View - ResearchGate
View - ResearchGate View - ResearchGate
Purification of HQ-Tagged Proteins 167 7. Porath, J., Carlsson, J., Olsson, I., and Belfrage, G. (1975) Metal Chelate Affinity Chromatography, A New Approach to Protein Fractionation. Nature 258, 598–599. 8. Lönnerdal, B. and Keen, C.L. (1982) Metal Chelate Affinity Chromatography of Proteins. J. Appl. Biochem. 4, 203–208. 9. Blommel, P.G., Martin, P.A., Wrobel, R.L., Steffen, E., and Fox, B.G. (2006) High Efficiency Single Step Production of Expression Plasmids from cDNA Clones Using the Flexi Vector Cloning System. Protein Expr. Purif. 47, 562–570. 10. Betz, N.A. (2004) Efficient Purification of His-Tagged Proteins from Insect and Mammalian Cells. Promega Notes 87, 29–32. 11. Yokoyama, S. (2003) Protein Expression Systems for Structural Genomics and Proteomics. Curr. Opin. Chem. Biol. 7, 39–43. 12. Sawasaki, T., Ogasawara, T., Morishita, R., and Endo, Y. (2002) A Cell-Free Protein Synthesis System for High-Throughput Proteomics. Proc. Natl. Acad. Sci. U. S. A. 99, 14652–14657. 13. Tabuchi, M., Hino, M., Shinohara, Y., and Baba, Y. (2002) Cell-Free Protein Synthesis on a Microchip. Proteomics 2, 430–435. 14. Cornish, V.W., Benson, D.R., Altenbach, C.A., Hideg, K., Hubbell, W.L., and Schultz, P.G. (1994) Site-Specific Incorporation of Biophysical Probes into Proteins. Proc. Natl. Acad. Sci. U. S. A. 91, 2910–2914. 15. Langland, J.O., Langland, L.A., Browning, K.S., and Roth, D.A.(1996) Phosphorylation of Plant Eukaryotic Initiation Factor-2 by the Plant Encoded Double- Stranded RNA-Dependent Protein Kinase, pPKR, and Inhibition of Protein Synthesis In Vitro. J. Biol. Chem. 271, 4539–4544. 16. Kong, A.M., Speed, C.J., O‘Malley, C.J., Layton, M.J., Meehan, T., Loveland, K.L, Cheema, S., Ooms, L.M., and Mitchell, C.A. (2000) Cloning and Characterization of a 72-kDa Inositolpolyphosphate 5-Phosphatase Localized to the Golgi Network. J. Biol. Chem. 275, 24052–24064. 17. Martin, K.H., Grosenbach, D.W., Franke, C.A., and Hruby, D.E. (1997) Identification and Analysis of Three Myristoylated Vaccinia Virus Late Proteins. J. Virol. 71, 5218–5226. 18. Lin, C.-T., Moore, P.A., Auberry, D.L., Landorf, E.V., Peppler, T., Victry, K.D., Collart, F.R., and Kery, V. (2006) Automated Purification of Recombinant Proteins: Combining High-Throughput with High Yield. Protein Expr. Purif. 47, 16–24 19. Yarmush, M.L. and Jayaraman, A. (2002) Advances in Proteomic Technologies. Ann. Rev. Biomed. Eng. 4, 349–373. 20. Hunter, T.C., Andon, N.L., Koller, A., Yates, J.R., III, and Haynes, P.A. (2002) The Functional Proteomics Toolbox: Methods and Applications. J. Chromatogr. B 782, 165–181. 21. Lim, H., Eng, J., Yates, J.R., III, Tollaksen, S.L., Giometti, C.S., Holden, J.F., Adams, M.W.W., Reich, C.I., Olsen, G.J., and Hays, L.G. (2003) Identification of 2D-Gel Proteins: A Comparison of MALDI/TOF Peptide Mass Mapping to μ LC-ESI Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom. 14, 957–970.
