full issue - Association of Biotechnology and Pharmacy

More documents

Recommendations

Info

Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1273 -1281 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1281 Appl Catalysis B: Environmental, 28:83– 99. 21. Fabbrini, M., Galli, C. and Gentili, P. (2002). Comparing the catalytic efficiency of some mediators of laccase. J Mol Cat B: Enzymatic, 16: 231–240. 22. Matto, M. and Hussain, Q. (2007). Decolorization of direct dyes by salt fractionated turnip proteins enhanced in the presence of hydrogen peroxide and redox mediators. Chemosphere, 69: 388–345. 23. d’Acunzo, F. and Galli, C. (2003). First evidence of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin models. European J Biochemistry, 270: 3634–3640 24. Murugesan, K., Nam, I. H., Kim, Y. M. and Chang, Y. S. (2007).Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture. Enzyme and Microbial Technology, 40:1662–1672. 25. Kulshrestha, Y. and Husain, Q. (2007). Decolorization and degradation of acid dyes mediated by salt fractionated turnip Brassica rapa peroxidases. Toxicological and Environmental Chemistry, 89: 255– 267. 26. Vazquez-Duarte, H., Garcia-Almendarez, B., Regalado, C. and Whitaker, J. R. (2001). Purification and properties of a neutral peroxidase from turnip Brassica napus L varpurple top white globe roots. Journal of Agricultural and Food Chemistry, 49:4450– 4456. 27. Camarero, S., Ibarra, D., Martinez, M.J. and Martinez, A.J. (2005). Lignin derived compounds as efficient laccase mediators or decolorization of different types of recalcitrant dyes. Applied Environmental Microbiology, 71: 1775–1784. 28. Kahru, A., Pollumaa, L., Reiman, R., Ratsep, A., Liiders, M. and Maloveryan, A. (2000). The toxicity and biodegradability of eight main phenolic compounds characteristic to the oil-shale industry wastewater: a test battery approach. Environmental Toxicology, 15:431–442. 29. Soares, G.M., De Amorim, M.T. and Costa- Ferreira, M. (2001). Use of laccase together with redox mediators to decolorize Remazol Brilliant Blue R. J Biotechnology, 89: 123–129. 30. Claus, H., Faber, G. and Konig, H. (2002). Redox-mediated decolorization of synthetic dyes by fungal laccases. Applied Microbiology and Biotechnology, 59: 672– 678. Simple approach to reactive dye decolorization
Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1273 1282-1297 -1281 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) Studies on the Production of Actinomycin by Nocardioides luteus, a Novel Source 1282 El-Refai, H.A. 1* , AbdElRahman 1 , H.Y., Abdulla, H. 2 , Hanna, Atef G. 1 , Hashem, A.H. 3 , El-Refai A.H. 1 and Ahmed, E.M. 1 1 Chemistry of Natural and Microbial Products Dept., National Research Center, Dokki, Cairo, Egypt 2 Dept. of Botany, Faculty of Science, Suez Canal University, Egypt 3 Dept. of Microbiology, Faculty of Pharmacy, Cairo University, Egypt *For Correspondence - dr.heba_ar@yahoo.com Abstract Efforts have been devoted to explore the antimicrobial activity of local egyptian actinomycetes isolates. 127 pure actinomycetes isolates were recovered from the stones at the location of the tomb of Tell Basta (the site of the ruins of the old temple of Bast) which is southeast of the Nile Delta at the township of Zagazig in Egypt. The most competent isolate showed promising activity against some pathogenic yeasts, fungi and bacteria. It was identified as Nocardioides luteus. Using 2 ml of 2 days old inoculum of Nocardioides luteus in fermentation medium consisting of (g/l): starch, 10; yeast extract, 4; NaCl, 10; K 2 HPO 4 , 2; MgSO 4 .7H 2 O, 0.05; CaCO 3 , 0.02 and FeSO 4 .7H 2 O, 0.01, adjusted before autoclaving at pH 7 and incubated for 7 days with shaking rate 200 rpm resulted in maximum antimicrobial activity against Candida albicans ATCC 10231. The minimum inhibitory concentration (MIC) of the culture was determined to be 0.4 µg/ml. Trials have been conducted to separate and identify the active compound(s) responsible for the anticandidal activity. Based on the spectroscopic data from UV, IR, 1 H-NMR and 13 C-NMR spectra, the isolated compounds were identified as derivatives of actinomycins class. To our knowledge, this is the first time not only to study systematically the bioactivities of Nocardioides luteus; but also to report actinomycin production by a Nocardioides spp. Cytotoxicity of both the culture filtrate extract and the impure compound were tested against different tumor cell lines where they showed remarkable cytotoxicity against all challenged cell lines. Keywords: actinomycetes, antimicrobial, actinomycin, cytotoxicity. Introduction Actinomycetes are a group of bacteria, whose members are famous for producing antibiotics for medicinal use. They provid us with a large database of compounds with different structures; including aminoglycosides, anthracyclines, glycopeptides, â-lactams, macrolides, nucleosides, peptides, polyenes, polyketides, quinones, actinomycins, tetracyclines and even alkaloids. Only a very minute quantity of terrestrial and marine actinomycetes were actually isolated and tested for antibacterial activity. In the time interval between 1981-2006, 19 out of 24 naturally originating compounds acting as the starting material of marketed drugs, were isolated from soil actinomycetes (1,2,3). Within the actinomycetes, Streptomyces is the most abundant genus that accounts for 70- 80% of secondary metabolites, with smaller contributions from genera such as Saccharopolyspora, Amycolatopsis, Studies on the production of actinomycin by Nocardioides luteus
Page 3 and 4:
ISSN 0973-8916 Current Trends in Bi
Page 5 and 6:
Current Trends in Biotechnology and
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32: Current Trends in Biotechnology and
Page 61 and 62: β Current Trends in Biotechnology
Page 81: Current Trends in Biotechnology and
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175:
(December 7-9, 2011) Venue: Karunya
show all

full issue - Association of Biotechnology and Pharmacy

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?