WHO monographs on selected medicinal plants - travolekar.ru

Ramulus cum Uncis Uncariae
scavenging activity against superoxide anion radicals in vitro, as measured
by electron spin resonance spin-trapping techniques (36).
Depressant effects on the central nervous system
Intragastric administration of an aqueous extract of the crude drug (2.0 g/
kg bw), or oxindole alkaloids isolated from the crude drug (10–100 mg/kg
bw) to mice, significantly prolonged thiopental-induced sleep time
(p < 0.05) (13, 37). Intragastric administration of an aqueous extract of the
crude drug to mice at a dose of 2.0 g/kg bw significantly depressed locomotor
activity (p < 0.05) (37). Intragastric administration of three indole
alkaloids isolated from the crude drug, corynoxine (30.0 mg/kg bw),
corynoxine B (100.0 mg/kg bw) or isorhynchophylline (100.0 mg/kg bw)
also significantly decreased locomotor activity (p < 0.05) (38).
The suppressant effects on the central nervous system were confirmed
by a study in mice of extracts prepared from mixtures of the herb with
and without the crude drug. Preparations containing the crude drug prolonged
thiopental-induced sleep, but the extract prepared from the mixture
of herb without the crude drug was devoid of this activity (39). Intraperitoneal
administration of a 90% ethanol extract of the crude drug
reduced spontaneous motor activity in mice (34).
Effects on neurotransmitters
An 80% ethanol extract of the crude drug inhibited serotonin reuptake
by 97% at a concentration of 10.0 μg/ml in rat brain stem neurons (40).
Uncarinic acids A and B, isolated from the crude drug inhibited the activity
of phosphatidylinositol-specific phospholipase C, a key enzyme involved
in the signal transduction of growth factors, neurotransmitters
and hormones, with a median inhibitory concentration of 35.66 and
44.55 μM, respectively (41). Hirsutine, at a concentration of 300 nM to
10.0 μM inhibited dopamine release induced by nicotine in rat pheochromocytoma
PC12 cells (42). In a study in rats, dopamine release induced
by potassium chloride was also inhibited by hirsutine at a concentration
of 10.0 μM (41). Hirsutine also had antagonistic effects on the opioid receptors
and, at a concentration of 10.0 μM, reversed the inhibitory effect
of morphine on twitch contraction in isolated guinea-pig ileum (43).
Toxicology
Intraperitoneal administration of a 90% ethanol extract of the crude drug
to mice had a median lethal dose of 1.0 g/kg bw (34).
Clinical pharmacology
No information was found.
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