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Purification of HQ-Tagged Proteins 167<br />
7. Porath, J., Carlsson, J., Olsson, I., and Belfrage, G. (1975) Metal Chelate Affinity<br />
Chromatography, A New Approach to Protein Fractionation. Nature 258, 598–599.<br />
8. Lönnerdal, B. and Keen, C.L. (1982) Metal Chelate Affinity Chromatography of<br />
Proteins. J. Appl. Biochem. 4, 203–208.<br />
9. Blommel, P.G., Martin, P.A., Wrobel, R.L., Steffen, E., and Fox, B.G. (2006) High<br />
Efficiency Single Step Production of Expression Plasmids from cDNA Clones<br />
Using the Flexi Vector Cloning System. Protein Expr. Purif. 47, 562–570.<br />
10. Betz, N.A. (2004) Efficient Purification of His-Tagged Proteins from Insect and<br />
Mammalian Cells. Promega Notes 87, 29–32.<br />
11. Yokoyama, S. (2003) Protein Expression Systems for Structural Genomics and<br />
Proteomics. Curr. Opin. Chem. Biol. 7, 39–43.<br />
12. Sawasaki, T., Ogasawara, T., Morishita, R., and Endo, Y. (2002) A Cell-Free<br />
Protein Synthesis System for High-Throughput Proteomics. Proc. Natl. Acad. Sci.<br />
U. S. A. 99, 14652–14657.<br />
13. Tabuchi, M., Hino, M., Shinohara, Y., and Baba, Y. (2002) Cell-Free Protein<br />
Synthesis on a Microchip. Proteomics 2, 430–435.<br />
14. Cornish, V.W., Benson, D.R., Altenbach, C.A., Hideg, K., Hubbell, W.L., and<br />
Schultz, P.G. (1994) Site-Specific Incorporation of Biophysical Probes into<br />
Proteins. Proc. Natl. Acad. Sci. U. S. A. 91, 2910–2914.<br />
15. Langland, J.O., Langland, L.A., Browning, K.S., and Roth, D.A.(1996) Phosphorylation<br />
of Plant Eukaryotic Initiation Factor-2 by the Plant Encoded Double-<br />
Stranded RNA-Dependent Protein Kinase, pPKR, and Inhibition of Protein<br />
Synthesis In Vitro. J. Biol. Chem. 271, 4539–4544.<br />
16. Kong, A.M., Speed, C.J., O‘Malley, C.J., Layton, M.J., Meehan, T., Loveland, K.L,<br />
Cheema, S., Ooms, L.M., and Mitchell, C.A. (2000) Cloning and Characterization<br />
of a 72-kDa Inositolpolyphosphate 5-Phosphatase Localized to the Golgi Network.<br />
J. Biol. Chem. 275, 24052–24064.<br />
17. Martin, K.H., Grosenbach, D.W., Franke, C.A., and Hruby, D.E. (1997) Identification<br />
and Analysis of Three Myristoylated Vaccinia Virus Late Proteins. J. Virol.<br />
71, 5218–5226.<br />
18. Lin, C.-T., Moore, P.A., Auberry, D.L., Landorf, E.V., Peppler, T., Victry,<br />
K.D., Collart, F.R., and Kery, V. (2006) Automated Purification of Recombinant<br />
Proteins: Combining High-Throughput with High Yield. Protein Expr. Purif. 47,<br />
16–24<br />
19. Yarmush, M.L. and Jayaraman, A. (2002) Advances in Proteomic Technologies.<br />
Ann. Rev. Biomed. Eng. 4, 349–373.<br />
20. Hunter, T.C., Andon, N.L., Koller, A., Yates, J.R., III, and Haynes, P.A. (2002)<br />
The Functional Proteomics Toolbox: Methods and Applications. J. Chromatogr.<br />
B 782, 165–181.<br />
21. Lim, H., Eng, J., Yates, J.R., III, Tollaksen, S.L., Giometti, C.S., Holden, J.F.,<br />
Adams, M.W.W., Reich, C.I., Olsen, G.J., and Hays, L.G. (2003) Identification<br />
of 2D-Gel Proteins: A Comparison of MALDI/TOF Peptide Mass Mapping to μ<br />
LC-ESI Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom. 14, 957–970.