full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
full issue - Association of Biotechnology and Pharmacy
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 5 (3) 1206-1232 July 2011, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
1215<br />
UvrA2B complex formation<br />
Block domains 2 & 4 <strong>of</strong> UvrB<br />
The NER pathway is interrupted<br />
UvrC protien dissociaties<br />
The new piece <strong>of</strong> is<br />
str<strong>and</strong> is synthesized<br />
by the action<br />
<strong>of</strong> helicase II <strong>and</strong><br />
DNA Polymerase I<br />
The new str<strong>and</strong> is<br />
attached to the rest<br />
<strong>of</strong> the chain by the<br />
action <strong>of</strong> DNA ligase<br />
Fig. 3. (Left) Schematic representation <strong>of</strong> the nucleotide<br />
excision repair system. After the recognition process, the<br />
UvrA2B complex is formed. Then the UvrA dimer<br />
dissociates the complex allowing the formation <strong>of</strong> the preincision<br />
complex <strong>and</strong> the cutting <strong>of</strong> the damaged DNA by<br />
the action <strong>of</strong> UvrC <strong>and</strong> UvrB. While UvrC dissociates<br />
allowing the binding <strong>of</strong> UvrD <strong>and</strong> DNA polymerase. The<br />
new piece <strong>of</strong> str<strong>and</strong> is synthesized <strong>and</strong> finally attached to<br />
the rest <strong>of</strong> the chain by the action <strong>of</strong> DNA ligase.<br />
(Right) Schematic representation <strong>of</strong> the proposed target<br />
to be studied for further underst<strong>and</strong>ing <strong>of</strong> the NER<br />
mechanism. Through the blocking or inhibition <strong>of</strong> the<br />
domains 2&4 <strong>of</strong> the UvrB , the recognition process is<br />
interrupted <strong>and</strong> the ability <strong>of</strong> DNA repair using NER<br />
becomes ineffective.<br />
Coronado et al