Sample Submission Forms

HARVARD MICROCHEMISTRY
16 Divinity Avenue Cambridge MA 02138-2020
617-495-4043 FAX 617-495-1374
TO:
FAX:
FROM: Liam McCallum, Laboratory Coordinator
PAGES (including this sheet): 5
1. Please do not prepare, run gels or send samples without first discussing the project and prep
procedures with our director, Bill Lane. Optimum protocols can change frequently---one of the most
significant determinants of the success of your analysis will be the preparation.
2. We ask for your patience since Bill Lane’s phone queue is usually 10 – 20 people at any point.
Although it will usually take between 2 and 5 days to return a call, your call has been logged, and Bill will
return it as soon as he reaches your point in the queue. We can appreciate that this requires your patience, but
it is also the fairest to researchers who called before you. We believe strongly that a fully reviewed project
and preparation procedure ultimately leads to the most expedient and sensitive analyses.
3. Please make copies of the attached forms and save your originals.
4. Enclosed is a form for each of the services offered at the Harvard Microchemistry Facility. Each sample
must be accompanied by a completed form. The information you provide on these forms helps to ensure
proper handling of your sample. Please include both a purchase order number and the purchase order
amount---samples submitted with incomplete billing information (i.e., no PO number) cannot be
accepted. Analysis of your sample will be delayed until complete billing information is received.
5. If you are submitting a sample for protein identification, only the DIGESTION/SEPARATION FORM should
be completed. We track the subsequent LC/MS/MS and/or sequencing process from this form. You should not
send a MASS SPEC or PROTEIN SEQUENCE FORM. The latter are only used for individual MW
determinations or intact N-terminal sequence determinations, respectively.
6. When requesting an amino acid analysis to quantitate your sample for N-terminal sequencing, you should
include with your PROTEIN SEQUENCE ANALYSIS FORM(S) a completed AMINO ACID ANALYSIS
FORM specifying the % for AAA and the % for sequence.
7. Important information to include on your forms:
• Sample Name (which should match the name on the sample tube).
• Your phone number and fax number.
• Your billing information: PO number, billing address, and purchasing agent phone number; please arrange to
send a hard copy of your PO when ready, either with your sample or by fax.
• The MW, quantitation (even if only an estimate), and method of quantitation.
• The organism or species (not the vector, please) from which your polypeptide is derived.
• For direct sequencing (not digests), be sure to specify the number of sequencing cycles desired.
• Note: Radiolabelled samples must be cleared with Bill Lane prior to submission.
It is best to send samples via FedEx as there is no parking in our immediate vicinity; if you do choose to drop off
samples in person please be advised that drop-off hours are Monday through Friday, 10:00 AM to 3:00 PM.
Qualified personnel are not available outside those hours. If you have any questions, please call us.
REMEMBER: All fields in the submitted forms must be completed!
INCOMPLETE or INCORRECT FORMS WILL DELAY YOUR SAMPLE!

DIGESTION/SEPARATION
HARVARD MICROCHEMISTRY FACILITY
SAMPLE NAME: __________________________________
(This should match tube)
YOUR NAME: ____________________________________
DATE SUBMITTED: ___________________________
PHONE(s): ___________________________
FAX: ___________________________
‣ Billing and Sample Information Must Be Completed Before Submission Of The Sample. Please Do Not Leave Any
Information Blank. Indicate estimated values with a tilde (~) or a range (e.g. 5Kd - 15Kd or ~80 µl). Samples should be
provided salt, buffer and detergent free. Any other conditions should be discussed before submitting sample.
Billing Information:
PRINCIPAL INVESTIGATOR:
Instititution:
Billing Address:
Purchasing Agent Phone #:
Harvard/Affiliate's 33-digit Account #: _ _ _ - _ _ _ _ _ - _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ - _ _ _ _ _
Non-Harvard User's P.O. Number:
MOLECULAR WEIGHT: kD ESTIMATED WEIGHT: µg
VOLUME: µl % ACRYLAMIDE:__ BUFFER: ESTIMATED AMOUNT: pmol
ORGANISM, TISSUE SOURCE:
ESTIMATE METHOD:
PVDF: NC: Gel*: * Solution: TOTAL SURFACE AREA: mm²
*If sample is in gel, what stain was used ____CBB____silver stain____copper stain____sypro red. Brand ____________
‣ Please comment on the purpose of the analysis and review any special instructions. N.B. In the absence of any
instructions below this sample will be digested with trypsin or lysylendopeptidase and peptides separated by microbore
HPLC.
‣ Radiolabelled samples are restricted to less than 2000 DPM of 3 H or 14 C. Such samples will not be accepted without
prior clearance with the facility, proper yellow radiolabel tape, on the tube and a printout of the measured DPM.
(For Lab Use Only)
Observed Staining __________ Other Noted Characteristics __________________________________________________
Sample Volume or Surface Area Used ___________µl mm²
Percent of Original Sample Used __________%
Digestion Volume ________µl Buffer __________________ Enzyme ____________ E/S Ratio ________
Alkylation _________________ Digestion Time __________h
Ready Volume ______µl Ready Date ____________
William S. Lane 16 Divinity Avenue Cambridge MA 02138 (617) 495-4043 FAX (617) 495-1374
(5/20/2003 Digestion Separation Form.doc)

