View - ResearchGate
View - ResearchGate View - ResearchGate
168 Godat et al. 22. Lin, D., Tabb, D.L. and Yates, J.R., III. (2003) Large-Scale Protein Identification Using Mass Spectrometry. Biochim. Biophys. Acta 1646, 1–10. 23. Yan, Z., Caldwell, G.W. and McDonell, P.A. (1999) Identification of a Gluconic Acid Derivative Attached to the N-terminus of Histidine-Tagged Proteins Expressed in Bacteria. Biochem. Biophys. Res. Commun. 262, 793–800. 24. Sauer, S., Lange, B.M.H., Gobom, J., Nyarsik, L., Seitz, H., and Lehrach, H. (2005) Miniaturization in Functional Genomics and Proteomics. Nat. Rev. Genet. 6, 465–476.
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- Page 314: 154 Godat et al. 2. NaCl (5 M). 3.
- Page 318: 156 Godat et al. 4. Invert tube to
- Page 322: 158 Godat et al. 3. Sonicate sample
- Page 326: 160 Godat et al. the above protocol
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- Page 334: 164 Godat et al. 3. Adding 200 mM N
- Page 338: 166 Godat et al. 4. The MagneHis Ni
- Page 344: 12 Amylose Affinity Chromatography
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168 Godat et al.<br />
22. Lin, D., Tabb, D.L. and Yates, J.R., III. (2003) Large-Scale Protein Identification<br />
Using Mass Spectrometry. Biochim. Biophys. Acta 1646, 1–10.<br />
23. Yan, Z., Caldwell, G.W. and McDonell, P.A. (1999) Identification of a Gluconic<br />
Acid Derivative Attached to the N-terminus of Histidine-Tagged Proteins<br />
Expressed in Bacteria. Biochem. Biophys. Res. Commun. 262, 793–800.<br />
24. Sauer, S., Lange, B.M.H., Gobom, J., Nyarsik, L., Seitz, H., and Lehrach, H.<br />
(2005) Miniaturization in Functional Genomics and Proteomics. Nat. Rev. Genet.<br />
6, 465–476.
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