden and Thomas 2.4. Amylo
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180 Pattenden and Thomas 9. Thoroug
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182 Pattenden and Thomas 8. Collect
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184 Pattenden and Thomas binding ef
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186 Pattenden and Thomas 15. Cells
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188 Pattenden and Thomas 23. Martin
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13 Methods for Detection of Protein
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Detection of Protein-Protein and Pr
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212 Charlton recognition sequence,
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214 Charlton when selecting Factor
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216 Charlton 2. Materials 2.1. Reag
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218 Charlton (see Notes 12 and 13).
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220 Charlton 3.3. Cleavage of Fusio
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222 Charlton 3. The catalytic mecha
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224 Charlton may dictate. However,
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226 Charlton 22. The full-length fu
Page 458: 228 Charlton 22. Lien, S., Milner,
Page 462: 230 Arnau et al. downstream process
Page 466: 232 Arnau et al. Fig. 1. Overview o
Page 470: 234 Arnau et al. Fig. 2. The pQE-1
Page 474: 236 Arnau et al. 2.3.3. Step B Buff
Page 478: 238 Arnau et al. Fig. 3. Immobilize
Page 482: 240 Arnau et al. 6. Collect the flo
Page 486: 242 Arnau et al. 4. Desalting is ne
Page 490: III Various Applications of Affinit
Page 494: 248 Galaev and Mattiasson in biotec
Page 498: 250 Galaev and Mattiasson 2.4. Sepa
Page 502: 252 Galaev and Mattiasson 3.4. Sepa
Page 506: 254 Galaev and Mattiasson ensure el
Page 512: 258 Benčina et al. 1. Introduction
Page 516: 260 Benčina et al. 3.1.1. Static I
Page 520: 262 Benčina et al. [A] abs orbance
Page 524: 264 Benčina et al. Table 2 Long-Te
Page 528: 266 Benčina et al. Table 3 Effect
Page 532: 268 Benčina et al. 8 7 n RNase 0,0
Page 536: 270 Benčina et al. Table 6 Long-Te
Page 540: 272 Benčina et al. B A PepC marker
Page 544: 274 Benčina et al. 3. Platonova, G
Page 548: 276 Forde diseases, cancer and infe
Page 552: 278 Forde 12. Sample loading buffer
Page 556: 280 Forde 2. Mix 100 μl of sample,
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282 Forde 12. Safety Warning: Picog
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19 Affinity Chromatography of Phosp
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Affinity Chromatography of Phosphor
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20 Protein Separation Using Immobil
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Immobilized Phospholipid Chromatogr
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21 Analysis of Proteins in Solution
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Affinity Capillary Electrophoresis
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Index Adsorption isotherm, 75, 84 A
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Index 341 Genenase I, 170, 214, 215
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Index 343 Saccharomyces cerevisae,
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