WHO monographs on selected medicinal plants - travolekar.ru
WHO monographs on selected medicinal plants - travolekar.ru WHO monographs on selected medicinal plants - travolekar.ru
Fructus Macrocarponii General identity tests Macroscopic and microscopic examinations and high-performance thinlayer chromatography (1). Purity tests Microbiological Tests for specific microorganisms and microbial contamination limits are as described in the
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<str<strong>on</strong>g>WHO</str<strong>on</strong>g> <str<strong>on</strong>g>m<strong>on</strong>ographs</str<strong>on</strong>g> <strong>on</strong> <strong>selected</strong> <strong>medicinal</strong> <strong>plants</strong><br />
Plant material of interest: dried ripe f<strong>ru</strong>it<br />
General appearance<br />
The dried berry is dark red to almost black, 7.0–11.5 mm in width and<br />
10–15 mm in length, with a smooth but deeply wrinkled, slightly lustrous<br />
surface. At the f<strong>ru</strong>it apex is the dried, slightly raised nectariferous disc,<br />
1.5–2 mm across, with a shallow depressi<strong>on</strong> in its centre inside which is a<br />
small protuberance from the remains of the style. At the f<strong>ru</strong>it base is a<br />
small scar where the berry was attached to the pedicel. The texture is<br />
sp<strong>on</strong>gy. In cross-secti<strong>on</strong>, the mesocarp and endocarp are a dull red. The<br />
mesocarp has large air pockets, and varies in thickness when dried from<br />
0.1–2 mm. In cross-secti<strong>on</strong>, the f<strong>ru</strong>it is divided into 4 chambers (carpels),<br />
each <strong>ru</strong>nning the entire length of the f<strong>ru</strong>it. The chambers are separated by<br />
dull red, thin, translucent septa and each chamber c<strong>on</strong>tains 1–5 seeds.<br />
Each seed is narrowly ovoid with an acute apex, 1–2.7 mm l<strong>on</strong>g and approximately<br />
1 mm wide, with a lustrous rose to red testa that is l<strong>on</strong>gitudinally<br />
wrinkled. The endosperm is an opaque white (1).<br />
Organoleptic properties<br />
Odour: slightly f<strong>ru</strong>ity and sweet; taste: very acidic and tart (1).<br />
Microscopic characteristics<br />
F<strong>ru</strong>it: the exocarp c<strong>on</strong>sists of anthocyanin-coloured polyg<strong>on</strong>al cells covered<br />
by a thick cuticle. Groups of cells are separated by fairly thick,<br />
colourless walls, whereas the walls within the groups are very thin. The<br />
mesocarp c<strong>on</strong>sists of large, spherical, thin-walled cells in which small<br />
bundles of spirally thickened vessels are embedded.<br />
Seed (cross-secti<strong>on</strong>): the epidermis of the testa is composed of radially<br />
el<strong>on</strong>gated rectangular cells filled with mucilage. The walls are thickened in<br />
a U-shape, the thickest wall being the exterior <strong>on</strong>e; the mucilage is radially<br />
striated, and in the centre of the cell is a small lumen. Below the epidermis<br />
are several layers of polyg<strong>on</strong>al cells with thick, brown, occasi<strong>on</strong>ally reticulately<br />
thickened walls. These cells are 250–350 m l<strong>on</strong>g and approximately<br />
80 m broad. The innermost layer of the testa c<strong>on</strong>sists of compressed, rectangular<br />
cells with sinuous l<strong>on</strong>gitudinal walls. The voluminous endosperm<br />
is made up of small polyg<strong>on</strong>al and oil-c<strong>on</strong>taining cells (1).<br />
Powdered plant material<br />
Numerous fragments of the exocarp with colourless cell walls and violet<br />
c<strong>on</strong>tents; thin-walled parenchyma of the mesocarp; thickened and pitted<br />
cells of the testa; oil droplets (1).<br />
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