WHO monographs on selected medicinal plants - travolekar.ru

WHO monographs on selected medicinal plants - travolekar.ru WHO monographs on selected medicinal plants - travolekar.ru

20.01.2015 Views

ong>WHOong> ong>monographsong> on selected medicinal plants Plant material of interest: dried ripe fruit General appearance The dried berry is dark red to almost black, 7.0–11.5 mm in width and 10–15 mm in length, with a smooth but deeply wrinkled, slightly lustrous surface. At the fruit apex is the dried, slightly raised nectariferous disc, 1.5–2 mm across, with a shallow depression in its centre inside which is a small protuberance from the remains of the style. At the fruit base is a small scar where the berry was attached to the pedicel. The texture is spongy. In cross-section, the mesocarp and endocarp are a dull red. The mesocarp has large air pockets, and varies in thickness when dried from 0.1–2 mm. In cross-section, the fruit is divided into 4 chambers (carpels), each running the entire length of the fruit. The chambers are separated by dull red, thin, translucent septa and each chamber contains 1–5 seeds. Each seed is narrowly ovoid with an acute apex, 1–2.7 mm long and approximately 1 mm wide, with a lustrous rose to red testa that is longitudinally wrinkled. The endosperm is an opaque white (1). Organoleptic properties Odour: slightly fruity and sweet; taste: very acidic and tart (1). Microscopic characteristics Fruit: the exocarp consists of anthocyanin-coloured polygonal cells covered by a thick cuticle. Groups of cells are separated by fairly thick, colourless walls, whereas the walls within the groups are very thin. The mesocarp consists of large, spherical, thin-walled cells in which small bundles of spirally thickened vessels are embedded. Seed (cross-section): the epidermis of the testa is composed of radially elongated rectangular cells filled with mucilage. The walls are thickened in a U-shape, the thickest wall being the exterior one; the mucilage is radially striated, and in the centre of the cell is a small lumen. Below the epidermis are several layers of polygonal cells with thick, brown, occasionally reticulately thickened walls. These cells are 250–350 m long and approximately 80 m broad. The innermost layer of the testa consists of compressed, rectangular cells with sinuous longitudinal walls. The voluminous endosperm is made up of small polygonal and oil-containing cells (1). Powdered plant material Numerous fragments of the exocarp with colourless cell walls and violet contents; thin-walled parenchyma of the mesocarp; thickened and pitted cells of the testa; oil droplets (1). 150

Fructus Macrocarponii General identity tests Macroscopic and microscopic examinations and high-performance thinlayer chromatography (1). Purity tests Microbiological Tests for specific microorganisms and microbial contamination limits are as described in the ong>WHOong> guidelines on assessing quality of herbal medicines with reference to contaminants and residues (5). Foreign organic matter To be established in accordance with national requirements. Total ash To be established in accordance with national requirements. Acid-insoluble ash To be established in accordance with national requirements. Water-soluble extractive To be established in accordance with national requirements. Alcohol-soluble extractive To be established in accordance with national requirements. Loss on drying To be established in accordance with national requirements. Pesticide residues The recommended maximum limit of aldrin and dieldrin is not more than 0.05 mg/kg (6). For other pesticides, see the European pharmacopoeia (6) and the ong>WHOong> guidelines on assessing quality of herbal medicines with reference to contaminants and residues (5) and pesticide residues (7). Heavy metals For maximum limits and analysis of heavy metals, consult the ong>WHOong> guidelines on assessing quality of herbal medicines with reference to contaminants and residues (5). Radioactive residues Where applicable, consult the ong>WHOong> guidelines on assessing quality of herbal medicines with reference to contaminants and residues (5). 151

<str<strong>on</strong>g>WHO</str<strong>on</strong>g> <str<strong>on</strong>g>m<strong>on</strong>ographs</str<strong>on</strong>g> <strong>on</strong> <strong>selected</strong> <strong>medicinal</strong> <strong>plants</strong><br />

Plant material of interest: dried ripe f<strong>ru</strong>it<br />

General appearance<br />

The dried berry is dark red to almost black, 7.0–11.5 mm in width and<br />

10–15 mm in length, with a smooth but deeply wrinkled, slightly lustrous<br />

surface. At the f<strong>ru</strong>it apex is the dried, slightly raised nectariferous disc,<br />

1.5–2 mm across, with a shallow depressi<strong>on</strong> in its centre inside which is a<br />

small protuberance from the remains of the style. At the f<strong>ru</strong>it base is a<br />

small scar where the berry was attached to the pedicel. The texture is<br />

sp<strong>on</strong>gy. In cross-secti<strong>on</strong>, the mesocarp and endocarp are a dull red. The<br />

mesocarp has large air pockets, and varies in thickness when dried from<br />

0.1–2 mm. In cross-secti<strong>on</strong>, the f<strong>ru</strong>it is divided into 4 chambers (carpels),<br />

each <strong>ru</strong>nning the entire length of the f<strong>ru</strong>it. The chambers are separated by<br />

dull red, thin, translucent septa and each chamber c<strong>on</strong>tains 1–5 seeds.<br />

Each seed is narrowly ovoid with an acute apex, 1–2.7 mm l<strong>on</strong>g and approximately<br />

1 mm wide, with a lustrous rose to red testa that is l<strong>on</strong>gitudinally<br />

wrinkled. The endosperm is an opaque white (1).<br />

Organoleptic properties<br />

Odour: slightly f<strong>ru</strong>ity and sweet; taste: very acidic and tart (1).<br />

Microscopic characteristics<br />

F<strong>ru</strong>it: the exocarp c<strong>on</strong>sists of anthocyanin-coloured polyg<strong>on</strong>al cells covered<br />

by a thick cuticle. Groups of cells are separated by fairly thick,<br />

colourless walls, whereas the walls within the groups are very thin. The<br />

mesocarp c<strong>on</strong>sists of large, spherical, thin-walled cells in which small<br />

bundles of spirally thickened vessels are embedded.<br />

Seed (cross-secti<strong>on</strong>): the epidermis of the testa is composed of radially<br />

el<strong>on</strong>gated rectangular cells filled with mucilage. The walls are thickened in<br />

a U-shape, the thickest wall being the exterior <strong>on</strong>e; the mucilage is radially<br />

striated, and in the centre of the cell is a small lumen. Below the epidermis<br />

are several layers of polyg<strong>on</strong>al cells with thick, brown, occasi<strong>on</strong>ally reticulately<br />

thickened walls. These cells are 250–350 m l<strong>on</strong>g and approximately<br />

80 m broad. The innermost layer of the testa c<strong>on</strong>sists of compressed, rectangular<br />

cells with sinuous l<strong>on</strong>gitudinal walls. The voluminous endosperm<br />

is made up of small polyg<strong>on</strong>al and oil-c<strong>on</strong>taining cells (1).<br />

Powdered plant material<br />

Numerous fragments of the exocarp with colourless cell walls and violet<br />

c<strong>on</strong>tents; thin-walled parenchyma of the mesocarp; thickened and pitted<br />

cells of the testa; oil droplets (1).<br />

150

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