full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 5 (3) 1282-1297 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
1287<br />
Table 1 The antimicrobial activities <strong>of</strong> actinomycetes isolate No.9<br />
Tested microbial pathogen<br />
Diameter <strong>of</strong> inhibition zone (mm)<br />
Escherichia coli ATCC 25922 20<br />
Bacillus subtilis NRRL B-4219 30<br />
Pseudomonas aeruginosae ATCC 27953 -<br />
Staphylococcus aureus ATCC 29213 20<br />
C<strong>and</strong>ida albicans ATCC 10231 30<br />
Saccharomyces cerevisiae ATCC 10275 25<br />
Aspergillus niger NRRL 599 30<br />
Aspergillus flavus NRRL 1957 -<br />
Alcaligenes faecalis NRRL B-170 30<br />
Micrococcus luteus NRRL B-287 10<br />
Trycophyton mentagrophytes ATCC 9533 14<br />
Table 2 Identification criteria <strong>of</strong> actinomycetes isolate no. 9 (Nocardioides luteus)<br />
Experimental Parameter Character Experimental Parameter Character<br />
Aerial mycelia color Grey L-diaminopimelic acid in whole cell hydrolysate +<br />
Vegetative mycelia color Yellow-Brown Growth on sole carbon sources (1.0 % w/v)<br />
Melanin on tyrosine agar + Sucrose +<br />
Degradation <strong>of</strong> - Lactose +<br />
Casein + Salicin +<br />
Xanthene - D-Fructose +<br />
Hypoxanthene- Galactose +<br />
Urea + D-Mannose +<br />
Growth at D-Mannitol +<br />
45 o C - Rafinose +<br />
10 o C + D-Xylose +<br />
pH 4.3 + L-Arabinose +<br />
Growth in the presence <strong>of</strong> (%w/v)<br />
Cellobiosem<br />
NaCl (4) + Inositol +<br />
NaCl (7) + Rhamnose +<br />
NaCl (10) + Glucose +<br />
Crystal violet (0.0001) - Sodium acetate (0.1 %) +<br />
Phenol (0.1) Sodium citrate (0.1%) +<br />
Resistance to antibiotics Sodium propionate (0.1 % ) +<br />
Gentamicin (10 µg) Growth on sole nitrogen source (0.1 %)<br />
Streptomycin (10 µg) - L-Arginine +<br />
Neomycin (10 µg) - L-Histidine +<br />
Penicillin G (10 units) - L-Cysteine +<br />
Rifampicin (5 µg) - L-Valine +<br />
Tetracycline (30 µg) - L-Phenyl alanine +<br />
Tobramycin (10 µg) + Potassium nitrate +<br />
Erythromycin (15 µg) - L-Threonine +<br />
Bacitracin (10 µg) - L-Serine +<br />
Chloramphenicol (30 µg) - L-Methionine +<br />
Novobiocin (5 µg) -<br />
El-Refai et al