full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 5 (3) 1362-1371 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)
1365
Chemicals: The chemicals DPPH (1,2-diphenyl-
2-picrylhydrazyl), N-(1-Naphthyl)
thylenediamine dihydrochloride, Triton WR-
1339, NADH, SNP, phenazine methosulphate,
trichloro acetic acid, and potassium ferricyanide
were purchased from Sigma Chemicals, St Louis,
MO, USA. All other chemicals and reagents used
were of analytical grade. UV-1700 Shimadzu
UV-Vis spectrophotometer was used for in vitro
anti-oxidant studies.
Statistical analysis: All the values are presented
as mean SD. Data were statistically analyzed
by one-way ANOVA followed by post hoc test;
P values < 0.05 were considered as statistically
significant. Linear regression analysis was used
for calculation of IC
50 .
of the standards BHT and ascorbic acid and were
found to have IC (mean ± SD) of
50
8.89±1.10,9.15±1.21,10.18±1.50,9.23±0.91,
respectively (Fig. 1; Table-1).
DPPH radical scavenging activity: The
chloroform and ethanol extract produced
significant DPPH radical scavenging activity.
The IC (mean ± SD) of ascorbic acid, BHT
50
and other two extracts were found to be
27.35±1.20, 26.01±0.93, 29.81±0.95, 24.85±2.49
respectively (Table-1; Fig.1)
Acute toxicity studies: There was no mortality
and noticeable behavioral changes in all the
groups tested. The chloroform and ethanol
extracts of root of X.strumarium were found to
be safe up to 2000 mg/kg body weight.
Results and Discussions
Hydrogen peroxide scavenging activity: At 125
µg/ml concentration, the chloroform extract and
ethanol extract 100 µg/ml produced H 2
O 2
scavenging activity comparable (P < 0.05) to that
Fig. 1. Free radical activity of different extracts of
X. strumarium
Table 1. Free radical scavenging activity of different activities of X. Strumanium
Sridharamurthy et al