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148 Charlton and Zachariou
for some time after it has been washed out of the system (author’s personal
observations). The exclusion of such agents from the final elution may be beneficial
where the agent is refractory to the intended application of the protein target,
for example, the inclusion of detergents or chaotropes in the protein preparation.
Exclusion of these agents from the elution buffer may obviate the need for a buffer
exchange step. Likewise, incorporation of the agent prior to elution may allow
for even further reduction in the severity of the conditions determined in step 2,
Subheading 3.2.2.
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