full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 5 (3) 1362-1371 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
1367<br />
Fig. 2. Comparison <strong>of</strong> Total reducing power, Nitric Oxide<br />
<strong>and</strong> Super-oxide scavenging activity <strong>of</strong> 100mcg/ml <strong>of</strong> AA,<br />
BHA, Alcohol <strong>and</strong> Chlor<strong>of</strong>orm Extract <strong>of</strong> the root <strong>of</strong> X.<br />
6 hr<br />
24 hr<br />
48 hr<br />
Fig. 4. Effects <strong>of</strong> Chlor<strong>of</strong>orm <strong>and</strong> ethanol extracts <strong>of</strong> root<br />
<strong>of</strong> X.strumarium on total cholesterol <strong>and</strong> low density lipids<br />
levels in triton-induced hyperlipidemic rats<br />
Fig. 3. Comparison <strong>of</strong> Total reducing power, Nitric Oxide<br />
<strong>and</strong> Super-oxide scavenging activity <strong>of</strong> 500mcg/ml <strong>of</strong> AA,<br />
BHA, Alcohol <strong>and</strong> Chlor<strong>of</strong>orm Extract <strong>of</strong> the root <strong>of</strong><br />
X. strumarium<br />
inhibited by BHA (st<strong>and</strong>ard) <strong>and</strong> extracts from<br />
100µg/ml. Both the extracts produced significant<br />
superoxide radical scavenging activity in a<br />
concentration-dependent manner. Chlor<strong>of</strong>orm<br />
extract showed the lowest IC value (418 ± 4.15)<br />
50<br />
which is better than BHA (415.14 ± 5.98).<br />
Antihyperlipidemic activity: The non ionic<br />
detergent, Triton WR-1339, has been widely used<br />
to block the uptake <strong>of</strong> triacyl glycerol rich<br />
lipoprotein from plasma by peripheral t<strong>issue</strong>s in<br />
order to produce acute hyperlipidemia in animal<br />
models which are <strong>of</strong>ten used for a number <strong>of</strong><br />
objectives. The parenteral administration <strong>of</strong><br />
Triton induces hyperlipidemia in adult rats,<br />
where the blood cholesterol <strong>and</strong> triglycerides<br />
levels were reached maximum in 20hrs, followed<br />
by a decline to normal values (12-14).<br />
Administration <strong>of</strong> Triton resulted in increase in<br />
serum levels <strong>of</strong> cholesterol, triglycerides, VLDL,<br />
<strong>and</strong> LDL. A significant reversal in serum levels<br />
<strong>of</strong> cholesterol, triglycerides, VLDL, <strong>and</strong> LDL<br />
levels was noticed in the animals treated with X.<br />
strumarium root extracts when compared with<br />
the control group (Table-3). Simvastatin<br />
(st<strong>and</strong>ard) produced maximum cholesterol- <strong>and</strong><br />
LDL-lowering effect at both 6 h <strong>and</strong> 24 h. ethanol<br />
extract 200 <strong>and</strong> Chlor<strong>of</strong>orm extract 400 produced<br />
a significant decrease in serum cholesterol <strong>and</strong><br />
Sridharamurthy et al