GreenSep SFC Column Information - Canadian Life Science

SFC (ES Industries)
a-Chiral GreenSep SFC Columns
Supercritical fluid chromatography (SFC) is a powerful chromatographic
technique for the separation of complex mixtures. It
has been useful in the areas of preparative chromatography and
rapid analysis chromatography. SFC is a “green” chromatographic
technique where the main component of the mobile
phase is CO 2 . A CO 2 based mobile phase composition is a
“green” alternative to conventional LC mobile phases. In
addition CO 2 provides for the rapid recovery of purified components.
The use of CO 2 based mobile phases enables the use of
high performance preparative columns (10mm – 50 mm ID) with
a variety of particle sizes from 3 – 20µm. Many SFC separations
have been forced to utilize older types of stationary phases from
“normal-phase” LC such as unmodified silica, diol, amino and
cyano.
These phases are poorly adapted to SFC and present a number
of limitations for SFC separations. Limitations include: low
capacity, poor selectivity, and poor peak shape for SFC.
ES Industries has developed a new line of GreenSep
stationary phases specifically engineered for SFC separations.
GreenSep columns (10mm to 50mm ID) have been designed
to deliver high performance SFC separations. Columns from
analytical to preparative sizes are available for all SFC phases
including our new high performance 15mm ID columns and
particle size from 3 – 20µm.separations.
GreenSep phases are also available for large scale
purification.
The following specialty SFC stationary phases were designed to
provide superior separation selectivity, peak shape and loading
capacity without the addition of peak shape modifiers. Mobile
phase composition and fraction collection is greatly simplified
without the use of additives. These GreenSep phases can
easily replace conventional stationary phases used in SFC and
deliver superior performance.
Figure 1. Basic compounds
GreenSep Ethyl Pyridine does not require the addition of
amine to the mobile phase (see figure 1 of the superior peak
shape performance obtainable) thus making it the SFC column
of choice for the retention and separation of chemical containing
strong amine groups.
GreenSep Pyridyl Amide does not require the addition of TFA
or an amine to the mobile phase to achieve superior peak performance
(see figure 2).
GreenSep DEAP is the column of choice for the retention and
rapid separation of chemical containing strong amine groups
without the addition of amine modifiers to the mobile phase.
The following specialty SFC stationary phases have proven
superior to conventional stationary phases (such as diol, cyano,
etc.) in the areas of separation selectivity and loading capacity.
Figure 2. Basic and acidic compounds
GreenSep Nitro and GreenSep PFP have been specifically
designed for the separation of geometrical isomers as well as
diastereomers. They are the column of choice in separating compounds
that contain aromatic groups, polarizable electrons and
conjugate systems. They are available in analaytical columns,
preparative columns and bulk media.
In addition, GreenSep PFP is useful for the separation of halogenated
compounds. In many cases GreenSep PFP provides
orthogonal separations when compared to GreenSep Nitro.
GreenSep Amino Phenyl has been designed for the separation
of amines, alcohols and acids by SFC without the use of
additives. As with other specialty SFC phases developed at ES
industries GreenSep Amino Phenyl provides superior performance
when compared against conventional SFC phases.
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SFC (ES Industries)
Ordering Information - GreenSep
Column Dimensions (mm)
GreenSep Phase
5µm 1 30 x 4.6 2 50 x 4.6 2 100 x 4.6 2 150 x 4.6 2 250 x 4.6 2 300 x 4.6 2
$525 $525 $556 $604 $653 $714
Amino Phenyl ES185291-GS-APH ES115291-GS-APH ES125291-GS-APH ES135291-GS-APH ES155291-GS-APH ES165291-GS-APH
$525 $525 $556 $604 $653 $714
DEAP - - ES125291-GS-DEAP ES135291-GS-DEAP ES155291-GS-DEAP -
Ethyl Pyridine ES185291-GS-PYE ES115291-GS-PYE ES125291-GS-PYE ES135291-GS-PYE ES155291-GS-PYE ES165291-GS-PYE
PFP ES185221-GS-PFP ES115221-GS-PFP ES125291-GS-PFP ES135291-GS-PFP ES155291-GS-PFP ES165291-GS-PFP
Pyridyl Amide ES185291-GS-PYA - ES125291-GS-PYA ES135291-GS-PYA ES155291-GS-PYA ES165291-GS-PYA
- $525 $556 $604 $653 -
Nitro - ES115291-GS-NO2 ES125291-GS-NO2 ES135291-GS-NO2 ES155291-GS-NO2 -
1 Some GreenSep phases also available in 1.8µm, 3µm and 10µm. Please enquire.
2 Use with holder ES300100 ($110) and Guard Cartridge ES300101-XXX , 5µm - pack of 5 (XXX - Please specify required phase). ($281)
GreenSep phases are also available for large scale purification.
Please contact Canadian Life Science for a catalogue
or technical papers on the complete ES Industries product range.
SFC - The Green Chemistry!
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