WHO monographs on selected medicinal plants - travolekar.ru

Fructus Macrocarponii
of 240 ml of commercial cranberry juice cocktail (30% pure juice), and in
cranberry proanthocyanidin extract (pH 6.5) (2-fold dilution series).
These bacteria were then harvested and screened for ability to adhere to
isolated uroepithelial cells and to agglutinate human red blood cells (A1,
Rh+), and resin beads coated with isolated P-receptor oligosaccharides.
Urine collected from healthy women after consumption of the cranberry
juice cocktail (average pH 6.2) prevented adhesion in 31 (80%) of the
39 isolates and 19 (79%) of the 24 antibiotic-resistant isolates in all bioassays,
while urine collected prior to the administration of the juice (average
pH 6.2) failed to prevent adhesion in any of the samples. Anti-adhesion
activity was evident in the urine within 2 hours and persisted for up to
10 hours following ingestion of the cranberry juice cocktail. The extracted
proanthocyanidins inhibited adhesion of all isolates at concentrations
ranging from 6 to 375.0 μg/ml, demonstrating potent in vitro anti-adhesion
activity against these bacterial strains (32).
In another study, urine samples were collected from groups of volunteers
following the consumption of water, ascorbic acid or cranberry supplements
(unspecified) and tested in an anti-adhesion assay. Only intake of ascorbic
acid consistently produced acidic urine. Surface tension measurements of
the urine collected showed that both water and cranberry supplementation
consistently produced urine with a surface tension higher than that in urine
from the control group or urine collected following ascorbic acid intake.
Urine obtained after supplementation with ascorbic acid or cranberry reduced
the initial in vitro deposition rates and numbers of adherent E. coli
and Enterococcus faecalis, but not Pseudomonas aeruginosa, Staphylococcus
epidermidis, or Candida albicans. Conversely, urine obtained from subjects
with increased water intake vastly increased the initial deposition rates and
numbers of adherent E. coli and E. faecalis (p < 0.05) (33).
Antioxidant activity
The antioxidant activities of the fruit and its phenolic constituents were
measured in vitro. The fruit had an anthocyanin concentration of 0.32 mg/g
fresh weight and a total phenolic concentration of 3.15 mg/g fresh weight.
In vitro the fruit exhibited antioxidant effects at a concentration of
18.5 μmol/g fresh weight. Chlorogenic acid, peonidin 3-galactoside, cyanidin
3-galactoside and cyanidin 3-galactoside were the most important
antioxidants in the fruit (34). Methanol extracts of the fruit were assayed
for radical-scavenging activity and cell growth inhibition using seven tumour
cell lines. A methanol extract of the fruit caused inhibition of the
proliferation of K562 and HT-29 cell lines at concentrations in the range
of 16.0–125.0 μg/ml. Radical-scavenging activity was greatest in an extract
composed primarily of flavonol glycosides. Seven flavonol mono-
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