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Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1362-1371 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1365 Chemicals: The chemicals DPPH (1,2-diphenyl- 2-picrylhydrazyl), N-(1-Naphthyl) thylenediamine dihydrochloride, Triton WR- 1339, NADH, SNP, phenazine methosulphate, trichloro acetic acid, and potassium ferricyanide were purchased from Sigma Chemicals, St Louis, MO, USA. All other chemicals and reagents used were of analytical grade. UV-1700 Shimadzu UV-Vis spectrophotometer was used for in vitro anti-oxidant studies. Statistical analysis: All the values are presented as mean SD. Data were statistically analyzed by one-way ANOVA followed by post hoc test; P values < 0.05 were considered as statistically significant. Linear regression analysis was used for calculation of IC 50 . of the standards BHT and ascorbic acid and were found to have IC (mean ± SD) of 50 8.89±1.10,9.15±1.21,10.18±1.50,9.23±0.91, respectively (Fig. 1; Table-1). DPPH radical scavenging activity: The chloroform and ethanol extract produced significant DPPH radical scavenging activity. The IC (mean ± SD) of ascorbic acid, BHT 50 and other two extracts were found to be 27.35±1.20, 26.01±0.93, 29.81±0.95, 24.85±2.49 respectively (Table-1; Fig.1) Acute toxicity studies: There was no mortality and noticeable behavioral changes in all the groups tested. The chloroform and ethanol extracts of root of X.strumarium were found to be safe up to 2000 mg/kg body weight. Results and Discussions Hydrogen peroxide scavenging activity: At 125 µg/ml concentration, the chloroform extract and ethanol extract 100 µg/ml produced H 2 O 2 scavenging activity comparable (P < 0.05) to that Fig. 1. Free radical activity of different extracts of X. strumarium Table 1. Free radical scavenging activity of different activities of X. Strumanium Sridharamurthy et al
Current Trends in Biotechnology and Pharmacy Vol. 5 (3) 1362-1371 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online) 1366 Nitric oxide radical scavenging activity: Scavenging of nitric oxide by various extracts was found to be concentration dependent. Maximum inhibition of nitric oxide formation was produced by alcoholic extract and had IC 50 of 495.30±3.09 as against 415.14± 5.98 for BHA (Fig. 1; Table-2). Total reducing power: From 100 µg/ml, chloroform and ethanol extracts and standardascorbic acid and BHA showed reductive capabilities. At 500 µg/ ml, the reducing power of standards and extracts showed the following order: BHA > ethanol extract > Ascorbic acid > chloroform. Reducing power of ethanol and chloroform extracts at 500µg/ml was comparable (P < 0.05) to that of ascorbic acid (Table-1). Superoxide anion radical scavenging activity: Superoxide anion radical generation was Table 2. Total reducing power, nitric oxide and super oxide scavenging activity of different concentrations of AA.BHA.XSCA,XSAE In-vitro antioxidant and antilipidemic activities of Xanthium strumarium L.
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Page 175: (December 7-9, 2011) Venue: Karunya
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