Introduction to Enzyme and Coenzyme Chemistry - E-Library Home

258 Chapter 12
Figure 12.2 Structure of hairpin ribozyme, complexed with vanadate transition state analogue
(PDB Wle 1M5O). The structure consists of two ribonucleotides: chain A (in black), which is the
substrate for phosphodiester cleavage; and RNA chain B (in red), which is the catalytic chain. The
vanadate transition state analogue, incorporated in chain A, is shown in bold.
N
N
A 9
N
A 9
N
A 38
N
N
N
H
N
N
O
H
N
N
A
O N
H H
CH
O O 3 O
P
O H
N
O −
O
H
N N
G
O
N
N
N
G 8
A 38
N
N
N
H
N
N
O
H
H
N
N
A
O N
H H
O
O O
− O
H
V
N
− O
O
H N N
G
O
N
N
N
G 8
O
OH
O
OH
(a) Interactions in ground state
(b) Interactions in transition state
Figure 12.3 Transition state stabilisation in hairpin ribozyme.