PROTEIN SEQUENCE ANALYSIS
HARVARD MICROCHEMISTRY FACILITY
SAMPLE NAME: __________________________________
(This should match tube)
YOUR NAME: ____________________________________
DATE SUBMITTED: ___________________________
PHONE(s): ___________________________
FAX: ___________________________
Billing Information:
PRINCIPAL INVESTIGATOR:
Instititution:
Billing Address:
Purchasing Agent Phone #:
Harvard/Affiliate's 33-digit Account #: _ _ _ - _ _ _ _ _ - _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ - _ _ _ _ _
Non-Harvard User's P.O. Number:
‣ Billing and Sample Information must be completed before submission of the sample. Samples should be provided salt,
buffer and detergent free. Any other conditions should be discussed before submitting sample. This form is to be used for
direct sequencing of samples only and not for samples which are to be digested/separated by the facility prior to
sequencing. Unless otherwise discussed, 10% of the sample is routinely taken for quantitation by amino acid analysis
prior to protein sequencing. Please complete an amino acid analysis form to accompany this form.
‣ Please complete all fields, blank Information on this form will delay analysis of your sample!,
MOLECULAR WEIGHT: Kd ESTIMATED WEIGHT: µg
VOLUME: µl BUFFER: ESTIMATED AMOUNT: pmol
ORGANISM, TISSUE SOURCE:
ESTIMATED BY:
BLOTS ONLY: PVDF BRAND, NAME: TOTAL SURFACE AREA: mm²
‣ NUMBER OF CYCLES TO RUN: Please indicate ONE of the following:
Cycles exactly
5 cycles only (minimum)
As many as possible*, specify maximum:
*Note: charge is per cycle run, not data obtained
‣ Please comment on the purpose of the sequencing and review any special instructions. N.B.: In the absence of any
instructions, 100% of the sample remaining after quantitation by AAA will be subjected to direct amino terminal sequencing.
‣ Radiolabelled samples are restricted to less than 2000 DPM of 3 H or 14 C. Such samples will not be accepted without
prior clearance with the facility, proper yellow radiolabel tape on the tube, and a printout of the measured DPM.
Isotope: __________________
DPM: ___________________
William S. Lane 16 Divinity Avenue Cambridge MA 02138 (617) 495-4043 FAX (617) 495-1374
(5/20/2003 Sequence Analysis Form.doc)

MASS SPECTROMETRIC ANALYSIS
HARVARD MICROCHEMISTRY FACILITY
DATE SUBMITTED: ______________________
YOUR NAME: ___________________________
PHONE: _____________________
FAX: _____________________
Billing Information:
PRINCIPAL INVESTIGATOR:
Instititution:
Billing Address:
Purchasing Agent Phone #:
Harvard/Affiliate's 33-digit Account #: _ _ _ - _ _ _ _ _ - _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ - _ _ _ _ _
Non-Harvard User's P.O. Number:
‣ Billing and Sample Information must be completed before submission of the sample. Incomplete forms will delay your
analysis. Samples should be provided salt, buffer and detergent free. Any other conditions should be discussed.
‣ Note: a 10% aliquot will be taken for amino acid analysis, unless otherwise discussed with W. Lane. Radiolabeled
samples are not accepted without prior permission, and should be properly labeled on this form and on the sample tube.
‣ Please comment on the purpose of the analysis, (e.g. confirmation of known MW, screening of a protein digest,
synthetic peptide purity, post-translational modification, screening for heterogeneity, etc.):
1
ESI-MS or
MALDI-MS
Sample Name
(on tube)
MW (known or
estimate)
Weight
µg
Amt
pmol
Vol
µl
Organism and Tissue Source,
Solvent, Comments
2
3
4
5
6
7
8
William S. Lane 16 Divinity Avenue Cambridge MA 02138 (617) 495-4043 FAX (617) 495-1374
(2/21/02 Mass Spec Analysis Form.doc)

AMINO ACID ANALYSIS
HARVARD MICROCHEMISTRY FACILITY
DATE SUBMITTED: ______________________
YOUR NAME: ___________________________
PHONE: _____________________
FAX: _____________________
Billing Information:
PRINCIPAL INVESTIGATOR:
Instititution:
Billing Address:
Purchasing Agent Phone #:
Harvard/Affiliate's 33-digit Account #: _ _ _ - _ _ _ _ _ - _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ - _ _ _ _ _
Non-Harvard User's P.O. Number:
Billing and sample information must be completed before submission of the sample.
Samples should be provided free of buffers, salts and detergents. Any other conditions should be discussed. Blotted samples can
be analyzed on PVDF, but not on nitrocellulose membrane. *In the absence of other instructions a 10% aliquot will be taken for
amino acid analysis. Radiolabeled samples are not accepted without prior permission, and should be properly labeled on this form
and on the sample tube.
Name of organism sample is derived from __________________________________________________
Please list any further instructions or comments:
Purpose of Analysis:
Quantitation (100 ng minimum)
Composition (100 pmol minimum)
Other _________________________
1
Sample Name
(on tube)
MW (minus
nonpeptidic
component)
~No.
of
AA's
Wt
µg
Amt
pmol
Vol
µl
%
for
AAA*
*Sequence if known*
also: Misc. notes, Organism and
Tissue Source, Solvent, Comments,
2
3
4
5
6
7
8
William S. Lane 16 Divinity Avenue Cambridge MA 02138 (617) 495-4043 FAX (617) 495-1374
(5/20/2003 Amino Acid Analysis Form.doc)