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Site-Specific Cleavage of Fusion Proteins 227
7. Nagai, K. and Thøgersen, H. C. (1984). Generation of beta-globin by sequencespecific
proteolysis of a hybrid protein produced in Escherichia coli. Nature 309,
810–812.
8. Blomback, B., Blomback, M., Hessel, B. and Iwanaga, S. (1967). Structure of
N-terminal fragments of fibrinogen and specificity of thrombin. Nature 215,
1445–1448.
9. Chang, J.-Y. (1985). Thrombin specificity. Eur. J. Biochem 151, 217–224.
10. Forsberg, G., Baastrup, B., Rondahl, H. Holmgren, E., Pohl, G., Hartmanis, M.
and Lake, M. (1992). An evaluation of different enzymatic cleavage methods
for recombinant fusion proteins, applied of Des(1–3)insulin-like growth factor I.
J. Protein Chem. 11, 201–211.
11. Carter P. and Wells J. A. (1987). Engineering enzyme specificity by “substrateassisted
catalysis”. Science 237, 394–399.
12. Carter, P., Nilsson, B. Burnier, J. P., Burdick, D. and Wells, J. A. (1989).
Engineering subtilisin BPN’ for site-specific proteolysis. Proteins 6, 240–248.
13. Allison, R., Johnston, R. E. and Dougherty, W. G. (1986). The nucleotide sequence
of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis
of a single polyprotein. Virology 154, 9–20.
14. Lawson, M. A. and Semler, B. L. (1991). Poliovirus thiol proteinase 3C can utilize
a serine nucleophile within the putative catalytic triad. Proc. Natl. Acad. Sci.
U. S. A. 88, 9919–9923.
15. Carrington, J. C. and Dougherty, W. G. (1988). A viral cleavage site cassette:
identification of amino acid sequences required for tobacco etch virus polyprotein
processing. Proc. Natl. Acad. Sci. U. S. A. 85, 3391–3395.
16. Dougherty, W. G. and Parks, T. D. (1989). Molecular genetic and biochemical
evidence for the involvement of the heptapeptide cleavage sequence in determining
the reaction profile at two tobacco etch virus cleavage sites in cell-free assays.
Virology 172, 145–155.
17. Cordingley, M. G., Callahan, P. L., Sardana, V. V., Garsky, V. M. and Colonno,
R. J. (1990). Substrate requirements of human rhinovirus 3C protease for peptide
cleavage in vitro. J. Biol. Chem. 265, 9062–9065.
18. Kapust, R. B., Tozer, J., Copeland, T. D. and Waugh, D. S. (2002). The P1’
specificity of tobacco etch virus protease. Biochem. Biophys. Res. Commun. 294,
949–955.
19. Baratti, J., Maroux, S. and Louvard, D. (1973). Effect of ionic strength and calcium
ions on the activation of trypsinogen by enterokinase. Biochim. Biophys. Acta.
321, 632–638.
20. Forstner, M., Peters-Libeu, C., Contreras-Forrest, E., Newhouse, Y., Knapp, M.,
Rupp, B. and Weisgraber, K. H. (1999). Carboxyl-terminal domain of human
apolipoprotein E: expression, purification, and crystallization. Protein Expr. Purif.
17, 267–272.
21. Zhang, H., Yuan, Q., Zhu, Y. and Ma, R. (2005). Expression and preparation of
recombinant hepcidin in Escherichia coli. Protein Expr. Purif. 41, 409–416.