full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 5 (3) 1362-1371 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
1365<br />
Chemicals: The chemicals DPPH (1,2-diphenyl-<br />
2-picrylhydrazyl), N-(1-Naphthyl)<br />
thylenediamine dihydrochloride, Triton WR-<br />
1339, NADH, SNP, phenazine methosulphate,<br />
trichloro acetic acid, <strong>and</strong> potassium ferricyanide<br />
were purchased from Sigma Chemicals, St Louis,<br />
MO, USA. All other chemicals <strong>and</strong> reagents used<br />
were <strong>of</strong> analytical grade. UV-1700 Shimadzu<br />
UV-Vis spectrophotometer was used for in vitro<br />
anti-oxidant studies.<br />
Statistical analysis: All the values are presented<br />
as mean SD. Data were statistically analyzed<br />
by one-way ANOVA followed by post hoc test;<br />
P values < 0.05 were considered as statistically<br />
significant. Linear regression analysis was used<br />
for calculation <strong>of</strong> IC<br />
50 .<br />
<strong>of</strong> the st<strong>and</strong>ards BHT <strong>and</strong> ascorbic acid <strong>and</strong> were<br />
found to have IC (mean ± SD) <strong>of</strong><br />
50<br />
8.89±1.10,9.15±1.21,10.18±1.50,9.23±0.91,<br />
respectively (Fig. 1; Table-1).<br />
DPPH radical scavenging activity: The<br />
chlor<strong>of</strong>orm <strong>and</strong> ethanol extract produced<br />
significant DPPH radical scavenging activity.<br />
The IC (mean ± SD) <strong>of</strong> ascorbic acid, BHT<br />
50<br />
<strong>and</strong> other two extracts were found to be<br />
27.35±1.20, 26.01±0.93, 29.81±0.95, 24.85±2.49<br />
respectively (Table-1; Fig.1)<br />
Acute toxicity studies: There was no mortality<br />
<strong>and</strong> noticeable behavioral changes in all the<br />
groups tested. The chlor<strong>of</strong>orm <strong>and</strong> ethanol<br />
extracts <strong>of</strong> root <strong>of</strong> X.strumarium were found to<br />
be safe up to 2000 mg/kg body weight.<br />
Results <strong>and</strong> Discussions<br />
Hydrogen peroxide scavenging activity: At 125<br />
µg/ml concentration, the chlor<strong>of</strong>orm extract <strong>and</strong><br />
ethanol extract 100 µg/ml produced H 2<br />
O 2<br />
scavenging activity comparable (P < 0.05) to that<br />
Fig. 1. Free radical activity <strong>of</strong> different extracts <strong>of</strong><br />
X. strumarium<br />
Table 1. Free radical scavenging activity <strong>of</strong> different activities <strong>of</strong> X. Strumanium<br />
Sridharamurthy et al