View - ResearchGate

More documents

Recommendations

Info

144 Charlton and Zachariou Table 2 Agent Effect Comment Non-ionic detergents, e.g., Triton, Tween; No more than 10% v/v Ionic detergents, e.g., SDS (anionic), CTAB (cationic) No more than 0.5% w/v Chaotropic agents, e.g., 8 M Urea or 6 M Guanidine–HCl Organic solvent, e.g., Isopropanol No more than 20% v/v pH > 9 pH
Page 2:
Affinity Chromatography second edit
Page 6:
METHODS IN MOLECULAR BIOLOGY TM Aff
Page 10:
To Tina, Emmanuella, Natalie, and A
Page 14:
viii Preface Affinity tags for puri
Page 18:
x Contents 10. Immobilized Metal Io
Page 22:
xii Contributors Tzong-Hsien Lee
Page 26:
2 Roque and Lowe cell extract conta
Page 30:
4 Roque and Lowe groups critical in
Page 34:
6 Roque and Lowe of reinforcement e
Page 38:
8 Roque and Lowe unknown factors ar
Page 42:
10 Roque and Lowe receptor domains
Page 46:
12 Roque and Lowe A further issue o
Page 50:
14 Roque and Lowe transgenic system
Page 54:
16 Roque and Lowe 6. Arsenis, C. an
Page 58:
18 Roque and Lowe 39. Burton, S.J.,
Page 62:
20 Roque and Lowe 71. Bhikhabhai, R
Page 66:
I Various Modes of Affinity Chromat
Page 70:
26 Charlton and Zachariou Since the
Page 74:
28 Charlton and Zachariou 5. Equili
Page 78:
30 Charlton and Zachariou 9. Elute
Page 82:
32 Charlton and Zachariou 3.3. Puri
Page 86:
34 Charlton and Zachariou could als
Page 90:
3 Affinity Precipitation of Protein
Page 94:
Affinity Precipitation of Proteins
Page 98:
Page 102:
Page 106:
Page 110:
Page 114:
Page 118:
Page 122:
4 Immunoaffinity Chromatography Stu
Page 126:
Immunoaffinity Chromatography 55 2.
Page 130:
Immunoaffinity Chromatography 57 A
Page 134:
Immunoaffinity Chromatography 59 ab
Page 138:
5 Dye Ligand Chromatography Stuart
Page 142:
Dye Ligand Chromatography 63 Develo
Page 146:
Dye Ligand Chromatography 65 6. Mis
Page 150:
Dye Ligand Chromatography 67 Fig. 1
Page 154:
Dye Ligand Chromatography 69 6. Sec
Page 158:
72 Tugcu chromatography, the sorpti
Page 162:
74 Tugcu non-specific interactions,
Page 166:
76 Tugcu the salt counter ions on t
Page 170:
78 Tugcu On a plot of log K versus
Page 174:
80 Tugcu Figure 2B illustrates a ty
Page 178:
82 Tugcu 2.5. Running Displacement
Page 182:
84 Tugcu then the operating conditi
Page 186:
86 Tugcu Table 1 Trobleshooting for
Page 190:
88 Tugcu 23. Torres, A. R. and Pete
Page 194:
II Affinity Chromatography Using Pu
Page 198:
94 Roque and Lowe market, with 14 F
Page 202:
96 Roque and Lowe and RasMol V2.7.1
Page 206:
98 Roque and Lowe house using, for
Page 210:
100 Roque and Lowe Gly71 and Gly72)
Page 214:
102 Roque and Lowe dissolved in ace
Page 218:
104 Roque and Lowe equilibration bu
Page 222:
106 Roque and Lowe of color. Purple
Page 226:
108 Roque and Lowe 5. Fassina, G.,
Page 230:
8 Phage Display of Peptides in Liga
Page 234:
Phage Display of Peptides in Ligand
Page 238:
Page 242:
Page 246: Phage Display of Peptides in Ligand
Page 258: 9 Preparation, Analysis and Use of
Page 262: Preparation, Analysis and Use of an
Page 282: 10 Immobilized Metal Ion Affinity C
Page 286: IMAC of Histidine-Tagged Fusion Pro
Page 300: 146 Charlton and Zachariou the Tabl
Page 304: 148 Charlton and Zachariou for some
Page 308: 11 Methods for the Purification of
Page 312: Purification of HQ-Tagged Proteins
Page 344: 12 Amylose Affinity Chromatography
Page 348:
Amylose Affinity Chromatography of
Page 352:
Page 356:
Page 360:
Page 364:
Page 368:
Page 372:
Page 376:
Page 380:
Page 384:
Page 388:
192 Urh et al. and affinity purific
Page 392:
194 Urh et al. beads with HaloTag l
Page 396:
196 Urh et al. 2.3. Detection of Pr
Page 400:
198 Urh et al. 2. Carefully remove
Page 404:
200 Urh et al. 3.2.1.2. Phase 2 Imm
Page 408:
202 Urh et al. 3.2.2. Detection of
Page 412:
204 Urh et al. The following protoc
Page 416:
206 Urh et al. 3. Incubate for 10 m
Page 420:
208 Urh et al. by 0.5% Triton X-100
Page 424:
14 Site-Specific Cleavage of Fusion
Page 428:
Site-Specific Cleavage of Fusion Pr
Page 432:
Page 436:
Page 440:
Page 444:
Page 448:
Page 452:
Page 456:
Page 460:
15 The Use of TAGZyme for the Effic
Page 464:
Removal of N-Terminal His-Tags 231
Page 468:
Page 472:
Page 476:
Page 480:
Page 484:
Page 488:
Page 492:
16 Affinity Processing of Cell-Cont
Page 496:
Affinity Processing of Cell-Contain
Page 500:
Page 504:
Page 508:
Page 512:
258 Benčina et al. 1. Introduction
Page 516:
260 Benčina et al. 3.1.1. Static I
Page 520:
262 Benčina et al. [A] abs orbance
Page 524:
264 Benčina et al. Table 2 Long-Te
Page 528:
266 Benčina et al. Table 3 Effect
Page 532:
268 Benčina et al. 8 7 n RNase 0,0
Page 536:
270 Benčina et al. Table 6 Long-Te
Page 540:
272 Benčina et al. B A PepC marker
Page 544:
274 Benčina et al. 3. Platonova, G
Page 548:
276 Forde diseases, cancer and infe
Page 552:
278 Forde 12. Sample loading buffer
Page 556:
280 Forde 2. Mix 100 μl of sample,
Page 560:
282 Forde 12. Safety Warning: Picog
Page 564:
19 Affinity Chromatography of Phosp
Page 568:
Affinity Chromatography of Phosphor
Page 572:
Page 576:
Page 580:
Page 584:
20 Protein Separation Using Immobil
Page 588:
Immobilized Phospholipid Chromatogr
Page 592:
Page 596:
Page 600:
21 Analysis of Proteins in Solution
Page 604:
Affinity Capillary Electrophoresis
Page 608:
Page 612:
Page 616:
Page 620:
Page 624:
Page 628:
Page 632:
Page 636:
Page 640:
Page 644:
Page 648:
Page 652:
Page 656:
Page 660:
Page 664:
Page 668:
Page 672:
Index Adsorption isotherm, 75, 84 A
Page 676:
Index 341 Genenase I, 170, 214, 215
Page 680:
Index 343 Saccharomyces cerevisae,
show all

View - ResearchGate

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?