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78 Tugcu
On a plot of log K versus (dynamic affinity plot, see Fig. 2A), Eq. 9
defines two regions separated by a line with a slope of log() and an intercept
of log(). The line intercepts the point log() on the y-axis and passes through
the point defined by the parameters K a and a of component “a” (determined as
described previously). Because this plot gives information regarding the elution
order of the components in a displacement train (increasing dynamic affinity in
the upwards direction), it may also be used to compare displacer efficacies for
separating a protein mixture under specific salt and displacer concentrations.
Figure 2A illustrates a dynamic affinity plot for three solutes “A,” “B”
and “C” at a value of 10. Under the experimental conditions specified by the
100
A with higher dynamic affinity
than B
K
10
1
Δ
C with lower dynamic affinity than B
B
Increasing dynamic affinity
0.1
0 2 4 6 8 10
ν
10
λ (dynamic affinity)
1
A
C
B
0.1
1 10 100
Δ (displacer partition ratio)
Fig. 2. (Continued).