WHO monographs on selected medicinal plants - travolekar.ru

<strong>WHO</strong> <strong>monographs</strong> on selected medicinal plants
the liver was also increased in animals treated with the rhizome extract.
Intragastric administration of a methanol extract of the crude drug to mice,
at a dose of 670.0 mg/kg bw, suppressed carbon tetrachloride-induced
hepatotoxicity (26).
Induction of liver injury in 16 mice by injection of carbon tetrachloride
(CCl 4
) for 9 weeks was reduced by the rhizome. Eight animals were
fed the crude drug extract (12.0 mg/kg bw) daily, for 10 days before CCl 4
injection. Control mice (n = 6) were injected with olive oil for the same
period. Serum markers of liver injury and histology of liver tissues were
studied. Hepatic glutathione, total thiol, glucose-6-phosphate dehydrogenase,
catalase, lipid peroxidation and plasma membrane-bound Na + /K +
ATPase were also assessed. Feeding the crude drug extract to CCl 4
-treated
mice reduced serum alanine aminotransferase, aspartate aminotransferase,
liver glutathione, catalase and membrane-bound Na + /K + ATPase.
Histological lesions of liver and lipid peroxidation were also significantly
fewer in these animals (27).
Intragastric administration of a dried 75% methanol extract of the
powdered rhizomes, at a dose of 150.0 mg/kg bw inhibited the induction
of N-nitrosodiethylamine-induced hepatocarcinogenesis and prevented
lipid peroxide formation as well as reducing the levels of aniline hydrase
and -glutamyl transpeptidase (28). Intragastric administration of a 95%
ethanol extract of the crude drug at a dose of 12.0–25.0 mg/kg bw for
7 days reduced galactosamine and monocrotaline-induced hepatotoxicity
(29, 30). The effect of an iridoid glycoside containing an extract of the rhizome
on the carcinogenic response and on hepatic and renal antioxidant
enzymes of rats treated with 1,2-dimethylhydrazine hydrochloride was
investigated (15). 1,2-dimethylhydrazine hydrochloride-induced hepatic
carcinogenic response and necrosis were inhibited by oral administration
of the extract at doses of 40.0 and 200.0 mg/kg bw. Liver -glutamyl transpeptidase
was reduced to 0.22 ± 0.04 and 0.18 ± 0.03 nmol/mg protein,
respectively, by the treatment. Depletion of hepatic and renal antioxidant
enzymes such as catalase and superoxide dismutase levels induced by 1,2-
dimethylhydrazine hydrochloride was reversed by the treatment, and elevated
lipid peroxidation in liver, kidney and serum was reduced. Renal
glutathione S-transferase and hepatic glutathione levels which had been
depleted by 1,2-dimethylhydrazine hydrochloride were also increased.
Oral administration of a standardized iridoid glycoside fraction of the
crude drug (25.0 mg/kg bw daily for 15 days), significantly reduced the
increases in the activities of tau-glutamyl transpeptidase, 5´-nucleotidase,
acid phosphatase and acid ribonuclease, and decreased the activities of succinate
dehydrogenase and glucose-6-phosphatase in liver, and the level of
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